SuperSignal™ West Pico PLUS Chemiluminescent Substrate is an enhanced chemiluminescent (ECL) horseradish peroxidase (HRP) substrate that enables picogram- to high femtogram-level protein detection by Western blot analysis.
- Detect low-picogram to high-femtogram amounts of target protein on nitrocellulose or PVDF membrane
- Incubated blots provide stable signal duration over the critical 4 hour time period with up to 24 hours of light output under optimal conditions
- Stable reagent – 8-hour working solution stability; up to one year kit stability at room temperature
- Optimised for dilute antibody concentrations*
- Provides high performance outside of the recommended antibody dilution ranges, including the most common 1:5 K to 1:10 K secondary antibody dilutions of a 1 mg/ml stock solution
SuperSignal™ West Pico PLUS substrate is designed to provide excellent signal intensity and sensitivity for western blotting with HRP conjugates. The intensity of the light emission combined with the exceptional duration allows for the acquisition of multiple exposures to more easily obtain publication-quality blot images. SuperSignal™ West Pico PLUS substrate is compatible with different membranes, blocking reagents, and a wide range of antibody dilutions making it an ideal choice for most western blotting applications.
*0,2 to 1,0 µg/ml primary antibody (1:1000 to 1:5000 dilution from 1 mg/ml stock); 10 to 50 ng/ml secondary antibody (1:20,000 to 1:100,000 dilution from 1 mg/ml stock)