Phusion® Hot Start II High-Fidelity DNA Polymerase allows the use of a wide range of primers, including those with low melting temperatures. Phusion® Hot Start II High-Fidelity DNA Polymerase is ideal for PCR applications from routine PCR to highly demanding applications, such as cloning and high throughput PCR. It incorporates a unique dsDNA-binding domain making this Pyrococcus-like proofreading DNA polymerase extremely robust, rapid, and accurate.

• No annealing temperature limitations
• Allows reaction set up at room temperature
• Zero-time reactivation due to unique Hot Start technology
• Fast PCR enzyme with extreme fidelity (over 6 times more accurate than Pfu)
• Robust enzyme with high tolerance against PCR inhibitors requires minimal optimisation