Incucyte® Annexin V dye for apoptosis is a specially formulated, highly-selective phosphatidylserine (PS) cyanine fluorescent dye, designed for real-time evaluation of apoptosis in living cultures.

• Detect apoptosis using a highly-selective phosphatidylserine cyanine fluorescent dye
• Track relevant biology with a dye that is non-perturbing to cell health and morphology
• Reduce loss of precious or compromised cells under-going apoptosis with mix-and-read reagents

Application: Apoptosis/cell death quantification and immune cell killing.

One vial of lyophilised solid sufficient for 100 to 200 live-cell apoptosis tests (1 test = 1 well of 96-well microtiter plate) in 96-well format.

Addition of the Incucyte® Annexin V Dyes to normal healthy cells is non-perturbing to cell growth or morphology and yields little or no intrinsic fluorescent signal. Once cells become apoptotic, plasma membrane phosphatidylserine (PS) asymmetry is lost leading to exposure of PS to the extracellular surface and binding of the Incucyte® Annexin V Dye, yielding a bright and photostable fluorescent signal.

Once solubilised, the solution should be stored at +4 °C and protected from light. The reconstituted solution is stable for one week.