Rat FGF basic (from E. coli)
: Biorbyt
- Source:E. coli
- Species:Rat
- Storage conditions:Store at 4 °C for up to two weeks. For long term storage, aliquot and store at −20 °C, avoid freeze/thaw cycles.
- Biological activity:The ED50 range as determined by the dose-dependant proliferation of BALB/3T3 cells was found to be less than 0.2 ng/ml, corresponding to specific activity of 5,000,000 IU/mg.
- Protein synonyms:HBGH-2
- Protein/peptide name:FGF basic
- Purity:> 97.0% as determined by RP-HPLC and analysis by SDS-PAGE
- Solubility:It is recommended to reconstitute the lyophilized Rat bFGF in sterile H2O not less than 100ug/ml, which can then be further diluted to other aqueous solutions.
- Sequence:PALPEDGGGA FPPGHFKDPK RLYCKNGGFF LRIHPDGRVD GVREKSDPHV KLQLQAEERG VVSIKGVCAN RYLAMKEDGR LLASKCVTEE CFFFERLESN NYNTYRSRKY SSWYVALKRT GQYKLGSKTG PGQKAILFLP MSAKS.
- Formulation:FGF-b was lyophilized from mg/ml solution after extensive dialysis against 20 mM phosphate buffer, pH 7.4 and 130mM NaCl.
- Tested applications:SDS-PAGE , HPLC , FuncS
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