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Electrophoresis Reagents

Gel electrophoresis is a method used in molecular biology to separate DNA and proteins through an electrical current applied based on molecular weight or fragment size. The various reagents, including molecular weight markers, blotting reagents, and stain removal reagents, improve the detection accuracy of electrophoresis results through enhanced visualization. The electrophoresis reagents offered are ideal for use in the fields of molecular biology, immunogenetics, and other molecular biology disciplines.

Kidney tissue region blot, single species

Kidney tissue region blot, single species

Supplier: G-Biosciences

These are ready to screen, normal tissue, kidney tissue region blots from mouse and rat. The kidney tissue specific regions are prepared by carefully dissecting out anatomically and functionally distinct regions of adult mouse and rat kidney.

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picoLUCENT™ PLUS-AP kit

picoLUCENT™ PLUS-AP kit

Supplier: G-Biosciences

The picoLUCENT™ PLUS-HRP kit is based on an ultra sensitive luminol substrate that produces chemiluminescence upon reaction with alkaline phosphatase. The chemiluminescence light emission can be recorded by a short exposure to autoradiography films.

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Immobilon™ Block Noise Canceling Reagents

Immobilon™ Block Noise Canceling Reagents

Supplier: Merck

Immobilon® Block Noise cancelling reagents are designed especially for Western blotting applications. This family of protein-free, ready-to-use buffers has been optimised to reduce background levels when using chemiluminescence, fluorescent, or phosphotyrosine detection.

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Luminata™ Chemiluminescent ELISA HRP Substrates

Luminata™ Chemiluminescent ELISA HRP Substrates

Supplier: Merck

Chemiluminescent HRP detection reagent for use on immunoblots, Western blotting, dot/slot blotting.

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Western blot stripping buffer, Western ReProbe™

Western blot stripping buffer, Western ReProbe™

Supplier: G-Biosciences

Western ReProbe™ is a single component system that is specifically formulated to dissociate and remove antibodies from membrane-bound proteins without destroying the antigenic binding affinity. Western ReProbe™ also enables the ability to reuse Western blots.

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Western ReProbe™ PLUS

Western ReProbe™ PLUS

Supplier: G-Biosciences

Western ReProbe™ PLUS allows for the removal of stubborn, high affinity antibodies from membrane bound proteins without destroying the antigenic binding affinity. The membrane bound protein is retained on the membrane and the matching antibodies are washed away. Once the antigen-antibody bonds are broken, the membrane bound protein is free to accept new antibodies.

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Multiple tissue blot, single species

Multiple tissue blot, single species

Supplier: G-Biosciences

These multiple tissue, single species blots permit researchers to visualise the tissue distribution of their protein in a particular species. These have been successfully used for the analysis of a wide variety of proteins. Each blot has multiple tissue types from either human, mouse or rat species.

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Western blots, pre-made, human single tissue single species

Western blots, pre-made, human single tissue single species

Supplier: G-Biosciences

Ready-to-screen, human, single tissue blots, from both normal and tumour tissues. G-Biosciences pre-made blots are prepared with the appropriate GenLysate™ and 50 μg protein is loaded onto a 4 to 20% denaturing polyacrylamide gel, along with a prestained molecular weight marker. After the proteins are resolved, they are transferred to a PVDF membrane, using our Western transfer buffer.

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Botanical garden blot

Botanical garden blot

Supplier: G-Biosciences

G-Biosciences' Botanical Garden Blot is prepared using total protein extracted from a variety of 5 to 10 day old, whole plant tissues.

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Transfer pads for western blotting, Swift™

Transfer pads for western blotting, Swift™

Supplier: G-Biosciences

SWIFT™ transfer pads are treated with a proprietary electrolyte buffer to enhance Western blot transfer efficiency.

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NPP ELISA alkaline phosphatase substrate

Supplier: Rockland Immunochemicals

NPP ELISA alcaline phosphatase substrate is a chromogenic substrate used in immunosorbent assays.

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Caspase-3 substrates, NucView®

Caspase-3 substrates, NucView®

Supplier: Biotium

NucView® caspase-3 substrates are fluorescent probes that allow for the real-time detection of apoptosis by monitoring caspase-3/7 activity in intact cells using confocal microscopy, flow cytometry or microplate readers.

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Peqlab peqGOLD, Protein Marker II (Unstained)

Peqlab peqGOLD, Protein Marker II (Unstained)

Supplier: VWR Chemicals

Protein Marker II for accurate molecular weight determination of proteins by SDS-PAGE.

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VWR® RiboReady™ Color Micro RNA Ladder

VWR® RiboReady™ Color Micro RNA Ladder

Supplier: VWR Chemicals

RiboReady™ Color Micro RNA ladder allows precise sizing of RNA molecules in denaturing acrylamide gels. The apparent sizes of bands in the RiboReady™ Color Micro RNA ladder are 100, 75, 50, 40, 30 and 20 bases in length.

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Peqlab peqGOLD, DNA Ladder, 1 kb

Peqlab peqGOLD, DNA Ladder, 1 kb

Supplier: VWR Chemicals

DNA ladder for quantitative and qualitative analysis of dsDNA.

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VWR®, DNA ladder, 100 bp

VWR®, DNA ladder, 100 bp

Supplier: VWR Chemicals

Ladder with 13 fragments ranging from 100 to 3000 bp for easy band identification.

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VWR® EZ-Vision®, DNA Ladder, 100 bp

VWR® EZ-Vision®, DNA Ladder, 100 bp

Supplier: VWR Chemicals

EZ-Vision® 100 bp DNA ladder is a pre-stained molecular weight ladder in loading buffer that allows immediate visualisation of DNA ladder post-electrophoresis.

    
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Peqlab peqGOLD, Protein Marker V (Pre-Stained)

Peqlab peqGOLD, Protein Marker V (Pre-Stained)

Supplier: VWR Chemicals

Protein Marker V (pre-stained) for monitoring protein separation, transfer efficiency and size determination.

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VWR® EZ-Vision®, DNA Ladder, 1 kb

VWR® EZ-Vision®, DNA Ladder, 1 kb

Supplier: VWR Chemicals

EZ-Vision® 1 kb DNA ladder is a pre-mixture of DNA marker, loading buffer and a proprietary fluorescent DNA visualisation dye that can be applied directly to the gel. This non toxic, non mutagenic dye eliminates the need for ethidium bromide staining. The 1 kb ladder consists of 10 double-stranded bands with molecular weights between 200 and 10 000 bp. The intensity of individual bands varies to facilitate easy identification.

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VWR®, DNA ladders

VWR®, DNA ladders

Supplier: VWR Chemicals

VWR® DNA ladders are convenient dsDNA ladders supplied in a loading buffer, which are ready to use on agarose and SDS DNA gels.

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VWR®, Protein marker VI (pre-stained), peqGOLD

VWR®, Protein marker VI (pre-stained), peqGOLD

Supplier: VWR Chemicals

peqGOLD pre-stained Protein Marker VI is a mixture of 10 coloured proteins. These proteins are recombinantly produced in E.coli, highly purified and supplied 'ready to use' in loading buffer. The protein concentrations are optimised to yield well-defined bands directly visible in SDS polyacrylamide gels.

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Peqlab peqGOLD, Protein Marker I (Unstained)

Peqlab peqGOLD, Protein Marker I (Unstained)

Supplier: VWR Chemicals

peqGOLD Protein Marker I is a mixture of seven purified proteins which are re-dissolved 'ready to use' in loading buffer. The proteins resolve into sharp, tight bands in the range of 14,4 to 116,0 kDa when analysed by SDS-PAGE and stained with Coomassie Brilliant Blue R-250.

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VWR® Ready Ladder™ DNA Ladder, 50 bp

VWR® Ready Ladder™ DNA Ladder, 50 bp

Supplier: VWR Chemicals

Ladder with 10 fragments ranging from 50 to 500 bp for easy band identification. Ready to use formula pre-mixed with loading dye.

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VWR®, Protein marker III (pre-stained), peqGOLD

VWR®, Protein marker III (pre-stained), peqGOLD

Supplier: VWR Chemicals

Protein Marker III for monitoring protein separation, transfer efficiency and size determination.

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VWR® DNA Molecular Weight Marker, High-Range

VWR® DNA Molecular Weight Marker, High-Range

Supplier: VWR Chemicals

High-Range DNA MW (molecular weight) marker offers 10 fragments ranging from 1503 to 48 502 bp. There is sufficient material for 75 to 150 assays.

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Peqlab peqGOLD, DNA Ladders, 50 bp

Peqlab peqGOLD, DNA Ladders, 50 bp

Supplier: VWR Chemicals

DNA ladder for quantitative and qualitative analysis of dsDNA.

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Peqlab peqGOLD, DNA Ladder, 100 bp

Peqlab peqGOLD, DNA Ladder, 100 bp

Supplier: VWR Chemicals

DNA ladder for quantitative and qualitative analysis of dsDNA.

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Peqlab peqGOLD, Protein Marker IV (Pre-Stained)

Peqlab peqGOLD, Protein Marker IV (Pre-Stained)

Supplier: VWR Chemicals

peqGOLD pre-stained protein marker IV is a mixture of 10 recombinant, highly purified coloured proteins supplied 'ready to use' in loading buffer. The protein concentrations are optimised to yield well-defined bands directly visible in SDS-polyacrylamide gels.

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Peqlab peqGOLD, DNA Ladder, 100 bp Plus

Peqlab peqGOLD, DNA Ladder, 100 bp Plus

Supplier: VWR Chemicals

peqGOLD DNA ladder 100 bp plus is ideal for size determination in the range of 100 to 3000 base pairs. There are no unspecific bands besides the fragments described. 1000 and 500 bp bands have a higher DNA content and serve as a size reference.

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VWR®, DNA ladders

VWR®, DNA ladders

Supplier: VWR Chemicals

VWR® DNA ladders are supplied in a loading buffer that is ready to use on agarose and polyacrylamide DNA gels. The ladders are suitable with TBE, TAE, SB and LB electrophoresis systems.

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