Anti-AKT1 Mouse Monoclonal Antibody (Unconjugated) [clone: 14E5.A2.B2.H9]
Catalog # ROCK200-301-I51S
Supplier: Rockland Immunochemicals
Specifications
- Antibody type:Primary
- Antigen name:Protein Kinase B alpha
- Antigen symbol:PKBA
- Clonality:Monoclonal
- Clone:14E5.A2.B2.H9
- Conjugation:Unconjugated
- ELISA:Yes
- Flow cytometry:Yes
- Host:Mouse
- ImmunoChemistry:Yes
- Isotype:IgG2a kappa
- Reactivity:Human
- Western blot:Yes
- Epitope:internal
- Cross adsorption:No
- Antigen synonyms:AKT
- Storage buffer:0,02 M Potassium Phosphate, 0,15 M Sodium Chloride, pH 7,2, 0,01% (w/v) Sodium Azide
- Storage temperature:–20 °C
- Shipping temperature:Dry Ice
- Immunogen:Anti-AKT1 Antibody was produced by repeated immunizations with a synthetic peptide corresponding to internal residues of human AKT1 protein.
- Purification:Anti-AKT1 antibody is directed against human AKT1. The antibody detects both unphosphorylated and phosphorylated forms of the protein. Anti-AKT1 antibody was purified from ascites by Protein A chromatography. Cross reactivity with AKT1 from other species has not been determined, however, the sequence of the immunogen shows 85% identity to mouse and 92% identity with rat, therefore, cross reactivity is expected.
- Size:25 µl
- Pk:25 µl
Specifications
About this item
Anti-AKT1 Antibody is suitable for ELISA, immunohistochemistry, and western blotting. Expect a band approximately 56 kDa in size corresponding to AKT1 protein by western blotting in the appropriate cell lysate or extract.
Recommended Dilutions: ELISA: 1:2000 - 1:10000; Flow Cytometry: User Optimized; Western Blot: 1:1000; Immunochemistry: 20 µg/ml; Other Dilution: User Optimized
Type: Primary
Antigen: AKT1
Clonality: Monoclonal
Clone: 14E5.A2.B2.H9
Conjugation: Unconjugated
Epitope: Center
Host: Mouse
Isotype: IgG2a kappa
Reactivity: Human