You searched for: Protein Purification

Supplier: Merck Millipore (Novagen)

S-protein agarose specifically retains STag™ fusion proteins. The capacity of S-protein agarose varies and is based on the size and folding characteristics of a given target protein.

Supplier: G-Biosciences

Immobilised β-cyclodextrin is designed to allow refolding of recombinant proteins that were aggregated or formed inclusion bodies when expressed in the genetically engineered cells. The refolding of misfolded proteins is adapted from GroE chaperone refolding mechanism.

Supplier: Merck Millipore (Novagen)

GST Bind™ Resin utilizes an 14-atom spacer arm to covalently attach reduced glutathione via a sulfide linkage. The high degree of substitution of glutathione ensures a high binding capacity with yields of glutathione S-transferase (GST) fusion proteins of 5 to 8 mg/ml settled resin. The resin can be re-used up to 6 times without loss of capacity.

Supplier: Merck Millipore (Novagen)

D-Desthiobiotin provides a gentle elution of StrepTag® II proteins from the biotin-binding site of StrepTactin® resins. D-Desthiobiotin is offered as a lyophilised powder for preparation of elution buffer.

Supplier: Thermo Fisher Scientific

Carboxy-PEG₁₂-thiol, is a carboxyl- and sulphhydryl-terminated compound that contains a 12-unit polyethylene glycol (PEG) spacer and is used to modify surfaces such as quantum dots, monolayers and magnetic particles.

Supplier: Merck Millipore (Calbiochem‎)

A ready-to-use stable, neutral form of the disulfide-reducing agent TCEP, hydrochloride.

Supplier: Thermo Fisher Scientific

MALDI Matrices are highly purified, recrystallised reagents packaged in convenient single-use tubes sufficient for analysing up to 96 samples.

Supplier: Thermo Fisher Scientific

Provides a complete set of reagents and a simple, fast and reproducible protocol to perform chromatin immunoprecipitation (ChIP) assays.

Supplier: G-Biosciences

This Sulphhydryl Immobilisation Kit is designed for the coupling of sulphhydryl containing peptides to a solid agarose support. The sulphhydryl coupling resin columns utilises iodoacetyl groups that specifically react with free sulphhydryls to form covalent, permanent thioether bonds.

Supplier: Thermo Fisher Scientific

Pierce™ Co-Immunoprecipitation (Co-IP) Kit provides covalent antibody immobilisation and all the components necessary to perform a properly controlled co-IP experiment without antibody interference in the final product.

Supplier: Thermo Fisher Scientific

Methyl-PEG₄-thiol is a methyl- and sulfhydryl-terminated compound that contains a 4-unit polyethylene glycol (PEG) spacer and is used to modify surfaces such as quantum dots, monolayers and magnetic particles.

Supplier: Merck Millipore (Novagen)

The Enterokinase Cleavage Capture Kit is designed for highly specific cleavage of fusion proteins followed by rapid, affinity-based capture and removal of enterokinase. Following cleavage of the target protein, rEK is removed with >99% efficiency from the reaction by affinity capture on EKapture™ Agarose. Following capture of rEK, the EKapture Agarose is removed by spin filtration, because the same buffer conditions are used for both cleavage and capture no buffer changes are necessary.

Supplier: Thermo Fisher Scientific

The methyl-PEG_n-amine (MA[PEG]_n) PEGylation reagents are methyl ether-terminated PEG amines that are used for modifying proteins or surfaces such as beads, nanoparticles and self-assembled monolayers. These PEGylation reagents are homogenous compounds of defined molecular weight and spacer length, providing precision in optimising modification applications.

Supplier: Cytiva

Capto™ Phenyl (high sub) is used in the capture and intermediate stages of protein purification.

Supplier: Cytiva

Capto™ Blue is an affinity medium for the capture of albumin, purification of enzymes, and recombinant proteins at laboratory and process scales.

Supplier: Cytiva

Dextrin Sepharose™ High Performance is an affinity media for purification of recombinant proteins tagged with Maltose Binding Protein (MBP).

Supplier: Cytiva

Cobalt-based chromatography medium designed for purification of histidine-tagged recombinant proteins by immobilised metal affinity chromatography (IMAC).

Supplier: Cytiva

Capto™ SP ImpRes is a strong cation exchange bioprocessing medium, designed to meet the demands of modern large-scale manufacturers for fast, efficient, and cost-effective intermediate and polishing protein purification.

Supplier: Cytiva

IMAC Sepharose High Performance media is easily charged by the user with the metalion of choice for Immobilized Metal ion Affinity Chromatography (IMAC) and subsequent purification of polyhistidine tagged proteins.

Supplier: Cytiva

Capto™ Q ImpRes is a strong anion exchange bioprocess medium, designed to meet the demands of modern large-scale manufacturers for fast, efficient, and cost-effective intermediate and polishing protein purification.

Supplier: Cytiva

Glutathione Sepharose 4 Fast Flow is used for the purification of GST-tagged proteins from bacterial lysates and other S-tranferases or glutathione-dependent proteins.

Supplier: Cytiva

Glutathione Sepharose High Performance is used for the purification of GST-tagged proteins from bacterial lysates and other S-transferases or glutathione-dependent proteins.

Supplier: Cytiva

Ni Sepharose 6 Fast Flow is a BioProcess medium that combines the advantages of Ni₂₊ for purification of histidine tagged proteins, with the well industrially established properties of the Sepharose Fast Flow platform.

Supplier: Cytiva

Glutathione Sepharose™ 4B is designed for high capacity single-step purification of Glutathione S-Transferase (GST) tagged fusion proteins.

Supplier: Cytiva

Capto™ DEAE is a strong anion exchange bioprocessing medium, designed to meet the demands of modern large-scale manufacturers for fast, efficient, and cost-effective capture and intermediate protein purification when high volume throughput is essential.

Supplier: Cytiva

Sephacryl™ High Resolution (HR) gel filtration media allow fast and reproducible purification of proteins, polysaccharides, and other macromolecules by gel filtration at laboratory and industrial scale.

Supplier: Cytiva

A chromatography resin for purification of recombinant proteins tagged with Strep-tag II and Twin-Strep-tag. These proteins bind very specifically to the immobilised Strep-Tactin XT ligand giving highly pure target protein.

Supplier: Cytiva

Capto MMC ImpRes can be used for initial or final polishing steps in MAb purification processes, as well as for polishing of antibody fragments such as domain antibodies (DAbs). The wide window of operation for conductivity and/or pH simplifies the process as conditioning after the protein A step can be omitted.

Supplier: Cytiva

Ni Sepharose™ High Performance affinity media for high resolution, high capacity purification of histidine-tagged proteins by immobilised metal ion affinity chromatography (IMAC).

Supplier: Cytiva

Capto™ Butyl is used in the capture and intermediate stages of protein purification. The combination of high capacity, high flow rate and low back pressure reduces process cycle times.