VWR® Taq DNA Polymerase Glycerol Free 5U/µl is a thermostable recombinant DNA polymerase, which exhibits very high activity in primer extension and other molecular biology applications. The enzyme is isolated from Thermus aquaticus and has a molecular weight of approximately 94 kDa.

• Most suitable choice for routine applications where high fidelity is not required
• High performance, thermostable DNA polymerase
• Optimal for TA cloning

VWR® Taq DNA Polymerase Glycerol Free has both a 5'→3' DNA polymerase and a 5'→3' exonuclease activity. The enzyme lacks a 3'→5' exonuclease activity (no proofreading ability). VWR® Taq DNA Polymerase Glycerol Free leaves an A' overhang, which makes the enzyme ideal for TA cloning.

10X Key Buffer: Tris-HCL pH 8,5, (NH₄)₂SO₂, 15 mM MgCl₂, 1% Tween-20®

10X Extra Buffer: 100 mM Tris-HCL pH 8,3, 500 mM KCl, 15 mM MgCl₂, 1% Triton X-100

10X Mg-Free Key Buffer: Tris-HCL pH 8,5, (NH₄)₂SO₂, 1% Tween-20®

10X Mg-Free Extra Buffer: Tris-HCL pH 8,3, KCl, 1% Triton X-100