The NEXTFLEX® RiboNaut™ rRNA depletion kit (HMR) effectively removes rRNAs from mammalian total RNA while enabling labs to find additional RNA species in a sample, not only limited to intact mRNAs.
- Hybridization and bead-based protocol for rRNA depletion Optimised for use with 5 ng to 1 µg of total RNA as starting material Versions are available for automated and manual rRNA depletion
The NEXTFLEX® RiboNaut™ rRNA depletion kit (Human/mouse/rat) is designed to be compatible with human, mouse, and rat total RNA. The method used in the NEXTFLEX® RiboNaut™ rRNA depletion kit is based on subtractive hybridisation, using a mixture of biotinylated oligonucleotides complementary to rRNAs.
After hybridisation of the bait oligos to total RNA, the rRNA/bait complex and excess bait oligos are removed by capture onto streptavidin-coated magnetic beads. The remaining RNA, which has been depleted of both cytoplasmic and mitochondrial rRNAs, is then purified and concentrated using magnetic beads.