Multimode columns, HiTrap™ Capto™ MMC

HiTrap Capto MMC is prepacked with Capto MMC, a multimodal BioProcess ion exchange resin. The multimodal functionality gives a different selectivity compared with traditional ion exchangers and it binds proteins at high or low ionic strength.

• High dynamic binding capacity at high conductivity
• Selectivity allows binding in the presence of salt
• High volume throughput
• HiTrap Capto MMC can be used to bind proteins at the conductivity of the feed material and to solve specific purification challenges

The multimodal functionality gives a different selectivity compared with traditional ion exchangers including binding of proteins regardless of ionic strength of the loading material . This means that the resin may be used for direct load of clarified feedstocks, without prior dilution to reduce the conductivity of the starting material. To reduce tedious sample preparation, the top filter in HiTrap Capto MMC has been optimized for loading sonicated unclarified cell lysate directly on the column.

The Capto MMC ligand may interact with proteins in many different ways. It contains a carboxyl group, and thus it is a weak cation exchanger. However, in addition to the ionic interactions several other types of interactions are involved, including hydrogen bonding and hydrophobic interaction.