The NEXTFLEX® Unique dual index barcodes are barcoded adapters that can be used with single or paired-end read sequencing library prep kits designed for sequencing on Illumina® platforms workflows.

• 384 UDI adapters for mitigating sample misassignment due to index hopping Unique dual index adapters for multiplexing up to 384 libraries for Illumina® sequencing platforms Mitigate index hopping or spread of signal that can occur during sequencing Each lot of the NEXTFLEX® Unique dual index barcodes is tested for index purity by sequencing Compatible with both paired-end and single-read sequencing Compatible with the NEXTFLEX® Universal blockers

The NEXTFLEX® Unique dual index barcodes utilize two unique 8 nt indexes on each UDI adapter. The 8 nt indices are designed with a Hamming Distance of at least three throughout the entire series, permitting error correction. Patterned flow cells, such as those found on the Illumina® NovaSeq® and HiSeq® 3000/4000 platforms have been recognized to suffer from increased rates of sample mis-assignment during sequencing.

The use of adapters with unique dual indices during sequencing prevents such mis-assigned reads from appearing in final data sets, allowing for the highest assurance of data integrity. The NEXTFLEX® Unique dual index barcodes are barcoded adapters, for sequencing on Illumina® platforms, that provide data security in sequencing applications. Multiplexing with NEXTFLEX® Unique dual index barcodes increase scale while reducing costs by allowing the user to pool up to 384 libraries in a single flow cell lane.