Klenow (3'-5' exo-) (from E. coli carrying the Klenow (3'-5' exo-) gene)
ENZOENZ44001U020
:
- Pk:20 µl
- Source:E. coli carrying the Klenow (3'-5' exo-) gene
- Seq:02
- Storage conditions:Stable for at least 1 year after receipt when stored at –20 °C
- Form:Liquid
- Shipping conditions:Dry Ice
- Enzyme name:Klenow (3'-5' exo-)
- Enzyme activity:10000 U/mg
- Purity:≥99% (SDS-PAGE)
- Formulation:In 20 mM TRIS-HCl, pH 7,5, containing 50% glycerol, 0,1 mM EDTA and 1 mM DTT.
Klenow (3′-5′ exo-) is a mesophilic DNA polymerase deficient in both proofreading (3′-5′) and nick-translation (5′-3′) nuclease activities, and that displays a moderate strand displacement activity during DNA synthesis. The protein is expressed as a truncated product of the E.coli PolA gene and contains the D355A and E357A mutations.
- Used for DNA labeling
- Not for 5´→3´ or 3´→5´exonuclease activity
- N-terminal truncated DNA Polymerase I which retains polymerase activity
It is formulated of liquid in 20 mM TRIS-HCl, pH 7,5, containing 50% glycerol, 0,1 mM EDTA and 1 mM DTT.