In this experiment, transformed cells take up a plasmid containing the Green Fluorescent Protein (GFP) gene. The GFP gene was isolated from the jellyfish Aequorea victoria. Transformed colonies expressing the GFP protein are visibly green in normal light but will fluoresce brightly when exposed to longwave UV light.

• For 10 lab groups
• Plating 5 minutes; incubation overnight; transformation efficiency 15 minutes

Also required: 37 °C incubator, water bath, longwave UV lamp.