Pan-cancer 6-fusion panel (negative control) FFPE RNA reference standard is a highly-characterised, biologically-relevant quality control material used to assess the performance of targeted NGS RNA-seq, endpoint reverse-transcription PCR (RT-PCR), RT-qPCR and RT droplet digital PCR (RT-ddPCR) assays aimed at detecting gene fusions.

• Format: FFPE
• Fixation method: 10% formalin

This control was specifically designed to compliment pan-cancer 6-fusion panel.

Each FFPE section is composed of a formalin-fixed, paraffin-embedded (FFPE) cell-line verified not to contain TPM3-NTRK1, QKI-NTRK2, ETV6-NTRK3, EML4-ALK, CCDC6-RET and SLC34A2-ROS1 fusions.

Defined cell-line derived FFPE control material, verified as negative for TPM3-NTRK1, QKI-NTRK2, ETV6-NTRK3, EML4-ALK, CCDC6-RET and SLC34A2-ROS1 fusions.

Quality control:
Extractable RNA/expected yeild: ≥100 ng RNA per section using the Maxwell RSC® RNA FFPE purification kit.
RNA quantification: Qubit® RNA HS assay.
Fusion biomarker expression: Absence of TPM3-NTRK1, QKI-NTRK2, ETV6-NTRK3, EML4-ALK, CCDC6-RET and SLC34A2-ROS1 RNA transcripts confirmed by fusion specific RT-ddPCR using the QX200 ddPCR system.
RNA integrity: Expected DV200 ≥65% assessed using tapestation high sensitivity RNA screentape assay.