You searched for: Nucleic Acid Reagents
Nucleic acid reagents form the foundation of genetic analysis and molecular biology, playing a pivotal role from cloning to gene expression studies. Delve into our collection featuring high-fidelity enzymes for end-point PCR, advanced reagents for isothermal amplification, and comprehensive kits for nucleic acid purification. For precision in quantitative assays, explore our selection of qPCR and RT-qPCR kits, primed for unparalleled accuracy. Our portfolio also includes next-generation sequencing reagents, offering cutting-edge solutions for genomic sequencing and personalized medicine advancements.
TriLink® 2',3'-Dideoxycytidine-5'-O-(1-Thiotriphosphate)
Supplier: TriLink BioTechnologies
2',3'-Dideoxycytidine-5'-O-(1-Thiotriphosphate) is a sugar modified nucleoside triphosphate where the 2' and 3' hydroxyl groups are absent, resulting in chain termination. This ddCTP analog also contains a 1-Thiotriphosphate which results in a nuclease resistant phosphorothioate linkage. The inability of polymerases to extend from a dideoxy nucleotide causes chain termination and is useful in antiviral research and in a variety of biotechnology applications.
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miRNA reference kit
Supplier: AGILENT
The Universal Human miRNA Reference RNA is an ideal reference control for miRNA microarray or miRNA-targeted QRTPCR experiments.
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TriLink® 7-Deaza-7-Propargylamino-2'-deoxyadenosine-5'-Triphosphate
Supplier: TriLink BioTechnologies
TriLink® 7-Deaza-7-Propargylamino-2'-deoxyadenosine-5'-Triphosphate
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TriLink® 2'-Deoxyadenosine-5'-Triphosphate
Supplier: TriLink BioTechnologies
This nucleotide consists of an unmodified base, a deoxyribose, and a 5′ triphosphate group for synthesis of DNA molecules.
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TriLink® CleanAmp® dNTP Mix
Supplier: TriLink BioTechnologies
CleanAmp® dNTPs are chemically-modified nucleotides designed to improve specificity, sensitivity, and robustness of your PCR.
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TriLink® CleanAmp® 7-deaza-dGTP Mix
Supplier: TriLink BioTechnologies
CleanAmp® dNTPs are chemically-modified nucleotides designed to improve specificity, sensitivity, and robustness of your PCR.
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Cyanine 3-UTP (enhanced)
Supplier: ENZO LIFE SCIENCES
Cyanine 3-UTP (Enhanced) can replace UTP as a substrate for T7 RNA polymerase in labeling systems that generate labeled probes through in vitro transcription. Probes generated by these methods are suitable for multicolor fluorescence analysis, specifically dual-color expression arrays in conjunction with cyanine 3-UTP .
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QuikChange II
Supplier: AGILENT
These QuikChange II kits provide improved fidelity, while maintaining greater than 80% mutation efficiency for single site mutagenesis.
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Cyanine 5-dUTP
Supplier: ENZO LIFE SCIENCES
Cyanine 5-dUTP can replace TTP in reactions in which it serves as a substrate for E. coli DNA polymerase (holoenzyme and Klenow fragment), T4 and Taq DNA polymerases, reverse transcriptase (from AMV and M-MuLV) and terminal transferase. Fluorescently labeled probes can be prepared with this fluorescent nucleotide by a variety of methods including nick translation, cDNA labeling and 3’-end labeling. Probes generated by these methods are suitable for use for the identification of specific sequences by in situ hybridization procedures on fixed cells and tissues by direct fluorescence detection. Cyanine 5-dUTP can also be used for multicolor fluorescence labeling.
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T4 UvsX DNA Recombinase
Supplier: G-Biosciences
T4 UvsX DNA recombinase mediates DNA strand exchange between homologous chromosomes. The protein forms a right-handed nucleoprotein complex on single ssDNA called the presynaptic filament that can search for homology in duplex DNA and pair the recombining DNA molecules to form a DNA joint molecule. This process is aided by ssDNA binding proteins and recombination mediators. The filament is then elongated by DNA polymerase with the newly synthesised strand displacing the old strand. The newly synthesised duplex DNA can then act as a template for the next cycle to achieve exponential amplification of dsDNA. E.coli cells expressing recombinant T4 UvsX gene.
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SAVIEW® PLUS HRP Reagent
Supplier: ENZO LIFE SCIENCES
High sensitivity, low background streptavidin-based nanopolymer detection reagent for use with HIGHDEF® chromogens in ISH and IHC applications.
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T4 DNA polymerase
Supplier: Thermo Fisher Scientific
T4 DNA Polymerase catalyses the 5'→3' synthesis of DNA from a primed single-stranded DNA template.
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PCR decontamination kit
Supplier: ENZO LIFE SCIENCES
Rapid removal of contaminating DNA in PCR mastermixes, without reduction in PCR sensitivity.
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Site-directed mutagenesis kits, QuikChange Lightning
Supplier: AGILENT
The fastest and latest generation of the market-leading QuikChange kits speeds up the protocol for performing single and multiple site-directed mutagenesis to less than 3 hours.
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Fragment selection kits, Axygen® AxyPrep™ FragmentSelect-I Kit
Supplier: Corning
The AxyPrep™ FragmentSelect Kits utilise a paramagnetic bead technology for quick high throughput optimised DNA size selection suitable for various next generation sequencing platforms. The AxyPrep™ FragmentSelect-I kit is optimised for Illumina next generation sequencing platforms and Life Technologies (SOLiD™) fragment size selection needs simplifying the library construction workflow.
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Restriction enzymes, NotI, 50 U/µl, Fermentas
Supplier: Thermo Fisher Scientific
GGCCGC sites and cuts best at 37 °C in O buffer.
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Restriction enzymes, EcoRI, FastDigest™, Fermentas
Supplier: Thermo Fisher Scientific
Thermo Scientific™ FastDigest™ enzymes are an advanced line of restriction enzymes for rapid DNA digestion.
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Restriction enzymes, NcoI, FastDigest™, fermentas
Supplier: Thermo Fisher Scientific
Thermo Scientific™ FastDigest™ enzymes are an advanced line of restriction enzymes for rapid DNA digestion.
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Restriction enzymes, SfiI, Fermentas
Supplier: Thermo Fisher Scientific
NGGCC sites and cuts best at 50 °C in G buffer.
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Restriction enzymes, NdeI, Fermentas
Supplier: Thermo Fisher Scientific
TATG sites and cuts at 37 °C in O buffer (Isoschizomers: FauNDI).
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Restriction enzymes, NotI, 10 U/µl, Fermentas
Supplier: Thermo Fisher Scientific
GGCCGC sites and cuts best at 37 °C in O buffer.
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Restriction enzymes, PstI, FastDigest™, Fermentas
Supplier: Thermo Fisher Scientific
Thermo Scientific™ FastDigest™ enzymes are an advanced line of restriction enzymes for rapid DNA digestion.
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Restriction enzymes, BsmBI, FastDigest™, fermentas
Supplier: Thermo Fisher Scientific
Thermo Scientific™ FastDigest™ enzymes are an advanced line of restriction enzymes for rapid DNA digestion.
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Restriction enzymes, Restriction enzyme, Eco147I (StuI), Fermentas
Supplier: Thermo Fisher Scientific
CCT sites and cuts best at 37 °C in B buffer (Isoschizomers: AatI, PceI, SseBI, StuI).
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Restriction enzymes, HindIII, 10 U/μl, Fermentas
Supplier: Thermo Fisher Scientific
HindIII restriction endonucleases are a large collection of high quality restriction enzymes, optimized to work in one of the buffers of the Five Buffer System. All of the enzymes exhibit 100% activity in the recommended buffer and reaction conditions.
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BioProbe® 3’-Oligonucleotide labeling system reagent pack
Supplier: ENZO LIFE SCIENCES
The procedure for labeling of DNA probes with a polynucleotide "tail" containing hapten-labeled nucleotides was developed by Enzo. In such terminal labeling reactions, terminal transferase catalyzes the addition of nucleotides to any 3'-OH terminus in a template independent manner. This rapid and convenient nonradioactive labeling procedure is free of any sequence bias that is normally observed in random priming or nick translation reactions. Terminal labeling is an ideal procedure for the labeling of oligonucleotides.
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cDNA synthesis kits, AffinityScript
Supplier: AGILENT
Provides the highest efficiency of conversion of RNA to cDNA, fully optimised for qPCR applications.
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Restriction enzymes, Dpn I restriction enzymes
Supplier: AGILENT
Dpn I is an ideal enzyme for cloning and southern blot analysis.
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Restriction enzymes, BpiI, FastDigest™, fermentas
Supplier: Thermo Fisher Scientific
Thermo Scientific™ FastDigest™ enzymes are an advanced line of restriction enzymes for rapid DNA digestion.
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Restriction enzymes, SalI, FastDigest™, Fermentas
Supplier: Thermo Fisher Scientific
Thermo Scientific™ FastDigest™ enzymes are an advanced line of restriction enzymes for rapid DNA digestion.