AminoLink™ Plus Coupling Resin uses aldehyde-activated agarose beads for high-yield covalent coupling of antibodies (proteins) via primary amines to prepare columns for affinity purification.

• AminoLink Plus CouplingResin - aldehyde-activated crosslinked 4% beaded agarose
• Ideal for antibodies and other proteins - immobilise molecules via primary amines (-NH2)
• Stable, permanent immobilisation - Coupling reaction esults in stable, leak-resistant secondary amine bond between resin and ligand
• Better than immobilisation to CNBr-activated agarose - bond is more stable and uncharged, resulting in less nonspecific binding in affinity purification procedures
• Versatile and reusable - prepared affinity resin is adaptable to column and batch affinity techniques and the resin is reusable for typical applications based on protein binding interactions

The activated support contains aldehyde functional groups that spontaneously react with primary amines on proteins or other molecules. The Schiff base bonds that form are reduced to stable secondary amine bonds in the presence of the mild reducing agent, sodium cyanoborohydride. Coupling efficiency by this reductive amination mechanism is typically greater than 80%, regardless of the ligand’s molecular weight or pI. The resin is crosslinked beaded agarose with fast linear flow potential, making it useful for gravity flow and low- to medium-pressure applications.

Flexible coupling conditions provide efficient (>85%) coupling over a wide range of pH (4 to 10) and buffer conditions (PBS or other non-amine buffer with or without organic solvent); regular (PBS, pH 7,2) and enhanced (citrate-carbonate, pH 10) coupling protocols provided.

AminoLink Plus Resin is beaded agarose support that has been modified to contain aldehyde groups. Antibodies, other proteins, or any molecules with primary amines can be conjugated to the resin by an optimized form of reductive amination. Once an antibody or other ligand is immobilised, the prepared affinity resin can be used for a variety of affinity purification methods involving batch or column chromatography. The resin and linkage are stable in binding and elution conditions typically used in affinity chromatography, enabling prepared resin to be used for at least 10 rounds of affinity purification.