SF-4 baculo express insect medium

SF-4 baculovirus expression medium allows high yield expression of proteins well above all other commercially available media. The products has no variability therefore does not require initial batch testing and batch reservation.

• High Cell Density: Densities up to 2×10⁷ cells/ml can be achieved using SF-4 in bioreactors and spinner flasks
• Versatility: Not only suitable for SF9 and SF21 but also for High Five™ and Drosophila cells
• Fast adaptation: Only few passages are needed, if you switch from your current serum supplemented medium. Direct switch from your current serum free medium is possible for some of the commercially available media
• High Protein Yield: Results indicate an increased protein yield (1,5 to 2,7 times) in recombinant protein production compared to previously used media
• No variability between lots
• Stable in fridge for long periods of time
• Cost competitive
• High yield of expressed proteins beyond current commercial media

Extensive development and further investigation on the nutritional needs of insect cells, based on the excellent performance of SF-1, resulted in our improved 'ready to use' SF-4 baculo express medium. SF-4 baculo express medium is a proprietary serum free formulation which has successfully been used to grow various Spodoptera frugiperda (SF9, SF21), BTI-TN-5B1-4 (High FiveTM) and Drosophila melanogaster (D.Mel-2) cells. With SF-4 baculo express media all cell types can be cultivated: monolayer-, spinner- or shaking cultures, adherent cells and suspension cells. The medium contains all additives necessary for the cultivation of insect cells in the optimal concentration: steroids, amino acids, organic acids, glutamine and glucose. The medium is designed for efficient infection and expression with the Baculovirus systems.

Certifications: ISO 9001; cGMP.