Human recombinant siglec-15 (from HEK293 cells)

Human recombinant siglec-15 (from HEK293 cells)

Human Siglec-15 / CD33L3 Protein, His Tag.

Conjugation:
Unconjugated
Protein/peptide type:
Recombinant
Source:
HEK293 cells
Species:
Human
Tag sequence:
His Tag
Storage conditions:
–20 °C
Endotoxin content:
Less than 1,0 EU per μg by the LAL method
Biological activity:
Measured by its binding ability in a functional ELISA. Immobilised Human Siglec-15, His Tag (Cat. No. SG5-H52H3) at 5 μg/ml (100 μl/well) can bind Mouse Human chimeric MAb (5G12, Human IgG1) with a linear range of 0,3 - 2 ng/ml (QC tested). Measured by its binding ability in a functional ELISA. Immobilised Human Siglec-15, His Tag (Cat. No. SG5-H52H3) at 10 μg/ml (100 μl/well) on Nickel Coated plate, can bind Neu5Ac(a2-6)GalNAc-PAA-biotin with a linear range of 0.078-5 μg/ml (Routinely tested). Loaded Mouse Human chimeric MAb (5G12, Human IgG1) on AHC Biosensor, can bind Human Siglec-15, His Tag (Cat. No. SG5-H52H3) with an affinity constant of 0,52 nM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).
Gene ID:
Q6ZMC9-1
Protein synonyms:
Siglec-15|SIGLEC-15|Siglec15|CD33L3|CD33 antigen-like 3|sialic acid-binding Ig-like lectin 15
Protein/peptide name:
Siglec-15
Purity:
>90% as determined by SDS-PAGE.
Molecular weight:
28,4 kDa
Endotoxin level:
Low
Formulation:
Lyophilised from 0,22 μm filtered solution in 25 mM MES, 150 mM NaCl, pH 5,5. Normally trehalose is added as protectant before lyophilisation.
Shipping temperature:
RT