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You searched for: Cellular Assays

Cellular Assays

Cellular assays have always been a powerful tool in the research lab. They are incredibly versatile, and can be designed to measure virtually any cellular or biochemical function. Sensitive detection of fluorescent probes aid in evaluating cell signaling and death pathways, which is useful for the rapid quantification of cell viability. Cellular assays can be used to measure the effects of different treatments on cell viability and to evaluate toxic effects mediated through a variety of mechanisms.

Steady membrane staining kits, CellBrite™

Steady membrane staining kits, CellBrite™

Supplier: Biotium

Cell surface staining kits with fluorescent membrane dye plus enhancer for imaging live cell surface for several hours to days.

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Apoptosis/Necrosis Detection Kits, for microscopy and flow cytometry, GFP-Certified™

Apoptosis/Necrosis Detection Kits, for microscopy and flow cytometry, GFP-Certified™

Supplier: ENZO LIFE SCIENCES

GFP-Certified™ Apoptosis/Necrosis Detection Kit is a multiplex assay that distinguishes between healthy, early apoptotic, late apoptotic and necrotic cells, compatible with GFP and other green fluorescent probes.

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NUCLEAR-ID® green cell cycle kit for flow cytometry

Supplier: ENZO LIFE SCIENCES

For cell cycle results independent of incubation time, temperature, dye and cell concentrations.

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CF® Dye Dextran 150000 MW, anionic and fixable

CF® Dye Dextran 150000 MW, anionic and fixable

Supplier: Biotium

CF® dye labeled dextrans could be used as a fluorescent fluid-phase markers to study cell permeability, endocytosis, or mechanisms of biomolecular delivery. The dextran is 150,000 MW, and contains a formaldehyde-fixable free-amine group.

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Ubiquitin conjugating kit (HeLa lysate-based)

Supplier: ENZO LIFE SCIENCES

1. Ubiquitinylation of specific endogenous HeLa lysate proteins, followed by their immediate detection/analysis using antibodies to the protein/s of interest, indicating a particular protein is a substrate for the ubiquitin-proteasome pathway. 2. Ubiquitinylation of proteins of interest contained in exogenously added expression culture/cell extracts or tissue lysates/extracts, followed by their immediate detection/analysis or isolation/purification for use in subsequent experiments. 3. Modification of proteins using ubiquitin derivatives or mutants for improved detection/analysis or investigation of alternative (non-proteasomal) ubiquitin related pathways in subsequent experiments. For example, biotinylated-ubiquitin (Prod. No. BML-UW8705), for sensitive detection

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CREB (Phospho-Ser133) transcription factor assay kit

Supplier: Cayman Chemical

A 96-well assay for measurement of CREB.

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FLUOR DE LYS®-Green HDAC2 fluorometric drug discovery assay kit

Supplier: ENZO LIFE SCIENCES

The HDAC2 Fluorescent Activity Assay/Drug Discovery Kit is a complete assay system designed to measure the lysyl deacetylase activity of the recombinant human HDAC2 included in the kit. The kit is ideal for chemical library screening for candidate inhibitors or kinetic assay of the enzyme under varying conditions.

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LEADING LIGHT® Wnt cell line medium pack

Supplier: ENZO LIFE SCIENCES

For use with the LEADING LIGHT® Wnt Reporter Cell Line.

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NUCLEAR-ID® green chromatin condensation detection kit

Supplier: ENZO LIFE SCIENCES

This kit provides a rapid assay for nuclear condensation, a prominent hallmark of apoptosis.A highly permeable green-emitting dye for enhanced detection of apoptosis-induced chromatin condensation.

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Membrane potential assay kit, FLIPR®

Membrane potential assay kit, FLIPR®

Supplier: Molecular Devices

The FLIPR® Membrane Potential Assay Kit provide a fast, simple and reliable, fluorescence-based assay for detecting changes in voltage across the cell membrane and its homogenous fluorescence-based formulations for observation of real-time membrane potential changes associated with ion channel activation and ion transporter proteins.

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NETosis Assay Kit

NETosis Assay Kit

Supplier: Cayman Chemical

For the measurement of NET-derived neutrophil elastase.

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Mito-ID® Membrane potential cytotoxicity kit for microplates

Supplier: ENZO LIFE SCIENCES

A Real-time Mitochondrial membrane Potential Assay with Superior Sensitivity. Suitable for high-throughput applications.

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Prostaglandin Screening Library II

Prostaglandin Screening Library II

Supplier: Cayman Chemical

For D and E series prostaglandin analog screening.

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JC-1 Mitochondrial Membrane Potential Detection Kit, Biotium

JC-1 Mitochondrial Membrane Potential Detection Kit, Biotium

Supplier: Biotium

JC-1 dye is used for fluorescence-based measurement of mitochondrial membrane potential changes in cells, a key measure of cell viability.

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ROS-ID™ ROS/RNS Detection kit

Supplier: ENZO LIFE SCIENCES

This kit is designed to directly monitor real time reactive oxygen and/or nitrogen species (ROS/RNS) production in live cells using fluorescence microscopy.

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Bacterial Ssaining, viability/cytotoxicity assay kit for bacteria live and dead cells

Supplier: Biotium

The Bacteria Viability/Cytotoxicity Assay Kit provides two-colour fluorescence staining of both live bacteria (green) and dead bacteria (red) using two probes, DMAO and EthD-III.

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Red detection kit (GFP-CERTIFIED®), MITO-ID®

Supplier: ENZO LIFE SCIENCES

MITO-ID® Red Detection Kit (GFP-CERTIFIED®) is a photostable, non-toxic and selective mitochondrial dye that stains live, detergent permeabilised and aldehyde fixed cells. This kit is specifically designed for use with GFP expressing cell lines, as well as cells expressing blue, cyan or yellow fluorescent proteins (BFP's, CFP's, YFP's).

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Red detection kit (GFP-CERTIFIED®), LYSO-ID®

Red detection kit (GFP-CERTIFIED®), LYSO-ID®

Supplier: ENZO LIFE SCIENCES

LYSO-ID® Red Detection Kit (GFP-CERTIFIED®) is an acidic organelle-selective dye for live cell staining of lysosomes. It is an ideal choice for fluorescence co-localisation imaging with GFP-tagged proteins and providing spectrally pure red signal.

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LYSO-ID® red cytotoxicity kit (GFP-CERTIFIED®) for microplates

Supplier: ENZO LIFE SCIENCES

A Rapid, Quantitative and HTS‐compatible Live Cell Cytotoxicity Assay

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Apoptosis assay kit (Dual), NucView™ 488

Apoptosis assay kit (Dual), NucView™ 488

Supplier: Biotium

NucView® 488 and CF™dye (594, 640R and Texas RED) conjugated annexin V provides two apoptosis events, caspase-3 activity and phosphatidylserine (PS) translocation.

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ROS-ID™ total ROS/superoxide detection kit

ROS-ID™ total ROS/superoxide detection kit

Supplier: ENZO LIFE SCIENCES

The kit is designed to directly monitor real time reactive oxygen species (ROS) production and Specifically Superoxide in live cells using fluorescence microscopy or flow cytometry.

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Fluorescent calcium indicator, GFP-CERTIFIED® FLUOFORTE®

Supplier: ENZO LIFE SCIENCES

GFP-CERTIFIED® FLUOFORTE® is a fluorescent calcium indicator relative to other red fluorescent dyes.

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Nuclear-ID™ red cell cycle kit, GFP-CERTIFIED®, for flow cytometry

Supplier: ENZO LIFE SCIENCES

GFP-Certified™ Nuclear-ID™ Red Cell Cycle Analysis Kit provides a convenient approach for studying the induction and inhibition of cell cycle progression by flow cytometry.

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QBT™ Fatty acid uptake assay kit

QBT™ Fatty acid uptake assay kit

Supplier: Molecular Devices

QBT™ Fatty Acid Uptake Assay Kit is a homogeneous assay amenable to high-throughput screening. The kits deliver pre-optimized, fluorescence-based formulations to expedite assay development and screening of fatty acid transporters.

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FLUOR DE LYS® HDAC8 fluorometric drug discovery kit

Supplier: ENZO LIFE SCIENCES

A Fluor-de-Lys® fluorescent assay system. The HDAC8 Fluorescent Activity Assay/Drug Discovery Kit is a complete assay system designed to measure the lysyl deacetylase activity of the recombinant human HDAC8 included in the kit. The kit is ideal for chemical library screening for candidate inhibitors or activators or kinetic assay of the enzyme under varying conditions. The Fluor de Lys® HDAC8 assay is based on the Fluor de Lys® Substrate and Fluor de Lys® Developer combination. The assay procedure has two steps. First, the Fluor de Lys® Substrate, which comprises an acetylated lysine side chain, is incubated with HDAC6. Deacetylation of the substrate sensitizes the substrate so that, in the second step, treatment with the Fluor de Lys®Developer produces a fluorophore.

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Reporter assay kits, SpectraMax® Glo Steady-Luc™

Reporter assay kits, SpectraMax® Glo Steady-Luc™

Supplier: Molecular Devices

The SpectraMax® Glo Steady-Luc™ Reporter Assay Kit provides a highly sensitive assay for the quantitation of firefly luciferase expression in mammalian cells. The assay kit is optimised for Molecular Devices SpectraMax® microplate readers with a preconfigured protocol provided in SoftMax® Pro Software for simplified data acquisition and analysis.

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ViaFluor® 405 live cell microtubule stain 1000X in DMSO

Supplier: Biotium

Stain microtubules in live cells with blue fluorescence.

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Lyso-ID® red detection kit (GFP-CERTIFIED®)

Lyso-ID® red detection kit (GFP-CERTIFIED®)

Supplier: ENZO LIFE SCIENCES

For use with GFP-expressing cell lines, as well as cells expressing blue, cyan or yellow fluorescent proteins.

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Prostaglandin Screening Library I

Prostaglandin Screening Library I

Supplier: Cayman Chemical

For screening of PGF analogs.

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TOTAL-NUCLEAR-ID® green/red nucleolar/nuclear detection kit

Supplier: ENZO LIFE SCIENCES

The kit contains two proprietary dyes suitable for visualizing nucleoli and nuclei simultaneously in living cells.

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