- Förp.:96 Tests
- Analysens varaktighet:Multiple steps
- Analystyp:Sandwich
- Formatera:Pre-coated
- Primary antibody reactivity:Mouse
- Target protein:ST2
- Beskrivning:Mouse ST2 ELISA kit
- Typ av prov:Serum, plasma, tissue homogenates, and other biological fluids
- Korsreaktivitet:Mouse ST2 ELISA Kit exhibits high specificity and excellent specificity for the detection of mouse ST2. No significant cross-reactivity or interference between ST2 and analogues was observed.
- Detektionsmetod:Colorimetric
- Time to Results:4 h 30 min
- Hållbarhetstid:Store for 6 months at 4 °C
- Detektionsområde:0,156 - 10 ng/ml
- Förvaringstemperatur:4 °C
- Känslighet:0,094 ng/ml
- Regulatory status:RUO
Mouse ST2 ELISA kit is a sandwich Enzyme-Linked Immunosorbent Assay (sELISA) designed for the in vitro quantitative determination of mouse ST2 in serum, plasma, tissue homogenates, and other biological fluids.
- Ready to use ELISA kit
- Assay precision: Intra assay: CV <8%, Inter assay: CV <10%
Mouse ST2 ELISA kit (A303603) employs the sandwich enzyme immunoassay technique for the quantitative measurement of mouse ST2 in serum, plasma, tissue homogenates, and other biological fluids. An antibody specific for ST2 has been pre-coated onto a 96-well microtiter plate. The standards and test samples are added into the wells and the ST2 present in each sample is bound to the wells by the immobilised antibody. Following incubation, the wells are washed and then incubated with Biotinylated Anti-ST2 Antibody, which binds the captured ST2 present in each well. Following incubation, unbound biotinylated detection antibody is removed by washing, and an HRP-Streptavidin conjugate is added to the wells and the microtiter plate is incubated. Following incubation and washing, TMB substrate solution is then used to visualise the HRP enzymatic reaction by catalysis to produce a blue-coloured product that changes to yellow after addition of acidic stop solution. The density of yellow is proportional to the amount of ST2 captured in each well. The concentration of ST2 can then be calculated by reading the O.D. absorbance at 450 nm in a microplate reader and referring to the standard curve.