Human recombinant IL-12 R beta 1 (from HEK293 cells)
- Conjugation:Unconjugated
- Protein/peptide type:Recombinant
- Source:HEK293 cells
- Species:Human
- Tag sequence:Fc Tag
- Storage conditions:–20 °C
- Endotoxin content:Less than 1,0 EU per μg by the LAL method.
- Biological activity:Measured by its binding ability in a functional ELISA. Immobilised Human IL-12B, His Tag (Cat. No. NK2-H52H7) at 5 μg/ml (100 μl/well) can bind Human IL-12 R beta 1, Fc Tag (Cat. No. ILB-H5255) with a linear range of 5 - 78 ng/ml (QC tested). Human IL-12 R beta 1, Fc Tag (Cat. No. ILB-H5255) captured on CM5 chip via Anti-human IgG Fc antibodies surface can bind Human IL-12B&IL-12A Heterodimer Protein, His Tag&Flag Tag (Cat. No. IL2-H4210) with an affinity constant of 2,06 nM as determined in a SPR assay (Biacore T200) (Routinely tested). Loaded Human IL-12 R beta 1, Fc Tag (Cat. No. ILB-H5255) on Protein A Biosensor, can bind Human IL-12B, His Tag (Cat. No. NK2-H52H7) with an affinity constant of 7,14 nM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested). Loaded Human IL-12B&IL-12A Heterodimer Protein, His Tag&Flag Tag (Cat. No. IL2-H4210) on HIS1K Biosensor, can bind Human IL-12 R beta 1, Fc Tag (Cat. No. ILB-H5255) with an affinity constant of 32,3 nM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).
- Gene ID:P42701-1
- Protein synonyms:CD212|IL12RB|IL-12 R beta 1|IL12RB1|IL-12RB1|IL12R
- Protein/peptide name:IL-12 R beta 1
- Purity:>95% as determined by reduced SDS-PAGE.
- Molecular weight:83,5 kDa
- Endotoxin level:Low
- Formulation:Lyophilised from 0,22 μm filtered solution in Tris with Glycine, Arginine and NaCl, pH 7,5. Normally trehalose is added as protectant before lyophilisation.
- Shipping temperature:RT