You searched for: Protein Purification
Protein purification systems are intended to isolate one or multiple proteins from a complex mixture. Working with cells, tissues, or whole organisms, the machines purify with predefined start points and editable method templates. With a single click, the compact designed equipment offers real-time run control and visualization. Suitable for even cold-room operations, the products enable you to perform purification of affinity-tagged proteins, antibodies, untagged or native proteins, as well as sample clean-up.
Ion exchange chromatography media, Capto™ DEAE
Supplier: Cytiva
Capto™ DEAE is a strong anion exchange bioprocessing medium, designed to meet the demands of modern large-scale manufacturers for fast, efficient, and cost-effective capture and intermediate protein purification when high volume throughput is essential.
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Ion exchange chromatography media, Capto™ S
Supplier: Cytiva
Capto™ S is a strong cation exchange bioprocessing medium, designed to meet the demands of modern large-scale manufacturers for fast, efficient, and cost-effective capture and intermediate protein purification when high volume throughput is essential.
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Ion exchange chromatography media, Capto™ Q
Supplier: Cytiva
Capto™ Q is a strong anion exchange bioprocess medium, designed to meet the demands of modern large-scale manufacturers for fast, efficient, and cost-effective capture and intermediate protein purification when high volume throughput is essential.
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Capto™ MMC ImpRes multimodal chromatography media, Cytiva
Supplier: Cytiva
Capto MMC ImpRes can be used for initial or final polishing steps in MAb purification processes, as well as for polishing of antibody fragments such as domain antibodies (DAbs). The wide window of operation for conductivity and/or pH simplifies the process as conditioning after the protein A step can be omitted.
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Capto™ adhere ImpRes multimodal chromatography media, Cytiva
Supplier: Cytiva
Capto™ adhere ImpRes is a strong anion exchange multimodal BioProcess chromatography medium (resin), designed to meet the demands of modern large-scale manufacturers, for high-resolution polishing of monoclonal antibodies (MAbs) and other biomolecules. Capto™ adhere ImpRes is designed to allow effective MAb polishing in the second or third step of a purification scheme downstream of the protein A capture step. Polishing can be performed in either bind and elute (binding) or flowthrough (non binding) modes.
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Immobilised metal ion affinity chromatography (IMAC) media, Ni Sepharose™ excel
Supplier: Cytiva
Ni Sepharose™ excel is an IMAC medium pre-charged with nickel ions very strongly bound to a chelating ligand. Samples that usually cause stripping of metal ions can, therefore, be loaded to the medium. Ni Sepharose™ excel is designed primarily for capture and purification of histidine-tagged proteins secreted into cell culture supernatants from eukaryotic cells such as insect cells or CHO cells.
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Accessories for chromatography systems
Supplier: Cytiva
UniTag sheet with 108 labels
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Affinity chromatography media, HiTrap™ Capto™ Lentil Lectin
Supplier: Cytiva
Capto™ Lentil Lectin is an affinity chromatography resin for purification of glycoproteins and other molecules containing carbohydrates such as α-D-mannose and α-D-glucose or sterically related residues.
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Protease affinity chromatography media, PreScission™, Cytiva
Supplier: Cytiva
Enables the low-temperature cleavage of fusion proteins containing the PreScission Protease recognition sequence.
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Affinity chromatography media, IMAC Sepharose™ 6 Fast Flow
Supplier: Cytiva
IMAC Sepharose 6 Fast Flow is composed of cross-linked 6% agarose beads modified with a novel chelating ligand immobilized to the base matrix.
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Gel filtration media, Sephadex™ G-15
Supplier: Cytiva
Sephadex™ G-15 is a well established gel filtration medium for desalting and buffer exchange of peptides and small biomolecules >1500 molecular weight.
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Affinity chromatography media, IgG Sepharose™ 6 Fast Flow
Supplier: Cytiva
lgG Sepharose™ 6 Fast Flow, from Cytiva, is based on the rigid Sepharose 6 Fast Flow matrix, with human IgG covalently coupled to it.
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Ion exchange chromatography media, SP Sepharose™ High Performance
Supplier: Cytiva
SP Sepharose™ High Performance is composed of crosslinked agarose beads with a mean diameter of 34 µm, modified with sulphopropyl (SP) strong cation exchange groups.
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Ion exchange chromatography media, IEX Sepharose™ Fast Flow
Supplier: Cytiva
Q Sepharose Fast Flow is part of the Sepharose Fast Flow ion exchange platform, which has been the industrial standard for ion exchange chromatography during recent decades.
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Sepharose™ 4B Gel Filtration Base Matrix
Supplier: Cytiva
Sepharose™ 4B is an agarose gel filtration base matrix and is frequently used for coupling affinity ligands to the matrix. The matrix is not pre-activated and the user performs all steps in coupling.
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Gel filtration media, Sephadex™ G-50
Supplier: Cytiva
Sephadex™ G-50 Medium is well established gel filtration medium for desalting and buffer exchange of biomolecules >30000 molecular weight. The larger bead size of G-50 Medium is suited for large scale separations.
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Sepharose™ 4 FF Octyl Fast Flow Hydrophobic Interaction Chromatography Media
Supplier: Cytiva
Octyl Sepharose™ 4 Fast Flow resin is based on cross-linked 4% agarose. The aliphatic ligand is immobilised to the base matrix with an ether linkage. The ligand contains no charged groups, making true hydrophobic interaction chromatography possible, without interfering ionic effects.
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Plasmid purification media, PlasmidSelect Xtra
Supplier: Cytiva
PlasmidSelect Xtra is a thiophilic aromatic adsorption chromatography medium with a selectivity that allows generic purification of supercoiled covalently closed circular forms of plasmid DNA to be separated from open circular forms.
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Affinity chromatography media, MabSelect™
Supplier: Cytiva
MabSelect™ is a protein A affinity resin designed for capturing monoclonal antibodies (mAbs) from large sample volumes.
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Affinity chromatography media, Benzamidine Sepharose™ 4 Fast Flow (High Sub)
Supplier: Cytiva
Benzamidine Sepharose™ 4 Fast Flow (High Sub) is a tool for removal and/or purification of trypsin and trypsin-like serine proteases, as well as zymogens, including urokinase and prekallikrein.
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Con A Sepharose™ 4B Affinity Chromatography Media, Cytiva
Supplier: Cytiva
Con A Sepharose is Concanavalin A coupled to Sepharose 4B by the cyanogen bromide method.
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Gel filtration media, Superdex™ 30 PG
Supplier: Cytiva
Superdex prep grade is a preparative gel filtration medium with a composite matrix of dextran and agarose
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CM Sephadex™ C-25 Ion Exchange Chromatography Media
Supplier: Cytiva
Sephadex ion exchangers are produced by introducing functional groups onto the cross-linked dextran matrix.
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Sepharose™ CL-6B Gel Filtration Base Matrix
Supplier: Cytiva
Sepharose™ CL-6B is a cross-linked agarose gel filtration base matrix and is frequently used for coupling affinity ligands to the matrix.
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Hydrophobic interaction chromatography media, Butyl Sepharose™ 4 Fast Flow
Supplier: Cytiva
Butyl Sepharose 4 Fast Flow is part of the Sepharose Fast Flow HIC platform, which has been an industrial standard for HIC chromatography during recent decades.
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Affinity chromatography media, MabSelect SuRe™
Supplier: Cytiva
MabSelect SuRe™ is composed of a rigid, high-flow agarose matrix and alkali-tolerant rProtein A ligand. The ligand has been engineered to provide greater stability than conventional protein A-based media in the alkaline conditions used in CIP protocols. This enhanced alkali stability improves process economy and product quality.
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Ion exchange chromatography media, DEAE Sephacel™
Supplier: Cytiva
DEAE Sephacel™ is a weak anion exchanger based on beaded cellulose. The ion exchange group is diethylaminoethyl, which remains charged and maintains consistently high capacity over the entire working range. This media is macroporous and has an exclusion limit of ~10000 kDa for globular proteins. Supplied pre-swollen in 20% ethanol.
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Affinity coupling media, Epoxy-Activated Sepharose™ 6B
Supplier: Cytiva
Epoxy-activated Sepharose™ 6B is a pre-activated medium for immobilisation of various ligands including sugars through coupling of hydroxy, amino or thiol groups on the ligand to Sepharose™ 6B via a 12-atom hydrophilic spacer arm. Medium is formed by reacting Sepharose™ 6B with 1,4-bis (2,3- epoxy-propoxy-) butane. It can be used to couple sugars and other carbohydrates via stable ether linkages to hydroxyl groups.
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Protein G, MP Biomedicals
Supplier: MP Biomedicals
Protein G is a monomeric protein lacking cysteine residues. Recombinant Protein G is a highly stable surface receptor that is capable of binding the Fc portion of immunoglobulins, especially IgGs from a large number of species.
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Gel filtration media, Sephadex™ G-25
Supplier: Cytiva
Sephadex™ G-25 is well established gel filtration medium for desalting and buffer exchange in industrial applications.