Protein linking and labeling systems provide a convenient means for attaching fluorescent labels or biotins to antibodies and larger proteins. These kits are ideal for use with multiple applications, including flow cytometry, fluorescent microscopy, immunohistochemistry, primary detection, ELISAs, immunocytochemistry, and FISH. These systems are available with a variety of dye colors, biotin, fluorescent agents, and all the components needed to perform multiple conjugations and purifications. Protein labeling follows a simple protocol of reaction, separation, and then use.
VWR® Antibody signal enhancer
Supplier: VWR Chemicals
Antibody Signal Enhancer is a Western blotting antibody diluent that increases intensity of protein detection up to 5-fold compared to conventional blotting.
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SeramunBlock
Supplier: Apollo Scientific
Combined Stabiliser/ blocking solution for solid phase immobilised proteins effecting protein stabilisation and blocking of free surfaces.
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Antibody labelling kits, Mix-n-Stain™ CF™ Dye
Supplier: Biotium
Label your antibody with one of our superior CF® Dyes in 30 minutes without a purification step. Labelling tolerates many common buffer components including BSA and ascites.
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femtoCHROMO™- AP Substrate for chromogenic detection
Supplier: G-Biosciences
A ready to use modified BCIP (5-Bromo-4-Chloro-3’-Indolyphosphate p-Toluidine salt) and NBT (Nitro-Blue Tetrazolium Chloride) substrate that generates a black-purple insoluble precipitate in the presence of alkaline phosphatase.
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CF™ dye bovine serum albumin conjugates
Supplier: Biotium
Fluorescent bovine serum albumin (BSA) conjugates are commonly used as tracers in applications where physical dimensions are important. The conjugates can also be used for quantitative studies of electroporation, measurement of plasma volume in rats, and endocytosis studies. CF™ dye BSA conjugates are labelled with fluorescent CF™ dyes, which offer advantages in brightness and photostability compared to other fluorescent dyes.
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Amine reactive biotin reagents, HOOK™
Supplier: G-Biosciences
HOOK™ d-Biotin (Vitamin H), 500 mg
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Reactive biotin compounds
Supplier: Biotium
Biotium offers reactive biotin compounds with a variety of reactive functional groups.
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Antibody labelling kits, Mix-n-Stain™ Biotin
Supplier: Biotium
Mix-n-Stain™ Biotin antibody labelling kits utilise a technology that allows rapid antibody labelling with biotin without a purification step.
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CF™ Dyes Cholera Toxin Subunit B, CF™ Dye Conjugates
Supplier: Biotium
Cholera toxin subunit B conjugated to Biotium's next-generation fluorescent CF™ dyes.
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Annexin V conjugates
Supplier: Biotium
Annexin V fluorescent conjugates can be used to detect apoptotic cells. Annexin V is available conjugated to a wide variety of dyes.
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Stabiliser for calibrators and controls, SeramunStab® STD
Supplier: Apollo Scientific
Liquid product yellow odourless pH-value 7,3 to 7,5 Boiling point 102 °C. Stabiliser for complex mixtures of biomolecules.
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CF® Dye hydrazide
Supplier: Biotium
Fluorescent CF® Dye hydrazides are bright, extremely water-soluble, nontoxic molecules, useful as aldehyde-fixable cell tracers. They can also be used to label aldehyde or ketone groups of polysaccharides, carbohydrates or glycoproteins.
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CGH labeling kit, CYTAG®
Supplier: ENZO LIFE SCIENCES
Comparative genomic hybridisation (CGH) is a powerful diagnostic tool for detecting DNA copy number gains and losses associated with chromosome abnormalities. CGH provides an understanding of genetic disorders, cancers and other genomic aberrations.
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Stabiliser for sensitive biomolecules, SeramunStab® STB
Supplier: Apollo Scientific
Liquid product colourless to slightly yellow, freshly solutions opaleszent, odour weakly pH 7,3 to 7,5 boiling point 104 °C. Stabiliser for sensitive biomolecules like proteohormones or membrane proteins.
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NEDDylation kit
Supplier: ENZO LIFE SCIENCES
This kit provides the means of generating a thioester-linked NEDD8-conjugated E2 enzyme, utilizing the first two steps in the NEDD8 cascade, for use in the NEDDylation of E3 ligases and target substrate proteins (user supplied). A NEDD8-specific antibody is provided for detection of NEDDylated proteins via SDS-PAGE and Western blotting.
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Carboxyl coupling resin
Supplier: G-Biosciences
Carboxyl coupling resin consists of 6% cross-linked agarose with covalently linked diaminodipropylamine (DADPA) to generate a free primary amine at the end of a long spacer arm. Molecules, including proteins, peptides and nucleic acids, are covalently coupled to the free primary amines, and the stable columns are ideal for affinity purification of antibodies and other interacting partners.
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Tyramide amplification kit with HRP goat, anti-rabbit and CF® dye or biotin tyramide
Supplier: Biotium
Kits for increasing immunofluorescence sensitivity using HRP-catalysed tyramide signal amplification with the choice of next-generation fluorescent CF® dyes or biotin.
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Bio-N⁶-ddATP
Supplier: ENZO LIFE SCIENCES
Bio-N⁶-ddATP (Biotin-N⁶-(2’,3’-dideoxy)adenosine-5’ triphosphate) can be used for 3’-end labelling of single-stranded or double-stranded DNA. It is especially useful as a substrate for terminal transferase for the 3’-terminal addition of a single Bio-N⁶-ddAMP. It is also a substrate for Taq DNA Polymerase and other DNA polymerases.
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Bio-7-dATP
Supplier: ENZO LIFE SCIENCES
Bio-7-dATP (Biotin-7-2’-deoxyadenosine-5’-triphosphate) can replace dATP in reactions in which it serves as a substrate for E. coli DNA polymerase (holoenzyme and Klenow fragment), T4 and Taq DNA polymerases, reverse transcriptase (from AMV and M-MuLV) and terminal transferase.
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Allylamine-dUTP
Supplier: ENZO LIFE SCIENCES
Allylamine-2’-deoxyuridine-5’-triphosphate (Allylamine-dUTP) can replace TTP in reactions where it serves as a substrate for E.coli DNA polymerase 1 (holoenzyme and Klenow fragment), terminal deoxynucloetide transferase, T4 and Taq DNA polymerases, and reverse transcriptases (from ALV and MLV).
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Fluorescein-12-UTP
Supplier: ENZO LIFE SCIENCES
Fluorescein-12-UTP is ideal for in vitro transcription reactions catalysed by T3, T7, or SP6 RNA polymerases and reverse transcriptase to produce fluorophore-labeled RNA transcripts. RNA-based probes can be used to identify complementary sequences by in situ hybridisation to fixed cells and tissues by direct or indirect fluorescence detection or by membrane hybridisation procedures.
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Red 650 dUTP
Supplier: ENZO LIFE SCIENCES
Red 650 [Cyanine-5E] dUTP is a cyanine-5 derivative with enhanced spectral properties. This nucleotide analog can replace TTP in reactions in which it serves as a substrate for E.coli DNA polymerase (holoenzyme and Klenow fragment), T4 and Taq DNA polymerases, reverse transcriptase (from AMV and M-MuLV) and terminal transferase. Fluorescently labeled probes can be prepared with this fluorescent nucleotide by a variety of methods including nick translation, cDNA labelling and 3'-end labelling.
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Phosphothreonine detection set
Supplier: ENZO LIFE SCIENCES
This kit offers a set of three different monoclonal antibodies specific for threonine phosphorylation sites. Phosphorylation patterns in a given cell extract may differ when probed with different antibodies due to sequence specificity. Recognition therefore depends on two criteria: Phosphorylation and surrounding aa motif. If one of the two criteria is not fulfilled, the antibody will not detect the phosphorylation site.
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Phalloidin conjugates
Supplier: Biotium
Phalloidin is a toxin isolated from the deadly Amanita phalloides mushroom. It is a bicyclic peptide that binds specifically to F-actin. Fluorescent conjugates of phalloidin are convenient tools to investigate the distribution of F-actin in fixed and permeabilised cells or tissue sections. One unit (U) of phallodin is sufficient to stain one sample (200 µl staining volume). A highly selective actin filament stain for fixed and permeabilised cells. Labeled phalloidins have similar affinity for both large and small filaments.
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Phosphotyrosine detection set
Supplier: ENZO LIFE SCIENCES
This kit offers a set of seven different MAbs specific for tyrosine phosphorylation sites. Phosphorylation patterns in a given cell extract may differ when probed with different antibodies due to sequence specificity. Recognition therefore depends on two criteria: Phosphorylation and surrounding aa motif. If one of the two criteria is not fulfilled, the antibody will not detect the phosphorylation site. Specificity has been determined by epitope mapping using degenerated phosphopeptide libraries.
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AMC (7-Amino-4-methylcoumarin) standard
Supplier: ENZO LIFE SCIENCES
Fluorogenic chromophore. Ex(max): 380nm, Em(max): 460nm. Reference compound.
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Nuclear-ID™ Blue/Red cell viability reagent (GFP-Certified™)
Supplier: ENZO LIFE SCIENCES
Cell viability dye
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femtoCHROMO™- HRP Substrate for chromogenic detection
Supplier: G-Biosciences
A ready to use modified TMB (3, 3’, 5, 5’-Tetramethylbenzidine) substrate is used that generates a dark blue precipitate in the presence of horseradish peroxidase.