You searched for: Protein Assays

Supplier: Thermo Fisher Scientific

The Pierce™ Coomassie Plus Protein assay is a quick and ready-to-use, reducing agent-compatible, improved Bradford assay to quickly measure (A595 nm) total protein concentration compared to a protein standard.

Supplier: Thermo Fisher Scientific

Pierce BCA™ (bicinchoninic acid) reagents provide accurate determination of protein concentration with most sample types encountered in protein research. The Pierce BCA™ Protein Assay can be used to assess yields in whole cell lysates and affinity-column fractions, as well as to monitor protein contamination in industrial applications. Compared to most dye-binding methods, the BCA™ Assay is affected much less by protein compositional differences, providing greater protein-to-protein uniformity.

Supplier: Thermo Fisher Scientific

Pierce™ Phosphoprotein Phosphate Estimation Assay Kit enables classification and identification of proteins as phosphorylated (serine and threonine), as well as semi-quantitative assessment of the phosphorylation level.

Supplier: Thermo Fisher Scientific

Pierce™ Glycoprotein Carbohydrate Estimation Kit enables the amount of protein glycosylation to be measured as a percentage of total purified protein mass.

Supplier: Thermo Fisher Scientific

The Pierce™ Coomassie (Bradford) Protein Assay Kit is a ready-to-use, stable formulation of the traditional Bradford assay reagent to measure (A595 nm) total protein concentration compared to a protein standard.

Supplier: Thermo Fisher Scientific

Pierce™ 660 nm Protein Assay is a ready to use, detergent- and reducing agent-compatible assay reagent to quickly measure (A660 nm) total protein concentration compared to a protein standard.

Supplier: Thermo Fisher Scientific

The Pierce™ his protein interaction pull-down kit contains the necessary components to capture and purify proteins that interact with his-tagged fusion proteins.

Supplier: Thermo Fisher Scientific

This BCA Protein Assay Kit - Reducing Agent Compatible enables the measurement of protein concentration in samples that contain thiol-reductants dithiothreitol (DTT) and 2-mercaptoethanol (BME).

Supplier: Thermo Fisher Scientific

Biotinylated horse radish peroxidase is used as control or signal amplification in IHC to amplify the signal of biotinylated primary antibodies using the avidin-biotin complex staining method.

Supplier: Thermo Fisher Scientific

The Compat-Able™ Reagents remove salts, detergents, reducing agents and other substances from protein samples to eliminate interference with protein assays. The protocol selectively precipitates all of the protein, thereby allowing the non-protein components to be easily decanted and removed. The purified, precipitated protein is redissolved in water, making it ready for accurate determination of the protein concentration by standard protein assay methods. The procedure is accomplished in less than 10 min with just a few simple steps. The Compat-Able™ Preparation Set is available conveniently bundled with the Pierce BCA™ Protein Assay Kit.

Supplier: OZ BIOSCIENCES

Bradford Protein Assay Kit (B-Pak) is a straightforward and rapid kit for determining the concentration of proteins in solution. This ready to use kit is provided with 1X reagent and 2 sets of BSA standards which means no dilution, filtration or calculation required. B-Pak is provided with adapted protocols for all experimental conditions – micro and macroassays.

Supplier: Thermo Fisher Scientific

Pierce™ Protease Assay Kits are an ideal choice for performing routine assays necessary during the isolation of proteases, or for identifying the presence of contaminating proteases in protein samples. These protease assays are also ideal for studying pH or temperature vs. activity profiles of purified proteases.

Supplier: Thermo Fisher Scientific

Marfey's Reagent (FDAA or 1-fluoro-2-4-dinitrophenyl-5-L-alanine amide) reacts with primary amines to enable the quick and easy separation and quantitation of optical isomers of amino acids by reverse-phase chromatography.

Supplier: AAT BIOQUEST

Optimised for CytoCite™ and Qubit™ fluorometers. The Portelite™ fluorimetric protein quantitation kit is significantly more sensitive than existing colorimetric protein measurements, e.g., Bradford and Bicinchoninic acid (BCA) assays.

Supplier: 3M Food Safety

The 3M™ Allergen Protein Rapid Kits are intended for screening for the presence of specific proteins in clean-in-place (CIP) final rinse water, environmental swab samples, food ingredients and processed food products.

Supplier: Thermo Fisher Scientific

Pierce™ biotinylated protein interaction pull-down kit contains the necessary components to capture and purify interactors of a biotin-labeled protein or ligand.

Supplier: Thermo Fisher Scientific

Pierce™ Biotin Quantitation Kit includes aliquots of HABA reagent and biotinylated protein standard for convenient colorimetric determination of biotinylation levels in labeled antibodies and other proteins.

Supplier: AAT BIOQUEST

Designed for consistent and dependable results. Amplite® Fluorimetric Biotin Quantitation Kit uses Biotinylite™ Green, a fluorogenic biotin sensor.

Supplier: Thermo Fisher Scientific

The Pierce™ GST Interaction Pull-Down Kit contains the necessary components to capture and purify proteins that interact with GST-tagged fusion proteins.

Supplier: Thermo Fisher Scientific

Thermo Scientific™ Pierce™ Quantitative Fluorescent Peptide Assay is a sensitive, mix-and-read fluorescent microplate assay for the quantitative measurement of peptides and peptide mixtures.

Supplier: Thermo Fisher Scientific

The Pierce™ Firefly Luciferase Flash Assay Kit provides reagents to measure red firefly luciferase activity in whole cell producing a very strong flash signal which decays rapidly.

Supplier: AAT BIOQUEST

REASH acetate is a cell-permeable version of REASH, which is a resorufin derivative, modified to contain two arsenic atoms at a set distance from each other.

Supplier: AAT BIOQUEST

FLASH diacetate is a cell-permeable version of FlASH, which is a fluorescein derivative, modified to contain two arsenic atoms at a set distance from each other.

Supplier: Thermo Fisher Scientific

The Pierce™ Firefly Luciferase Glow Assay Kit provides a bright and stable bioluminescence signal (half-life greater than 2 hours) in the presence of firefly luciferase reporters for protein expression assays.

Supplier: AAT BIOQUEST

The acridinium NHS ester can be used to label proteins and nucleic acids.

Supplier: AAT BIOQUEST

TCEP is a common reducing reagent widely used to break disulfide bonds within and between proteins.

Supplier: Sartorius

For label-free quantitation and kinetic analysis of his-tagged proteins.

Supplier: AAT BIOQUEST

Protein quantification is an essential task in protein purification, electrophoresis, cell biology, molecular biology and other research applications.

Supplier: AAT BIOQUEST

Optimised to use with CytoCite™ and Qubit™ fluorometers. Portelite™ rapid fluorimetric biotin quantitation kit is specifically optimised to quantify biotin and biotin conjugates with a portal fluorometer such as CytoCite™ and Qubit™ fluorometer.

Supplier: Thermo Fisher Scientific

This antibody labeling system uses nickel chelated agarose to temporarily immobilise antibody molecules via their histidine-rich Fc regions. Once held in place on the gel, the antibody can be biotinylated at either sulfhydryl groups (after mild reduction or disulfide bonds) or primary amines. Excess labeling reagent and by-products are then washed away before recovering the labeled and purified antibody from the gel using a mild imidazole solution. No gel filtration or dialysis are needed.