Y-27632, Millipore®

A highly potent, cell-permeable, reversible, and selective inhibitor of Rho-associated protein kinases (Ki = 140 nm for p160ROCK). Also inhibits ROCK-II with equal potency. Also acts as a potent inhibitor of agonist-induced Ca²⁺ sensitization of myosin phosphorylation and smooth muscle contraction.

• Inhibition is competitive with respect to ATP
• Exhibits 10- to 50-fold lower affinity for PKCε than p160ROCK
• Pretreatment with Y-27632 reduces the risk of lung injury in rat models of sepsis
• Does not affect the activity of p21-activated protein kinase (PAK) even at higher concentrations (~100 µm)

Prevents apoptosis and enhances the survival and cloning efficiency of dissociated hES cells without affecting their pluripotency. A 5 mm (500 µg/296 µl) solution of Y-27632 in H2O and a 10 mm solution in DMSO are also available.