You searched for: Cellular Assays

Supplier: Cayman Chemical

Neutrophil/Monocyte Respiratory Burst Assay Kit provides PMA, dihydrorhodamine 123, and additional reagents necessary for inducing and quantifying a respiratory burst response in neutrophils and monocytes by flow cytometry. The assay can be performed on whole blood or on cells in various types of cell culture media. Because the assay reagents are not species-specific, this assay can be used in any species or cell type capable of producing a NADPH oxidase-dependent respiratory burst response.

Supplier: Molecular Devices

SpectraMax® DuoLuc™ reporter assay kit offers sensitive detection of dual luciferase reporter gene assays, which are important tools for monitoring gene expression and other cellular events.

Supplier: Cayman Chemical

96 well assay for measurement of SREBP-2.

Supplier: Cayman Chemical

Uric Acid Assay Kit is used to measure uric acid in serum, plasma and urine.

Supplier: Biotium

Stain microtubules in live cells with far-red fluorescence.

Supplier: Biotium

This NucView™488 and RedDot™2 Apoptosis and Necrosis Assay Kit contains NucView™488 caspase-3 substrate for detection of caspase-3/7 activity. The far-red dead-cell stain RedDot™2 is included for staining necrotic and late apoptotic cells that have compromised plasma membrane integrity. This kit provides a convenient tool for profiling apoptotic and necrotic cell populations by fluorescence microscopy or flow cytometry.

Supplier: ENZO LIFE SCIENCES

For the detection of Cytochrome c translocation from mitochondria into cytosol during apoptosis.

Supplier: ENZO LIFE SCIENCES

For use with the LEADING LIGHT® Wnt Reporter Cell Line.

Supplier: Cayman Chemical

For the determination of total calcium concentration in a variety of biological samples, as well as tissue homogenates and cell lysates.

Supplier: ENZO LIFE SCIENCES

Based on the dye cresyl violet and the cathepsin B ArgArg peptide recognition sequence, this kit allows the detection of cathepsin B activity in living cells by fluorescence microscopy.

Supplier: AGILENT

These are used in all Big Blue rodents and cell lines.

Supplier: Cayman Chemical

Measure superoxide and hydrogen peroxide levels in living cells.

Supplier: Biotium

The Bacteria Viability/Cytotoxicity Assay Kit provides two-colour fluorescence staining of both live bacteria (green) and dead bacteria (red) using two probes, DMAO and EthD-III.

Supplier: Biotium

TUNEL (terminal deoxynucleotidyl transferase (TdT) mediated dUTP nick-end labeling) is highly selective for the detection of apoptotic cells, but not necrotic cells or cells with DNA strand breaks resulting from irradiation or drug treatment. In this assay, TdT enzyme catalyses the addition of labeled dUTP to the 3’ ends of cleaved DNA fragments. Fluorescent dye-conjugated dUTP can be used for direct detection of fragmented DNA by fluorescence microscopy or flow cytometry.

Supplier: Biotium

This kit is designed to measure firefly luciferase activity in transfected cell reporter assays.

Supplier: ENZO LIFE SCIENCES

Specifically designed for visualizing microscopically nucleoli in living cells.

Supplier: Biotium

CF® dye labeled dextrans could be used as a fluorescent fluid-phase markers to study cell permeability, endocytosis, or mechanisms of biomolecular delivery. The dextran is 10000 MW, and contains a formaldehyde-fixable free-amine group.

Supplier: Biotium

CF® dye labeled dextrans could be used as a fluorescent fluid-phase markers to study cell permeability, endocytosis, or mechanisms of biomolecular delivery. The dextran is 70,000 MW, and contains a formaldehyde-fixable free-amine group.

Supplier: Biotium

CF® dye labeled dextrans could be used as a fluorescent fluid-phase markers to study cell permeability, endocytosis, or mechanisms of biomolecular delivery. The dextran is 250,000 MW, and contains a formaldehyde-fixable free-amine group.

Supplier: ENZO LIFE SCIENCES

For the effective isolation of a highly enriched mitochondrial fraction from the cytosolic fraction of various mammalian cells including both apoptotic an non-apoptotic cells. The enriched mitochondrial and cytosolic fractions can be used to study apoptosis and signal trasduction pathways by detecting translocations of factors of interest between the two fractions by ELISA, Western blot or other assays.

Supplier: Cayman Chemical

A 96-well assay for measurement of CREB.

Supplier: Biotium

Apoptosis and necrosis are two processes by which cells die. Apoptosis is an active, regulated disassembly of the cell from within. During apoptosis, phosphatidylserine is translocated from the inner to the outer surface of the cell, allowing the dying cell to be engulfed by phagocytic cells. Annexin V is a 35 kD Ca2+-dependent phospholipid binding protein with a high affinity for phosphatidylserine.

Supplier: Merck

This Kit overcomes the limitations of traditional multiwell migration assays. Its microfluidic, low-volume technology promotes a stable, diffusion-generated concentration gradient that is consistently linear and lasts for more than 48 hours.

Supplier: ENZO LIFE SCIENCES

MITO-ID® membrane potential detection kit monitors energetic status using a simple mix-and-read, no-wash protocol. It measures mitochondrial membrane potential with a cationic dye that fluoresces either green or orange depending upon membrane potential status.

Supplier: Cayman Chemical

Cholesterol Cell-Based Detection Assay Kit is a 192-well assay for measurement of cholesterol.

Supplier: Cayman Chemical

A 96-well assay for measurement of PPARα DNA binding activity.

Supplier: Biotium

JC-1 dye is used for fluorescence-based measurement of mitochondrial membrane potential changes in cells, a key measure of cell viability.

Supplier: Biotium

MTT, XTT, and resazurin are reduced by mitochondrial metabolic activity to yield coloured or fluorescent products, and thus are useful for and assaying cell viability and quantitating cell number. MTT and XTT are reduced to coloured formazin salts that can be measured by absorbance. MTT generates an insoluble formazin salt, requiring cell lysis before the absorbance can be measured, while XTT does not require cell lysis for measurement. Resazurin is a non-fluorescent blue dye that is reduced to the pink fluorescent compound resorufin, which can be measured by fluorescence or absorbance.

Supplier: Cayman Chemical

A 96-well assay for measurement of SREBP-1.

Supplier: ENZO LIFE SCIENCES

For use with GFP-expressing cell lines, as well as cells expressing blue, cyan or yellow fluorescent proteins.