Human Recombinant PDGF-AB (Animal free) (from E. coli)

Supplier: Shenandoah Biotechnology

Certificates

Supplier: Shenandoah Biotechnology

About this item

Platelet-derived growth factor (PDGF) is an important regulator of cell growth, proliferation, and angiogenesis. PDGF synthesis is induced by IL-1, IL-6, TNF-α, TGF-β and EGF signaling. PDGF functions as a mitogenic growth hormone on cells of mesenchymal lineage, such as smooth muscle and glial cells.

High quality
Low endotoxin
Affordable

PDGF is also stored in the alpha-granules of platelets and is released upon adherence to traumatized tissues. PDGF is a dimeric glycoprotein formed by two A chains (AA), two B chains (BB), or as a heterodimer with an A and a B chain (AB). The PDGF dimer binds the cell surface receptor tyrosine kinases PDGFR-α and PDGFR-β.

Delivery: Certificate of Analysis (COA) is supplied with each product shipped detailing purity, formulation, biological activity, endotoxin levels, reconstitution instructions and recommended storage conditions.

Caution: For research use only.

Specifications

Konjugation:
Unconjugated
Protein function:
Cytokine
Protein/peptide type:
Recombinant
Källa:
E. coli
Species:
Human
Storage conditions:
−20 °C
Endotoxiinnehåll:
Endotoxin LAL (EU/µg) ≤0.1
Biologisk aktivitet:
Bioactivity assay: NR6R-3T3 cell proliferation, ED50 ≤20 ng/ml, bioactivity ≥5,0×10⁴ units/mg
Gen-ID:
P04085/P01127
Reconstitution instructions:
Sterile water at 0,1 mg/ml
Endotoxinfri:
N
Bärarfri:
Y
Protease-free:
N
Djurfri:
Y
Protein synonyms:
ODGF|GDGF
UniProtKB:
P04085/P01127
Protein/peptide name:
PDGF-AB
Renhet:
≥95%
Molekylvikt:
Alpha: 14,4 kDa, beta: 12,4 kDa, total 26,8
Sequence:
Alpha chain: MSIEEAVPAV CKTRTVIYEI PRSQVDPTSA NFLIWPPCVE VKRCTGCCNT SSVKCQPSRV HHRSVKVAKV EYVRKKPKLK EVQVRLEEHL ECACATTSLN PDYREEDTGR PRESGKKRKR KRLKPT Beta chain: MSLGSLTIAE PAMIAECKTR TEVFEISRRL IDRTNANFLV WPPCVEVQRC SGCCNNRNVQ CRPTQVQLRP VQVRKIEIVR KKPIFKKATV TLEDHLACKC ETVAAARPVT
Endotoxinnivå:
Low
Formulering:
0,1% Trifluoroacetic Acid (TFA)
Fritt från nukleaser:
N
Klass:
RUO
Shipping temperature:
RT
Tested applications:
Bioassay