Allylamine-2’-deoxyuridine-5’-triphosphate (Allylamine-dUTP) can replace TTP in reactions where it serves as a substrate for E.coli DNA polymerase 1 (holoenzyme and Klenow fragment), terminal deoxynucloetide transferase, T4 and Taq DNA polymerases, and reverse transcriptases (from ALV and MLV).

Allylamine-dUTP is incorporated into DNA by a variety of labelling reactions including nick translation, random primed DNA synthesis and 3’ end labelling reactions. Allylamine-labelled DNA can be efficiently conjugated to the active ester form of signal generating moieties such as fluorescent dyes, biotin and other haptens to produce labelled probes for hybridisation/detection assays. The efficient incorporation of Allylamine-dUTP, in contrast to many dye-labelled nucleotides, provides for greater incorporation of signalling moieties and stronger signals.