Ubiquitin enrichment kit, Pierce™
Catalog # PIER89899
Supplier: Thermo Fisher Scientific
CAS Number:
Specifications
- Pk:1 KIT
- Description:Ubiquitin Enrichment Kit, sufficient for enriching polyubiquitinated proteins from 15 lysate samples each containing 0,15 mg total protein
Specifications
About this item
The Pierce™ Ubiquitin enrichment kit facilitates the isolation of polyubiquitin protein conjugates from cultured cells and tissue samples.
- Takes less than 45 minutes hands-on time for binding and recovery of ubiquitinated proteins
- Sample incubation from 2 hours to overnight allows assay to be completed in several hours or in less than 30 minutes after overnight incubation
- Compatible with all cell lysis products and standard RIPA formulations
- Easily processes 1 to 15 samples concurrently
The fractions recovered from this Ubiquitin enrichment kit can be subsequently analysed to determine the amount of general ubiquitin conjugates present or to identify a specific protein of interest by Western blotting. The assay protocol is fast and straightforward, allowing for isolation of polyubiquitinated proteins and the fractionation of monoubiquitinated species in the resin flow-through. It provides exceptional performance, as demonstrated by enriching an epoxomicin-treated HeLa lysate for poly ubiquitinated proteins.
Delivery: The kit contains polyubiquitin positive control (1000X, 50 µl), anti-ubiquitin antibody (rabbit antiserum, 5 µl), polyubiquitin affinity resin slurry (300 µl), TBS pack (makes 500 ml), and spin columns (18) and accessories.