Protein Expression Reagents

Supplier: TriLink BioTechnologies

mCherry mRNA encodes the fluorescent protein, mCherry, which is derived from DsRed, a protein found in Discosoma sp. mCherry is a monomeric fluorophore with a peak absorption at 587 nm and emission at 610 nm. It is stable and resistant to photobleaching.

Supplier: TriLink BioTechnologies

The FLuc mRNA will express a luciferase protein, originally isolated from the firefly, Photinus pyralis. FLuc is commonly used in mammalian cell culture to measure both gene expression and cell viability. It emits bioluminescence in the presence of the substrate, luciferin.

Supplier: TriLink BioTechnologies

Cas9 mRNA expresses a version of the Streptococcus pyogenes SF370 Cas9 protein (CRISPR Associated Protein 9). Cas9 functions as part of the CRISPR (Clustered Regularly Interspaced Short Palindromic Repeats) genome editing system. In the CRISPR system, an RNA guide sequence targets the site of interest and the Cas9 protein is employed to perform double-stranded DNA cleavage.

Supplier: Abcam

His-Tag Protein Expression Check Kit (ab270048) is a quick competitive immunochromatography test that allows users to verify and monitor successful expression of His-tagged recombinant proteins before undertaking purification, as well as identifying fractions of most interest before performing other more labor intensive techniques such as Western blot or ELISA.

Supplier: TriLink BioTechnologies

TriLink’s CleanCap® M6 IVT kit, high yield is a comprehensive solution for mRNA synthesis by in vitro transcription (IVT) with CleanCap® co-transcriptional capping. The kit components are sufficient for 25×100 µl or 125×20 µl reactions under the recommended protocol. When used with the pulse feed protocol, each kit is expected to yield 20 to 25 mg of capped mRNA.

Supplier: TriLink BioTechnologies

The EGFP mRNA expresses an enhanced version of the green fluorescent protein, originally isolated from the jellyfish, Aequorea victoria. EGFP is a commonly used direct detection reporter in mammalian cell culture, yielding bright green fluorescence with an emission peak at 509 nm.

Supplier: TriLink BioTechnologies

The EGFP mRNA expresses an enhanced version of the green fluorescent protein, originally isolated from the jellyfish, Aequorea victoria. EGFP is a commonly used direct detection reporter in mammalian cell culture, yielding bright green fluorescence with an emission peak at 509 nm.

Supplier: TriLink BioTechnologies

Renilla luciferase (Renilla Luc) is a bioluminescent reporter protein derived from Renilla reniformis (sea pansy). In the presence of the cofactor, coelenterazine, Renilla luciferase produces light. Renilla Luc mRNA can be used as an internal control in combination with FLuc mRNA to test different conditions, such as dosage or chemical modifications, and their effect on translation.

Supplier: LENIOBIO

ALiCE® - Linearly scalable eukaryotic cell-free protein expression. Scalable protein expression with ALiCE® works as effectively for assessment of future development as it does in the exploration phase. The same simple protocol applies across all scales. Add the DNA sequence and let ALiCE® make your protein.

Supplier: TriLink BioTechnologies

The FLuc mRNA expresses a luciferase protein, originally isolated from the firefly, Photinus pyralis. FLuc is commonly used in mammalian cell culture to measure both gene expression and cell viability. It emits bioluminescence in the presence of the substrate, luciferin.

Supplier: TriLink BioTechnologies

EPO mRNA encodes the human erythropoietin (EPO) protein, a hormone that controls erythropoiesis, or red blood cell production. EPO also plays a role in wound healing and the brain’s response to neural injury. Kariko et al. showed that transfection of EPO mRNA in vivo resulted in significant increases of both reticulocyte counts and hematocrits.

Supplier: TriLink BioTechnologies

This construct expresses an enhanced version of the green fluorescent protein (EGFP), originally isolated from the jellyfish Aequorea victoria, as self-amplifying RNA (saRNA). EGFP is a commonly used direct detection reporter in mammalian cells, yielding bright green fluorescence with an emission peak at 509 nm.

Supplier: TriLink BioTechnologies

The FLuc mRNA expresses a luciferase protein, originally isolated from the firefly, Photinus pyralis. FLuc is commonly used in mammalian cell culture to measure both gene expression and cell viability. It emits bioluminescence in the presence of the substrate, luciferin.

Supplier: TriLink BioTechnologies

EPO mRNA encodes the human erythropoietin (EPO) protein, a hormone that controls erythropoiesis, or red blood cell production. EPO also plays a role in wound healing and the brain’s response to neural injury. Kariko et al. showed that transfection of EPO mRNA in vivo resulted in significant increases of both reticulocyte counts and hematocrits.

Supplier: LENIOBIO

From ALiCE® for Research to ALiCE® for Scale-Up Protein Expression, we offer cell-free protein expression products with radical results for every production setup.

Supplier: TriLink BioTechnologies

The EGFP mRNA expresses an enhanced version of the green fluorescent protein, originally isolated from the jellyfish, Aequorea victoria. EGFP is a commonly used direct detection reporter in mammalian cell culture, yielding bright green fluorescence with an emission peak at 509 nm.