For label-free quantitation and kinetic analysis of his-tagged proteins.

• High specificity quantitation of his-tagged proteins
• Easy capture of his-tagged proteins for kinetic analysis with interacting protein analyte
• High specificity and High affinity towards polyhistidine tag
• Allows rapid analysis in purified or crude samples

The polyhistidine tag, commonly known as His-tag, is fused to recombinant proteins as a means of facilitating detection and purification. The dip and read anti-penta-HIS (HIS1K) biosensor consists of high affinity, high specificity penta-his antibody from QIAGEN pre-immobilized on a sartorius fibre optic biosensor.

In conjunction with the Octet® systems, the HIS1K biosensor provides a rapid and label-free method for his-tagged protein quantitation and kinetic analysis. The high specificity of the antibody-based biosensor enables the direct capture and quantitation of his-tagged proteins in crude lysates, column eluates, cell lysates and cell culture supernatants, serving as an alternative to traditional time consuming analytical methods such as HPLC and ELISA.