Specifications

• Pk:
  1 mg
• Conjugation:
  Unconjugated
• Protein/peptide type:
  Recombinant
• Source:
  HEK293 cells
• Species:
  Human
• Tag sequence:
  His Tag (active trimer) (MALS verified)
• Storage conditions:
  –20 °C
• Endotoxin content:
  Less than 1,0 EU per μg by the LAL method.
• Biological activity:
  Anti-TNFSF11 (human IgG1) captured on CM5 chip via Anti-human IgG Fc antibodies surface can bind Human TNFSF11, His Tag (active trimer) (ACRBRAL-H5240) with an affinity constant of 0,274 nM as determined in a SPR assay (Biacore T200) (Routinely tested). Captured Human RANK, Mouse IgG2a Fc Tag, low endotoxin (ACRBRAK-H5251) on CM5 chip via Anti-Mouse antibodies surface can bind Human TNFSF11, His Tag (active trimer) (ACRBRAL-H5240) with an affinity constant of 0,309 nM as determined in a SPR assay (Biacore T200) (Routinely tested). Loaded Human RANK, Mouse IgG2a Fc Tag, low endotoxin (ACRBRAK-H5251) on AMC Biosensor, can bind Human TNFSF11, His Tag (active trimer) (ACRBRAL-H5240) with an affinity constant of 0.409 nM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested). Loaded Anti-TNFSF11 MAb (human IgG1) on AHC Biosensor, can bind Human TNFSF11, His Tag (active trimer) (ACRBRAL-H5240) with an affinity constant of 0.239 nM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).
• Gene ID:
  AAC51762.1
• Protein synonyms:
  OPGL|ODF|TRANCE|CD254|RANKL
• Protein/peptide name:
  TNFSF11
• Purity:
  >90% as determined by SDS-PAGE.
• Molecular weight:
  22,4 kDa
• Endotoxin level:
  Low
• Formulation:
  Lyophilised from 0,22 μm filtered solution in PBS, pH 7,4. Normally trehalose is added as protectant before lyophilisation.
• Shipping temperature:
  RT