The simplicity and convenience of Mix-n-Stain™ labelling of nanobodies with fluorescent CF® dyes or biotin.

• Rapid, simple, covalent conjugation of Nanobodies® (also called camelid single variable or VHH domains)
• No purification required after labelling
• Reaction takes only 30 minutes, with minimal hands-on time
• Choice of seven next-generation CF® dyes with superior brightness and photostability, or biotin
• Compatible with common stabilizers

Mix-n-Stain™ Nanobody labelling kits allow to rapidly label 5 to 20 µg or 20 to 50 µg Nanobody® (also called camelid single variable or VHH domains) with one of Biotium's next-generation CF® dyes or biotin. Simply mix your Nanobody® with the reaction buffer and optimally formulated dye provided, followed by a 30 minute incubation and addition of the provided stop buffer. Any remaining free dye or other label is no longer reactive at the end of the labelling, so the conjugate is ready for staining without further purification. After the reaction, the Nanobody® will be labelled with an average of 1 to 2 dye molecules per Nanobody® molecule. Mix-n-Stain™ labelling is covalent, so labelled nanobodies can be used for multiplex staining without transfer of dye or biotin between proteins.

These kits feature a choice of Biotium’s next-generation CF® dyes, which were designed for advantages in brightness and photostability compared to Alexa Fluor®, DyLight®, and other fluorescent dyes. Biotium also offers kits for labelling IgG antibodies with CF® dyes and other labels.

Alexa Fluor and DyLight are registered trademarks of Thermo Fisher Scientific. Cy Dye is a registered trademark of GE Healthcare.