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Stains

Overcome normal optical limitations in microscopic studies by highlighting specific structures with stains and reagents and then protect samples with fixatives. The fast-acting dyes create strong contrasts between cell components for easier detection or analyses. Sensitive attractions to certain organelle or cellular components differ among offered stains, allowing precise testing modifications. Flexible in use, ready-to-use stains may be handled manually or in conjunction with automatic equipment.

Electrophoresis gel stain, Reversible Copper Stain™

Electrophoresis gel stain, Reversible Copper Stain™

Supplier: G-Biosciences

The Reversible Copper Stain™ is a single step stain for the rapid detection of proteins resolved on SDS-PAGE.

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Electrophoresis gel stain, Reversible Zinc Stain™

Electrophoresis gel stain, Reversible Zinc Stain™

Supplier: G-Biosciences

A single step stain for the rapid detection of proteins resolved by PAGE. The stain is based on the interaction of zinc ions with polyacrylamide and proteins.

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VWR® BANDit™ Protein Stain Blue

VWR® BANDit™ Protein Stain Blue

Supplier: VWR Chemicals

Blue BANDit is a safe and environmentally friendly alternative to traditional Coomassie® Blue staining.

    
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Glycoprotein stain

Glycoprotein stain

Supplier: G-Biosciences

The stain uses an enhanced Periodic Acid-Schiff (PAS) method for detection of glycoprotein sugars. The supplied agent first oxidises the cis-diol sugar groups to aldehydes. The aldehyde groups react with the sensitive glycoprotein stain solution forming Schiff bonds and producing strong magenta colour bands. RAPIDstain™ can be used after glycoprotein staining to detect non-glycosylated proteins and the use of the stain enhances glycoprotein staining. The glycoprotein stainis highly convenient as all the key reagents required for staining are supplied and a unique positive & negative control is included.

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VWR® VividPro™ Fluorescent Stains

VWR® VividPro™ Fluorescent Stains

Supplier: VWR Chemicals

VividPro™ is a generic fluorescent stain for protein gel electrophoresis that can be used to visualize proteins.

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VWR® VividPro™ Plus Fluorescent Protein Stains

VWR® VividPro™ Plus Fluorescent Protein Stains

Supplier: VWR Chemicals

VividPro Plus™ is a fluorescent protein stain that can be used in a fast co-electrophoretic protocol to visualize proteins in SDS PAGE gels.

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Silver stain, FOCUS™ FASTsilver™

Silver stain, FOCUS™ FASTsilver™

Supplier: G-Biosciences

FOCUS™ FASTsilver™ produces crystal clear backgrounds and maximal peptide recovery needed for critical analysis by mass spectrometry. Complete proteolytic digestion and recovery of peptides is required for optimal analysis, however silver ions in traditional silver staining kits inhibit proteolytic digestion. In addition, glutaraldehyde, a common sensitiser in silver stains, modifies peptide lysine residues preventing complete digestion and recovery.

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Differentiation Solution

Supplier: SIGMA ALDRICH MICROSCOPY

Differentiation solution comprises acidified ethanol or isopropanol that is used to decolourise hematoxylin stain. The duration of exposure to the solution determines the degree of decolourisation. Differentiation is a crucial step in hematoxylin-eosin staining to achieve the proper colour contrast necessary for visualisation, by selectively removing excess hematoxylin from the tissue section and leaving behind the desired staining intensity.

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Aqueous mounting medium, Fluoromount™, Sigma-Aldrich®

Supplier: SIGMA ALDRICH MICROSCOPY

Fluoromount™ Aqueous Mounting Medium for use with fluorescent dye-stained tissue.

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Neutral mounting medium, DPX, Sigma-Aldrich®

Supplier: SIGMA ALDRICH MICROSCOPY

D.P.X mounting medium is a mixture of distyrene (polystyrene), a plasticizer (tricresyl phosphate), and xylene. It facilitates the protection of stains and can dry quickly.

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ImmunoHistoMount™ Mounting Medium

Supplier: SIGMA ALDRICH MICROSCOPY

ImmunoHistoMount™ is a permanent aqueous mounting medium designed for tissue sections and cell smears with peroxidase and alkaline phosphatase chromogens that cannot be dehydrated with organic solvents.

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CytoLiner™ Fixed Cell Membrane Stains

Supplier: Biotium

CytoLiner™ Fixed cell membrane dyes are novel lipophilic fluorescent dyes developed specifically for selective plasma membrane staining in fixed cells for microscopy. The dyes are engineered for robust and consistent staining of formaldehyde-fixed cells, and are suitable for downstream immunofluorescence staining protocols.

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Osteo-Bed Bone Embedding Solvent

Supplier: SIGMA ALDRICH MICROSCOPY

For use with Osteo-bed bone embedding kit. Solvent for removal of plastic from sections prior to rehydration and staining. Most histochemical and immunohistochemistry staining protocols require the removal of methyl methacrylate (MMA) and rehydration of mounted sections for improved stain penetration and greater contrast.

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Imperial™ Protein Stains

Imperial™ Protein Stains

Supplier: Thermo Fisher Scientific

Imperial protein stain is a ready-to-use colorimetric stain formulated with coomassie dye R-250 that delivers consistent nanogram-level detection of proteins in polyacrylamide electrophoresis gels or nitrocellulose membranes.

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Aqueous mounting medium Aquatex®, Sigma-Aldrich®

Supplier: Merck

Aquatex is used for e.g. LEUCOGNOST stained specimens. For preservation purposes and subsequent optimal microscopy, the stained sections are normally mounted using a suitable mounting agent and covered with a cover glass.

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Sigma-Aldrich® Bouin's solution

Supplier: SIGMA ALDRICH MICROSCOPY

Bouin's solution is an excellent fixative for preserving soft and delicate structures. It is prepared with saturated picric acid, formaldehyde and acetic acid.

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Protein gel stain, GelCode™ SilverSNAP® silver stain II, Pierce™

Protein gel stain, GelCode™ SilverSNAP® silver stain II, Pierce™

Supplier: Thermo Fisher Scientific

GelCode™ SilverSNAP® Silver Stain II is a fast, flexible stain that delivers consistently reliable staining performance.

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Non-aqueous DPX mounting medium

Supplier: Merck

DPX new is a synthetic non aqueous mounting medium for microscopy. In its formulation, the teratogenic plasticiizer dibutyl phthalate (DBP) has been avoided, which renders the composition safer for the environment and more user friendly than the conventionally used mounting agents such as DPX.

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M-FIX™ Spray fixative for gynecological and clinical cytology, Sigma-Aldrich®

M-FIX™ Spray fixative for gynecological and clinical cytology, Sigma-Aldrich®

Supplier: Merck

M-FIX is a fixative spray designed for the wet fixation of gynaecological and non-gynaecological preparations processed in Papanicolaou staining. It contains polyglycols in alcoholic solution and a rapid and secure fixation of specimens is possible.

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Immersion oil for microscopy, Sigma-Aldrich®

Supplier: Merck

Used to increase lens effectiveness

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Immersion oil for microscopy, Sigma-Aldrich®

Supplier: SIGMA ALDRICH MICROSCOPY

Immersion oil is used for high resolution (1000X) light microscopy work under oil immersion objective lens.

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Antifade mounting medium

Supplier: ENZO LIFE SCIENCES

Antifade mounting medium (hardset) is an antifade reagent that causes little or no quenching. Use of the mounting medium significantly extends the photobleaching half-life of the fluorescent stain.

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Mountant for histology, DPX, Sigma-Aldrich®

Supplier: SIGMA ALDRICH MICROSCOPY

DPX Mountant for histology has been used as a slide mountant to fix sections.

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J.T.Baker®, Cervix Fixative

Supplier: Avantor

Microscopy and staining reagents, J.T.Baker®, Cervix spray fixative

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DNA Staining System, FlashBlue™, Edvotek

Supplier: EDVOTEK

FlashBlue™ is a DNA stain that offers simple and rapid staining of agarose gels.

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Zinc Formalin Fixative

Supplier: SIGMA ALDRICH MICROSCOPY

Zinc formalin fixative is a non-precipitating fixative. This fixative is compatible with histochemical, immunochemical and special stains. It may be used with automated tissue processors and also in manual methods. The zinc chloride component of the formulation increases the rigidity of cellular components to withstand subsequent processing, sectioning and staining procedures. Tissue specimens initially fixed using 10% neutral buffered formalin solution may be post-fixed using the zinc formalin fixative.

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GelCode™ Blue Stain Reagent

GelCode™ Blue Stain Reagent

Supplier: Thermo Fisher Scientific

GelCode blue stain reagent is a ready-to-use protein stain based on colloidal coomassie dye G-250 that provides nanogram-level detection in polyacrylamide gels and for in-blot detection.

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PageBlue™ protein staining solution

Supplier: Thermo Fisher Scientific

PageBlue Protein Staining Solution is a sensitive, economical formulation of colloidal coomassie G-250 dye for endpoint staining of proteins in polyacrylamide gels and on PVDF membranes without methanol or acetic acid.

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EMBER™ Ultra DNA Gel Stain Kit

Supplier: Biotium

The most sensitive DNA gel stain available in convenient pre-coated agarose format with no need for post-electrophoresis staining.

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Hartman’s fixative histological tissue fixative, Sigma-Aldrich®

Supplier: SIGMA ALDRICH MICROSCOPY

Hartman′s fixative, which is also known as Davidson′s Fixative, contains acetic acid, alcohol, and formalin. It is widely used for the preservation of eye, bone marrow, breast, testis, and other tissues for histologic examination

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