Non-reactive mouse antibody that serves as a negative control for ExoBrite™ Flow antibodies when detecting purified or bead-bound EVs by flow cytometry.

• Non-reactive IgG1 mouse antibody
• Serves as a isotype negative control for ExoBrite™ Flow antibodies
• Available in 5 colours

ExoBrite™ IgG1 Isotype control flow antibody has been found to not react with any target in human cells and have the same isotype as the ExoBrite™ Flow antibodies against tetraspanin proteins CD9, CD63, and CD81. The antibody is available with the same conjugates as ExoBrite™ Flow antibodies for the pacific Blue™, FITC, PE, and APC channels.

Extracellular vesicles (EVs), including exosomes, are lipid-bound vesicles that are released from cells. EVs display specific surface proteins and can carry nucleic acids and other cargo, allowing them to transfer biological information between cells in different parts of the body. Therefore EVs are increasingly studied for their potential use in drug delivery and medical diagnostic applications. The most common proteins used as EV markers are CD9, CD63, and CD81, members of the tetraspanin family. Tetraspanins are plasma membrane proteins with many proposed functions, including activation and sorting of other membrane proteins. They are also thought to play a role in the targeting of proteins to multivesicular bodies (MVBs) and exosomes. These tetraspanins are broadly expressed on many cell types and can therefore be detected on many types of EVs, but their expression levels vary depending on the cell type of origin.

Type:
Antigen:
Clonality: Monoclonal
Clone:
Conjugation: ExoBrite 650/665
Epitope:
Host: Mouse
Isotype: IgG1
Reactivity: