Corning® Matrigel® Basement Membrane Matrix, Corning
Growth Factor Reduced (GFR) formulation is well suited for applications that require a more highly defined basement membrane preparation.
- Mouse colonies routinely screened for pathogens via Mouse Antibody Production (MAP) testing
- Extensive PCR testing is performed to screen for a number of pathogens
- Tested and found negative for bacteria, fungi, and mycoplasma
- Protein concentrations determined by Lowry method
- Endotoxin units measured by Limulus Amoebocyte Lysate assay
- Matrigel Matrix gel stability tested for a period of 14 days at 37°C
- Biological activity determined for each lot using a neurite outgrowth assay.
Corning Matrigel matrix is a solubilized basement membrane preparation extracted from the Engelbreth-Holm-Swarm (EHS) mouse sarcoma, a tumor rich in extracellular matrix proteins, including Laminin (a major component), Collagen IV, heparin sulfate proteoglycans, entactin/nidogen, and a number of growth factors.
Application
• Cell Growth and Differentiation
• Metabolism/Toxicology Studies
• Invasion Assays
• In Vitro and In Vivo Angiogenesis Assays
• In Vivo Angiogenesis Studies and Augmentation of Tumors in Immunosuppressed Mice
Preparation and Storage
Store at -20°C. Avoid multiple freeze-thaws
Note: For research use only. Not intended for diagnostic or therapeutic procedures.
When warmed, the solution polymerizes to produce a biologically active matrix material resembling the mammalian cellular basement membrane. It is ideal for use with in vitro cell cultures of a broad range of cell types, including both primary and stem cell applications.
Growth Factor Reduced (GFR) formulation is well suited for applications that require a more highly defined basement membrane preparation.
- Mouse colonies routinely screened for pathogens via Mouse Antibody Production (MAP) testing
- Extensive PCR testing is performed to screen for a number of pathogens
- Tested and found negative for bacteria, fungi, and mycoplasma
- Protein concentrations determined by Lowry method
- Endotoxin units measured by Limulus Amoebocyte Lysate assay
- Matrigel Matrix gel stability tested for a period of 14 days at 37°C
- Biological activity determined for each lot using a neurite outgrowth assay.
Corning Matrigel matrix is a solubilized basement membrane preparation extracted from the Engelbreth-Holm-Swarm (EHS) mouse sarcoma, a tumor rich in extracellular matrix proteins, including Laminin (a major component), Collagen IV, heparin sulfate proteoglycans, entactin/nidogen, and a number of growth factors.
Application
• Cell Growth and Differentiation
• Metabolism/Toxicology Studies
• Invasion Assays
• In Vitro and In Vivo Angiogenesis Assays
• In Vivo Angiogenesis Studies and Augmentation of Tumors in Immunosuppressed Mice
Preparation and Storage
Store at -20°C. Avoid multiple freeze-thaws
Note: For research use only. Not intended for diagnostic or therapeutic procedures.
When warmed, the solution polymerizes to produce a biologically active matrix material resembling the mammalian cellular basement membrane. It is ideal for use with in vitro cell cultures of a broad range of cell types, including both primary and stem cell applications.
Documents
Cellular Analysis of 3D Spheroid-Based Tumor Invasion Assays Corning® Matrigel® Basement Membrane, Matrix for 3D Culture In Vitro Corning® Matrigel® Matrix, Frequently Asked Questions Culture and Assay Systems Used for 3D Cell Culture Methods for Implantation of Corning® Matrigel® Matrix into Mice and Tissue Fixation
- Catalog No:
- 734-0270
- 734-1100
- 734-0268
- 734-0269
- Environmentally Preferable:
- Low Manufacturing Impact
- Low Manufacturing Impact
- Low Manufacturing Impact
- Low Manufacturing Impact
- Quality control:
- Tested for ability to gel quickly and maintain this form with culture medium for a period of 14 days at 37 °C
Tested for the ability to promote neurite outgrowth of chick dorsal root ganglia cells
Tested and found negative for bacteria, fungi, and mycoplasma
Tested for endotoxin (LAL assay) - Tested for ability to gel quickly and maintain this form with culture medium for a period of 14 days at 37 °C
Tested for the ability to promote neurite outgrowth of chick dorsal root ganglia cells
Tested and found negative for bacteria, fungi, and mycoplasma
Tested for endotoxin (LAL assay)
- Source:
- Engelbreth-Holm-Swarm mouse tumour
- Engelbreth-Holm-Swarm mouse tumour
- Storage and stability:
- Stable for at least three months at −20 °C or 12 days at 37 °C
- Stable for at least three months at −20 °C or 12 days at 37 °C
- Environmental certification:
- ISO 14001 - Bedford
- ISO 14001 - Bedford
- ISO 14001 - Bedford
- ISO 14001 - Bedford
Product Family Options
- DescriptionPk
- Corning® Matrigel® Basement Membrane Matrix5 mL1 * 5 mL6 650,00 kr
Low Manufacturing Impact Specifications
Cat. No.734-0270Environmentally PreferableLow Manufacturing ImpactQuality controlSourceStorage and stabilityEnvironmental certificationISO 14001 - Bedford - Corning® Matrigel® Basement Membrane Matrix10 mL1 * 10 mL7 700,00 kr
Low Manufacturing Impact Specifications
Cat. No.734-1100Environmentally PreferableLow Manufacturing ImpactQuality controlTested for ability to gel quickly and maintain this form with culture medium for a period of 14 days at 37 °C
Tested for the ability to promote neurite outgrowth of chick dorsal root ganglia cells
Tested and found negative for bacteria, fungi, and mycoplasma
Tested for endotoxin (LAL assay)SourceEngelbreth-Holm-Swarm mouse tumourStorage and stabilityStable for at least three months at −20 °C or 12 days at 37 °CEnvironmental certificationISO 14001 - Bedford - Corning® Matrigel® Basement Membrane Matrix, growth factor reduced (GFR)5 mL1 * 5 mL7 750,00 kr
Low Manufacturing Impact Specifications
Cat. No.734-0268Environmentally PreferableLow Manufacturing ImpactQuality controlSourceStorage and stabilityEnvironmental certificationISO 14001 - Bedford - Corning® Matrigel® Basement Membrane Matrix, growth factor reduced (GFR)10 mL1 * 10 mL9 650,00 kr
Low Manufacturing Impact Specifications
Cat. No.734-0269Environmentally PreferableLow Manufacturing ImpactQuality controlTested for ability to gel quickly and maintain this form with culture medium for a period of 14 days at 37 °C
Tested for the ability to promote neurite outgrowth of chick dorsal root ganglia cells
Tested and found negative for bacteria, fungi, and mycoplasma
Tested for endotoxin (LAL assay)SourceEngelbreth-Holm-Swarm mouse tumourStorage and stabilityStable for at least three months at −20 °C or 12 days at 37 °CEnvironmental certificationISO 14001 - Bedford
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- DescriptionModelResolutionPackedPore sizeRecommended working volumeCulture areaMembrane ØMembraneNo. of wells
- 734-276396Half area microplate, with lid, tissue culture treated+200 µlBlack/clear1 * 16 Items - 5 550,00 kr
- 392-060896MicroWell™ plate, flat bottom, Nunclon™ Sphera™ treated surface+400 µlClear1 * 8 Items - 2 475,00 kr
- 738-020496Round bottom, spheroid with lid, ultra-low attachment surface+300 µlBlack/clear1 * 5 Items - 3 125,00 kr
- 734-2967Individually wrapped43 cm²Standard dish, with 1× surface treated scaffold insert (32,0×1,6 mm)011 * 40 Items - 5 850,00 kr
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Catalog #: 521-2636
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