Phusion® Green hot start II High-fidelity DNA polymerase
Supplier: FINNZYMES REAGENTS
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About this item
Phusion® Green Hot Start II High-Fidelity DNA Polymerase is a combination of Phusion® Hot Start II DNA Polymerase and 5× Phusion® Green buffers. The buffers include a density reagent and two tracking dyes for direct loading of PCR products on a gel. The coloured buffer does not interfere with excellent performance of Phusion® Hot Start II DNA polymerase and is compatible with downstream applications such as DNA sequencing, ligation and restriction digestion.
- Reaction set up can be done at room temperature
- Reduces non-specific amplification and prevents primer degradation during set up
- Zero-time reactivation due to hot start technology
- Speed of the polymerase allows short extension times
- Robust reactions, minimal optimisation needed
- Increased product yields with minimal enzyme amounts
- Direct loading on gel with Phusion® Green buffer
Delivery: Kit contains Phusion® Hot Start II High-Fidelity DNA Polymerase (two U/µl), 5X Phusion® Green HF Buffer, 5X Phusion® Green GC Buffer, DMSO and 50 mM MgCl₂. Both Phusion® and Phusion® Green buffers provide 1,5 mm MgCl₂ in the final 1× concentration.