1072 Results for: "SIGMA ALDRICH MICROSCOPY"

Supplier: SIGMA ALDRICH MICROSCOPY

Esterases are ubiquitous in nature and encompass a series of different enzymes acting upon select substrates.

Supplier: SIGMA ALDRICH MICROSCOPY

Auramine O, Sigma-Aldrich®

Supplier: SIGMA ALDRICH MICROSCOPY

Brilliant green ≥90% (dye content), certified by the Biological Stain Commission, Sigma-Aldrich®

Supplier: SIGMA ALDRICH MICROSCOPY

Propyl-3,4,5-trihydroxybenzoate ≥98% (by HPLC) for histology and pathology, for diagnostic, Sigma-Aldrich®

Supplier: SIGMA ALDRICH MICROSCOPY

Carbazole is an aromatic heterocyclic organic molecule.

Supplier: SIGMA ALDRICH MICROSCOPY

Formaldehyde is both a non-coagulant and an additive fixative. Formaldehyde is known to penetrate tissue quickly, but the fixation process may take a long time to form the various cross links with tissue proteins. Formalin fixation is thought to form cross links between the aldehydes and the proteins, creating a gel, thus retaining cellular constituents in their in vivo relationship. Once properly fixed, the tissue should be able to withstand the subsequent stages of tissue processing or staining. Although there is no true universal fixative, 10% neutral buffered formalin (NBF) is perhaps the most commonly used fixative throughout the world for light microscopy and is a somewhat forgiving fixative. Of the dozens and dozens of available fixatives, it is hard to imagine a fixative more commonly used than 10% NBF. 10% neutral buffered formalin is well suited for large throughput laboratories, and requires a relatively short period of fixation, but can also be used for the long-term storage of tissue.

Supplier: SIGMA ALDRICH MICROSCOPY

Kit component in the Masson Trichrome procedure, catalog number HT15

Supplier: SIGMA ALDRICH MICROSCOPY

Yellow counterstain suitable for a wide range of histological staining procedures.

Supplier: SIGMA ALDRICH MICROSCOPY

Silver stain is an aid in distinguishing fungi, basement membrane and opportunistic organisms such as Pneumocystis carinii in tissue sections or smears.

Supplier: SIGMA ALDRICH MICROSCOPY

Intended for use in Sigma Gram Stain Procedure, HT90.

Supplier: SIGMA ALDRICH MICROSCOPY

Canada balsam, Sigma-Aldrich®

Supplier: SIGMA ALDRICH MICROSCOPY

3,3′-Diaminobenzidine tetrahydrochloride hydrate has been used in immunohistological staining procedure and in situ hybridisation. DAB (3,3′-Diaminobenzidine) is utilised in many applications for the visualisation of peroxidase activity. In the peroxidase reaction, DAB serves as a hydrogen donor in the presence of peroxide. The oxidised DAB forms an insoluble brown end-product for use in immunohistological and immunoblotting staining procedures.

Supplier: SIGMA ALDRICH MICROSCOPY

Methylene blue, Neisser formulation. Well developed granules of volutin (polyphosphate) may be seen in unstained wet preparations as round refractive bodies within the bacterial cytoplasm. With methylene blue they stain metachromatically, a reddish purple color.

Supplier: SIGMA ALDRICH MICROSCOPY

Naphthol AS phosphate disodium salt is intended for use as a substrate for the histochemical demonstration of acid and alkaline phosphatase activities.

Supplier: SIGMA ALDRICH MICROSCOPY

Substrate for the histochemical demonstration of acid and alkaline phosphatase. Substrate for the fluorometric assay of acid and alkaline phosphatases. Demonstration of acid phosphatase in paraffin embedded tissue. Cytofluorometric assay of acid phosphatase in single living cells.

Supplier: SIGMA ALDRICH MICROSCOPY

Fast Blue BB Salt hemi (zinc chloride) salt, also known as Cl Azoic diazo 20 (Cl 37175) is a diazonium salt that possesses a lipophilic amine lipophilic with an aromatic system of moderate size. It is commercially available as the zinc chloride double salt. It has been used for alkaline phosphatase staining in cells.

Supplier: SIGMA ALDRICH MICROSCOPY

Toluidine blue (C.l. 52040) is a diaminothiazine dye. It is also known as TBO, tolonium chloride, and toluidine blue O.

Supplier: SIGMA ALDRICH MICROSCOPY

Giemsa stain is a versatile differential stain that is widely used in hematology for blood and bone marrow specimens, clinical cytology specimens, bacteriology, histological biopsies, and tumor samples. Giemsa's staining solution is composed of methylene blue, azure, and eosin. It is a major histological stain due to its unique staining of chromatin, nuclear membranes, and cytoplasmic elements. The stain is frequently used in combination with other dye solutions: May-Grünwald’s solution for Pappenheim (MGG) and Wright-Giemsa.

Supplier: SIGMA ALDRICH MICROSCOPY

Trypan blue is a tetrasulfonated anionic, hydrophilic azo dye derived from toluidine. The non-permeable cell membrane dye is a vital dye that selectively stains dead or non-viable cells, that pass through damaged but not through intact cell membranes.

Supplier: SIGMA ALDRICH MICROSCOPY

Density gradient medium is suitable for cell and subcellular organelle isolation, virus purification, and isolation of lipoproteins and macromolecules.

Supplier: SIGMA ALDRICH MICROSCOPY

Saffron, Sigma-Aldrich®

Supplier: SIGMA ALDRICH MICROSCOPY

Chief ingredient of Giemsa Stain.

Supplier: SIGMA ALDRICH MICROSCOPY

Alcian Blue 8GX is used as a heteroglycan stain for neutral, sulfated and phosphated mucopolysaccharides and glycosaminoglycans in tissues such as cartilage and extracellular matrices.

Supplier: SIGMA ALDRICH MICROSCOPY

Picric acid 13 g/l saturated solution in water, Sigma-Aldrich®

Supplier: SIGMA ALDRICH MICROSCOPY

Fast green FCF, Sigma-Aldrich®

Supplier: SIGMA ALDRICH MICROSCOPY

CC/mount is an aqueous based permanent mounting medium with a very high refractive index, which when applied to the stained tissue sections can store the tissue specimens permanently without the fading of the chromogens. Because of the superior refractive index of CC/mount, tissues mounted in this medium look like specimens mounted with organic based mounting media. No coverslipping is required with CC/mount. However, if coverslipping is required, dried CC/mount can be post mounted by using an organic based mounting medium. CC/mount permits the long-term storage of antigens localised using organic insoluble chromogen substrates, including AEC, DAB, Fast Red, BCIP/NBT, BCIP/INT and fluorescent dyes like FITC and phycobiliproteins. The high pH of CC/mount ensures increased stability of fluorescence intensity.

Supplier: SIGMA ALDRICH MICROSCOPY

ACCUSPIN System-Histopaque-1077 products are intended for use in the isolation of lymphocytes and other mononuclear cells. The separation medium, Histopaque-1077, is a sterile-filtered, endotoxin-tested solution of polysucrose and sodium diatrizoate, adjusted to a density of 1,077 g/ml. ACCUSPIN System-HISTOPAQUE-1077 employs γ-irradiated 50 mL polypropylene centrifuge tubes specially designed with two chambers separated by a porous high-density polyethylene barrier ('frit'). Each tube is aseptically filled with 15 ml of Histopaque 1077. Each tube will separate 15 to 30 ml of anticoagulated blood.

Supplier: SIGMA ALDRICH MICROSCOPY

Alcian Blue solution has been used in the histological staining of acidic glycosaminoglycans (GAGs; mucopolysaccharides) that are carboxylated or sulfated. Sulfated glycosaminoglycans appear to be the preferred substrate for alcian blue at pH 2.5. Alcian blue has been used to stain hyaluronan (HA), a nonsulfated GAG, but the intensity of the staining of HA is affected by the method of tissue fixation used. Alcian blue staining of GAGs in tissue may be modified through the addition of an electrolyte such as magnesium chloride.

Supplier: SIGMA ALDRICH MICROSCOPY

A preweighed vial containing 50 mg o-Dianisidine. o-Dianisidine (3,3′-dimethoxybenzidine) is a peroxidase substrate suitable for use in ELISA procedures. This substrate produces a soluble end product that is yellow-orange in color and can be read spectrophotometrically at 405 nm. The reaction may be stopped with 5 M HCl.

Supplier: SIGMA ALDRICH MICROSCOPY

Methyl green is a synthetic, triphenylmethane-type cationic dye related to ethyl green mainly used as DNA stain in histochemistry. It possesses aniline rings with different degrees of methylation. The dye binds specifically to the A-T rich regions of the major groove of DNA with the help of two positive charges. Methyl green in combination with pyronin is extensively used to stain RNA and plasma cells in tissue sections and cytologic preparations.