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28484 Results for: "G-Biosciences"

Accessories for protein kits

Accessories for protein kits

Supplier: G-Biosciences

LongLife™ Zymolyase®, 1,5 U/µl

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Water, Molecular Grade DNase-, RNase-free

Water, Molecular Grade DNase-, RNase-free

Supplier: G-Biosciences

Molecular Grade Water is ultrapure water that certified RNase, DNase and Protease free, is suitable for use in molecular biology applications, which demand a high quality of water and assurance that the water is free from nucleases and protease contamination. No toxic agents, such as DEPC, are used in the manufacturing of Molecular Grade Water, eliminating DEPC interference of enzymatic reactions.

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Sodium azide 1% in aqueous solution

Supplier: G-Biosciences

Sodium azide 1% in aqueous solution

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Water, sterile, distilled

Supplier: G-Biosciences

Laboratory reagent grade, sterile distilled water for your laboratory needs. This water is purified by steam distillation and sterile filtered for use as a research laboratory reagent. The distilled water is low endotoxin <0.025EU/ml and low conductivity (<10uS). This water is ideal for the preparation of reagents, rinsing of glassware and plasticware, and various other laboratory and research applications. No toxic agents such as DEPC are included in the preparation of distilled water, eliminating interference with enzymatic analysis.

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Phosphate buffered saline (PBS), 10X

Phosphate buffered saline (PBS), 10X

Supplier: G-Biosciences

PBS 10X solution is used as a wash buffers for Western blotting, ELISA and other applications.

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Experimentation kits, bacterial gram staining

Experimentation kits, bacterial gram staining

Supplier: G-Biosciences

The Gram staining method was first described in 1844 by the Danish bacteriologist Hans Christian Gram, after whom the test was named. The Gram staining test for bacteria is one of the most important tests in microbiology and is often one of the first tests performed in the identification of bacteria. The Gram staining method utilises the properties of bacterial cell walls and the stain crystal violet. Gram-positive microorganisms have a higher peptidoglycan and lower lipid content than Gram-negative bacteria in their cell walls and this makes them capable of retaining the Gram stain. This kit is supplied with two strains of bacteria and all the necessary components to carry out the Gram staining.

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Buffer, TBS solution 10X concentrate (TRIS buffered saline)

Supplier: G-Biosciences

The TBS solution is primarily used as a wash and blocking buffer diluent for Western blot, ELISA and immunoassays.

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Buffer, PBS solution 10X concentrate (phosphate buffered saline)

Supplier: G-Biosciences

10X PBS (Phosphate Buffered Saline) is a 10 fold concentrated, sterile, pH adjusted blend of phosphate buffer and saline solution, which when diluted 1 to 10 in ultrapure water produces a phosphate buffered saline of 8mM NaH2PO4, 150mM NaCl, 3mM KCl, and 2mM KH2PO4 at pH 7.4.

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Empty FPLC columns, G-TRAP™

Empty FPLC columns, G-TRAP™

Supplier: G-Biosciences

These empty columns are designed for fast protein liquid chromatography.

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Sodium chloride 0.9% in water normal saline solution

Supplier: G-Biosciences

G-Biosciences offers 0.9% NaCl in two convenient sizes to meet your particular laboratory needs. These sterile saline solutions are suitable for numerous applications.

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3-[(3-Cholamidopropyl)dimethylammonio]-1-propane sulphate (CHAPS)

Supplier: G-Biosciences

3-[(3-Cholamidopropyl)dimethylammonio]-1-propane sulphate (CHAPS)

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Lysates, mammalian GenLysate™, mouse

Lysates, mammalian GenLysate™, mouse

Supplier: G-Biosciences

GenLysates™ are total protein tissue and cell lysates that are extracted from the tissue or cell of interest. To inhibit proteolysis, the GenLysates™ are prepared in a lysis buffer containing a complete protease inhibitor cocktail. Each lysate lot is tested by electrophoresis and Western blot analysis.

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CytoScan™ Cell proliferation assay kit, WST-1

CytoScan™ Cell proliferation assay kit, WST-1

Supplier: G-Biosciences

CytoScan™ WST-1 Cell Proliferation Assay principle is based upon the reduction of the tetrazolium salt WST-1 to formazan by cellular dehydrogenases. The generation of the dark yellow coloured formazan is measured at 420 to 480 nm (optimal at 440 nm) and is directly correlated to cell number.

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Lysates, mammalian GenLysate™, macaque (primate)

Lysates, mammalian GenLysate™, macaque (primate)

Supplier: G-Biosciences

GenLysates™ are total protein tissue and cell lysates that are extracted from the tissue or cell of interest. To inhibit proteolysis, the GenLysates™ are prepared in a lysis buffer containing a complete protease inhibitor cocktail. Each lysate lot is tested by electrophoresis and Western blot analysis.

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Protease inhibitor cocktail, Mammalian ProteaseArrest™

Protease inhibitor cocktail, Mammalian ProteaseArrest™

Supplier: G-Biosciences

A broad range, 100X concentrated, ready-to-use protease inhibitor cocktail, Mammalian ProteaseArrest™ inhibits mammalian serine, cysteine and other mammalian specific proteases including aminopeptidases, trypsin-like and aspartic proteases.

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Sodium dodecyl sulphate (SDS)

Supplier: G-Biosciences

Sodium dodecyl sulphate (SDS)

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GET™ Plasmid DNA kits

GET™ Plasmid DNA kits

Supplier: G-Biosciences

GET™ Plasmid DNA Miniprep Kit isolates high quality plasmid DNA from 1 to 5 ml E. coli cultures. The kit utilises an enhanced DNA binding column to produce high yields of plasmid.

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Lysates, insect GenLysate™, Drosophila

Lysates, insect GenLysate™, Drosophila

Supplier: G-Biosciences

GenLysates™ are total protein tissue and cell lysates that are extracted from the tissue or cell of interest. To inhibit proteolysis, the GenLysates™ are prepared in a lysis buffer containing a complete protease inhibitor cocktail. Each lysate lot is tested by electrophoresis and Western blot analysis.

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EZ Yeast™ Plasmid Prep kit

EZ Yeast™ Plasmid Prep kit

Supplier: G-Biosciences

The EZ Yeast™ Plasmid Prep Kit is designed for the rapid isolation of plasmids from yeast patches or yeast grown in small liquid cultures. The kit is adapted from the alkaline lysis of E. coli, by providing modified alkaline lysis buffers and LongLife™ Zymolyase®, a highly stable enzyme.

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Biotin reagents, OneQuant™ HOOK™

Supplier: G-Biosciences

The OneQuant™ format prevents loss of reagent due to repeated weighing as each vial contains only 1 - 2 mg HOOK™ Biotin Reagent.

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femtoCHROMO™- HRP Substrate for chromogenic detection

femtoCHROMO™- HRP Substrate for chromogenic detection

Supplier: G-Biosciences

A ready to use modified TMB (3, 3’, 5, 5’-Tetramethylbenzidine) substrate is used that generates a dark blue precipitate in the presence of horseradish peroxidase.

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Genomic DNA isolation from fungi, OmniPrep™ for fungi kit

Genomic DNA isolation from fungi, OmniPrep™ for fungi kit

Supplier: G-Biosciences

OmniPrep™ for Fungi Kit isolates high quality genomic DNA from fungal samples.If used according to the protocols this kit purifies DNA from 1 to 2 g fungal tissues.

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Iodoacetamide

Supplier: G-Biosciences

Iodoacetamide

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Guanidinium hydrochloride 8 mol/l in aqueous solution

Supplier: G-Biosciences

A selection of strong chemicals to aid in the denaturation of proteins. Denaturants, or chaotropes, disrupt water interactions resulting in the solubilization of hydrophobic proteins and peptides. The chaotropes also act on all proteins to unfold them and alter their three-dimensional structure.

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Anti-IgG Rabbit Antibody (AP (Alkaline Phosphatase))

Supplier: G-Biosciences

Anti-IgG Rabbit Antibody (AP (Alkaline Phosphatase))

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(S)-(+)-Camptothecin 2 mM

(S)-(+)-Camptothecin 2 mM

Supplier: G-Biosciences

Apoptosis is commonly defined as programmed mechanism of cell death and is a highly regulated process that can be initiated by a variety of internal and external stimuli (inducers).

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Sulpho-SMPB (Sulphosuccinimidyl 4-(4-maleimidophenyl)butyrate)

Supplier: G-Biosciences

Sulfo succinimidyl 4-(ρ-maleimidophenyl) butyrate

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Carbohydrate reactive biotin reagents, HOOK™

Supplier: G-Biosciences

HOOK™ Biotin-Hydrazide and its long spacer arm equivalent, HOOK™ Biotin-LC-Hydrazide, are carbohydrate reactive reagents. HOOK™ carbohydrate reactive biotin reagents react with oxidised carbohydrate side chains. Some biotin reagents do not bind directly to the protein itself, but instead, conjugate to the carbohydrate residues of glycoproteins. The carbohydrate reactive biotin reagents contain hydrazides (-NH-NH₂) as a reactive group. The hydrazide reactions require carbonyl groups, such as aldehydes and ketones, which are formed by oxidative treatment of the carbohydrates. Hydrazides react spontaneously with carbonyl groups, forming a stable hydrazone bond. These reagents are particularly suitable for labelling and studying glycosylated proteins, such as antibodies and receptors.

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Dimethyl sulphoxide, anhydrous

Supplier: G-Biosciences

Anydrous DMSO [Dimethyl sulfoxide (CH3)2SO] is offered for the solubilization of the water insoluble cross-linkers and modification reagents.

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Gel loading dyes, Glow™

Gel loading dyes, Glow™

Supplier: G-Biosciences

These premixed and ready-to-use gel dyes are designed to minimise the use of ethidium bromide. Glow dyes provide intense DNA/RNA bands with little background or band distortion on any type of agarose or acrylamide gel.

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