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Rat Cardiac Troponin I ELISA Kit
Rat Cardiac Troponin I ELISA Kit
Catalog # ANTIA74069-96
Leverantör:  ANTIBODIES.COM
New Product
CAS-nummer:  
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Rat Cardiac Troponin I ELISA Kit
Catalog # ANTIA74069-96
Leverantör:  ANTIBODIES.COM
New Product
CAS-nummer:  

Specifikationer

  • Förp.:
    96 Tests
  • Analysens varaktighet:
    Multiple steps
  • Analystyp:
    Sandwich
  • Formatera:
    Pre-coated
  • Primary antibody reactivity:
    Rat
  • Target protein:
    Cardiac Troponin I
  • Beskrivning:
    Rat cardiac troponin I ELISA kit
  • Typ av prov:
    Serum, plasma, tissue homogenates and other biological fluids
  • Korsreaktivitet:
    Rat Cardiac Troponin I ELISA Kit exhibits high specificity and excellent specificity for the detection of rat Cardiac Troponin I. No significant cross-reactivity or interference between Cardiac Troponin I and analogues was observed.
  • Detektionsmetod:
    Colorimetric
  • Time to Results:
    4 h 30 min
  • Analysprincip:
    Quantitative
  • Hållbarhetstid:
    Store for 6 months at 4 °C
  • Detektionsområde:
    31,25 - 2000 pg/ml
  • Förvaringstemperatur:
    4 °C
  • Provvolym:
    100 μl
  • Känslighet:
    18,75 pg/ml
  • Regulatory status:
    RUO

Specifikationer

Om denna produkt

Rat Cardiac Troponin I ELISA kit is a sandwich Enzyme-Linked Immunosorbent Assay (sELISA) designed for the in vitro quantitative determination of rat Cardiac Troponin I in serum, plasma, tissue homogenates, and other biological fluids.

  • Ready-to-use ELISA kit
  • Detection Range: 31,25 to 2000 pg/ml
  • Sensitivity: 18,75 pg/ml
  • Sample Type: Serum, plasma, tissue homogenates, and other biological fluids
  • Assay Precision: Intra-Assay: CV <8%, Inter-Assay: CV <10%

Rat Cardiac Troponin I ELISA Kit employs the sandwich enzyme immunoassay technique for the quantitative measurement of rat Cardiac Troponin I in serum, plasma, tissue homogenates, and other biological fluids. An antibody specific for Cardiac Troponin I has been pre-coated onto a 96-well microtiter plate. The standards and test samples are added into the wells and the Cardiac Troponin I present in each sample is bound to the wells by the immobilised antibody. Following incubation, the wells are washed and then incubated with Biotinylated Anti-Cardiac Troponin I Antibody, which binds the captured Cardiac Troponin I present in each well. Following incubation, unbound biotinylated detection antibody is removed by washing, and an HRP-Streptavidin conjugate is added to the wells and the microtiter plate is incubated. Following incubation and washing, TMB substrate solution is then used to visualise the HRP enzymatic reaction by catalysis to produce a blue-coloured product that changes to yellow after addition of acidic stop solution. The density of yellow is proportional to the amount of Cardiac Troponin I captured in each well. The concentration of Cardiac Troponin I can then be calculated by reading the O.D. absorbance at 450 nm in a microplate reader and referring to the standard curve.

Ordering information: This is a ready-to-use ELISA kit that contains: Pre-coated 96 well microplate, lyophilised standard, sample dilution buffer, biotinylated detection antibody (100X), antibody dilution buffer, HRP-Streptavidin conjugate (SABC) (100X), SABC dilution buffer, TMB substrate, stop solution, wash buffer (25X), plate sealers, foil pouch, and protocol manual.