2465 Results for: "STEMCELL Technologies"

Supplier: STEMCELL Technologies

Interleukin 9 (IL-9) has pleiotropic functions in the immune system and signals through its specific IL-9 receptor (IL-9R) (Renauld et al.). IL-9/IL-9R signaling pathway mainly targets the downstream activation of JAK/STAT (janus kinase/signal transducer and activator of transcription) and subsequent phosphorylation cascades initiated by multiple kinases including IRS–PI3K–PKB (insulin receptor substrate, phosphatidyl-inositol 3-kinases, protein kinase-B) and ERK (Knoops and Renauld; Fontaine et al.). IL-9 has been shown to have a role in Th1/Th17-mediated inflammation and in regulatory T cell responses (Singh et al.; Goswami and Kaplan). IL-9/IL-9R signaling pathway represents a novel endogenous anti-apoptotic mechanism for cortical neurons (Fontaine et al.). IL-9 secreting T cells, termed Th9 cells, contribute to both effective immunity and immunopathological disease, and have been shown to have a role in the treatment of allergic and autoimmune disease (Kaplan et al.).

Supplier: STEMCELL Technologies

Interleukin 13 (IL-13) is a cytokine important in type 2 immune responses and is expressed by T helper type 2 (Th2) cells and group 2 innate lymphoid cells (ILC2s) (Pulendran and Artis). IL-13 binds a receptor composed of IL-4Ra and IL-13Ra1 or IL-13Ra2 (Wynn 2003). IL-13 receptor is expressed on B cells and promotes B cell proliferation, induces class switching to IgG4 and IgE, and functions in the recruitment and activation of IgE-producing B cells (Hershey). The receptor is also expressed on basophils, eosinophils, mast cells, endothelial cells, fibroblasts, monocytes, macrophages, respiratory epithelial cells, and smooth muscle cells (Hershey). Signaling through the IL-13 receptor activates the JAK/STAT and IRS-1/IRS-2 pathways. in vivo, IL-13 has a role in resistance to extracellular helminth parasites by regulating gastrointestinal parasite expulsion, as well as in airway hyperresponsiveness, allergic inflammation, tissue remodeling, tumor cell growth, and fibrosis (Wynn 2015). Secreted IL-13 is a protein consisting of 112 amino acids with a molecular mass of 10 kDa (Hershey). Human IL-13 is not species-specific but has greater activity on human cells compared to mouse cells (Hershey).

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GRO (growth-regulated oncogene)-beta or CXCL2 is a member of the CXC family, which plays an integral role in recruitment and activation of neutrophils and basophils in response to tissue injury and microbial infection. GRO-beta and GRO-gamma are closely related to GRO-alpha and share 90% and 86% amino acid sequence homology, respectively, with GRO-alpha. Receptor-binding studies have demonstrated that GRO-alpha, -beta, and -gamma signal mainly through G protein-coupled receptors CXCR1 and CXCR2 (Ahuja and Murphy). GRO-beta is expressed in epithelial cells, monocytes, fibroblasts, and melanocytes and is further induced during inflammatory, epithelialization, and angiogenic processes, for example during the wound healing process of human burn wounds (Zaja-Milatovic and Richmond). GRO-beta also stimulates mitogenesis in certain human melanoma cells (Unemori et al.).

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Stromal cell-derived factor 1 alpha (SDF-1α) is a member of the CXC group of chemokines that binds to the G-protein coupled receptor, CXCR4, to regulate migration, proliferation, differentiation, and survival of many cell types including hematopoietic stem cells, B cells, and T cells. It is produced by bone marrow stromal cells, osteoblasts, endothelial cells, and neuronal cells. SDF-1α was first identified as the pre-B-cell growth-stimulating factor (PBSF) in the mouse bone marrow-derived stromal cell line, PA6, in the growth of B cell precursors (Hayashi et al.). SDF-1α primarily regulates cell motility during development and adulthood, including the homing of hematopoietic stem cells and neutrophils to fetal bone marrow during ontogeny (Ara et al. 2003a) and the recruitment of endothelial progenitor cells from bone marrow during angiogenesis in adulthood (Zheng et al.). In addition to its role in hematopoiesis, the SDF-1α/CXCR4 signaling pathway is also essential for the homing of primordial germ cells to gonads (Ara et al. 2003b), the migration of granule cells in the cerebellum during neurogenesis (Zou et al.), and the migration of breast cancer cells to sites of metastasis (Muller et al.).

Supplier: STEMCELL Technologies

Stimulating T cells with Candida (MP65) peptide pool releases downstream cytokines and upregulates activation markers, enabling antigen-specific T cells to be detected or isolated for analysis. Candida peptide pool is a lyophilised mixture of 92 peptides from the mannoprotein MP65 of Candida albicans (yeast), and consists of 15-mer peptides with 11-amino-acid overlaps that cover amino acids 1 to 379 on MP65. MP65 contributes to cell wall integrity and epithelia adherence (Sandini et al.), and is a potent T cell immunogen (Gomez et al.). Viral peptide pools are useful for a broad range of applications, including vaccine development, immunological research, and diagnostic assay development.

Supplier: STEMCELL Technologies

Methylcellulose-based medium with recombinant cytokines for human cells. 1×100 ml bottle.

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Immunomagnetic cell isolation kit using particle release technology.

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Immunomagnetic isolation of untouched non-human primate T cells

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Immunomagnetic positive selection of human cells labeled with PE-conjugated antibodies.

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Immunomagnetic positive selection of human cells labeled with FITC-conjugated antibodies.

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Measure and transfer liquids precisely and safely using Corning® Lambda™ Plus multi-channel pipettors.

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Column-free immunomagnetic positive selection of human CD66b+ cells for HLA analysis.

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Immunomagnetic negative selection kit.

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Column-free immunomagnetic positive selection of human B cells for HLA analysis.

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Immunomagnetic positive selection of human CD33+ and CD66+ cells (e.g. myeloid cells).

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Cell culture medium for establishment and maintenance of mouse pancreatic exocrine organoids.

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Serum-free medium kit for highly efficient SMAD inhibition-mediated neural induction of human ES and iPS cells.

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Defined cell culture medium for expansion of reset naïve human embryonic stem (ES) and induced pluripotent stem (iPS) cells.

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Defined medium kit for transgene-free induction of reset naïve human pluripotent stem cells - 1 Kit

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SARS-CoV-2 Recombinant Spike Protein, aa16-685 is expressed in HEK293 cells and is one of four structural proteins encoded by the SARS-CoV-2 genome. The Spike Protein plays a key role in attachment to host cells, allowing invasion through clathrin-mediated endocytosis. The Spike Protein can be cleaved by host cell proteases after aa685 to yield the N-terminal S1 subunit and C-terminal S2 region. The S1 subunit is responsible for interacting with the host cell receptor (angiotensin-converting enzyme II) through a receptor-binding domain that is highly conserved with SARS-CoV. The S1 subunit has two conformations: a ‘down’ conformation in which the receptor is inaccessible, and an ‘up’ conformation in which the receptor is accessible. These conformational changes are key for monoclonal antibody drugs and vaccine development. SARS-CoV-2 Recombinant Spike Protein contains a polyhistidine tag at the amino terminus; it also contains a FLAG tag at the carboxy terminus.

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Mouse monoclonal antibody against human, rhesus, cynomolgus CD9, unconjugated.

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A cellular protein for detection of apoptotic cells, APC-conjugated.

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Influenza (HLA class I control) peptide pool is a lyophilised mixture of 17 peptides from the influenza virus. The pool consists of defined HLA class I-restricted T cell epitopes, and can be used as an antigen-specific positive control to stimulate T cells.

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The EBV (EBNA-1) Peptide pool is a lyophilised mixture of 158 peptides from Epstein-Barr nuclear antigen 1 (EBNA-1) of Epstein-Barr virus (EBV; strain B95-8). EBNA-1 plays a critical role in the stable latent infection by EBV and may contribute to the survival of EBV-infected cells through its interaction with ubiquitin carboxyl-terminal hydrolase 7 (USP7). The pool consists of 15-mer peptides with 11-amino-acid overlaps that cover amino acids 1 to 641 on EBNA-1.

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The CMV (pp65) peptide pool is a lyophilised mixture of 138 peptides from the 65 kDa phosphoprotein (pp65) of human cytomegalovirus (CMV; strain AD169). pp65 has been shown to counteract the induction of interferon response by preventing the activation of interferon response factor 3 (IRF-3). pp65 is also important in inducing transcription of viral immediate-early promoter. The pool consists of 15-mer peptides with 11-amino-acid overlaps that cover amino acids 1 to 561 on pp65.

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Serum-free and xeno-free base medium for the expansion of B cells.

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Serum-free and conditioned medium-free cell culture medium for establishment and maintenance of human intestinal organoids.

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Cell culture medium kit for robust generation of ventral forebrain neural organoids from human pluripotent stem cells.

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The SARS-CoV-2 (VME1) peptide pool is a lyophilised mixture of 53 peptides from the SARS-CoV-2 membrane protein VME1. VME1 is a structural protein that is common to all coronaviruses, and it plays a critical role in viral assembly. The pool consists of 15-mer peptides with 11-amino-acid overlaps that cover amino acids 1 to 222 on VME1.

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The SARS-CoV-2 (spike protein) peptide pool is provided as two lyophilised mixtures (subpools) from the SARS-CoV-2 spike protein. Each subpool contains 158 peptides, for a total of 316 peptides. The virus attaches to the cell membrane of the host through the interaction between spike protein and angiotensin-converting enzyme 2 (ACE2) receptor, and the spike protein plays a critical role in viral entry. The subpools consist of 15-mer peptides with 11-amino-acid overlaps that cover amino acids 1 to 1273 on the spike protein.