You searched for: Buffers
Resisting pH changes, buffers protect samples when acids or alkali is added. Powder and liquid forms efficiently track proteins during electrophoresis isolation and separation. The salty buffers can also be used to calibrate and standardize pH meters for accurate readings. Blocking substances stop antibody attachment to membrane free sites that would produce an interfering background. In various concentrations to fit any need, the buffers may come in liquids or dehydrated formats.
Phosphate buffered saline (PBS), 10X
Supplier: G-Biosciences
PBS 10X solution is used as a wash buffers for Western blotting, ELISA and other applications.
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Proteasome assay buffer
Supplier: ENZO LIFE SCIENCES
Assay buffer from the 20S Proteasome Assay Kit for Drug Discovery.
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Buffer, TAE solution 50X concentrate (TRIS-acetate-EDTA buffer)
Supplier: Thermo Fisher Scientific
Running Buffer, TAE buffer solution 50X concentrate (TRIS-acetate-EDTA buffer)
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Buffer, TRIS solution pH 8 (1 mol/L)
Supplier: Cayman Chemical
Cloning Buffer, TRIS buffer solution pH 8 (1 mol/L)
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Buffer, EDTA buffer solution pH 8,0 (0,5 mol/l)
Supplier: Thermo Fisher Scientific
Cloning Buffer, EDTA buffer solution pH 8,0 (0,5 mol/l)
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Wash buffer concentrate
Supplier: ENZO LIFE SCIENCES
Liquid. TRIS buffered saline containing detergents.
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Buffer, EDTA buffer solution pH 8,5 (0,5 mol/L)
Supplier: G-Biosciences
A DNase and RNase free solution of 0.5M EDTA.
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Buffer, TRIS solution pH 7,5 (1 mol/L)
Supplier: Cayman Chemical
Cloning Buffer, TRIS buffer solution pH 7,5 (1 mol/l)
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Buffers, TE solutions 10X concentrates (TRIS-EDTA buffer)
Supplier: Rockland Immunochemicals
TE buffer is a commonly used buffer solution in molecular biology, especially in procedures involving DNA, cDNA or RNA. "TE" is derived from its components: Tris, a common pH buffer, and EDTA, a molecule that chelates cations like Mg2+. The purpose of TE buffer is to solubilize DNA or RNA, while protecting it from degradation.
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J.T.Baker® BAKER ANALYZED®, Buffer Solutions, Colour Coded
Supplier: Avantor
The quality of many BAKER ANALYZED™ reagents meets or exceeds the requirements set forth by the American Chemical Society (ACS).
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Permeabilisation buffer
Supplier: Biotium
Ready-to-use permeabilisation buffer for intracellular immunofluorescence staining for microscopy or flow cytometry.
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VWR® TISAB II Solution (Total Ionic Strength Adjustment Buffer) for Fluoride Determinations
Supplier: VWR Chemicals
Total ionic strength adjustment buffer solution for use with selective ion electrodes.
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Hybridisation buffers
Supplier: G-Biosciences
The hybridisation buffers like Church & Gilbert’s, Denhardt solution, hybridisation denaturing solution, hybridisation neutralising solution, SSC, SSPE, TMAC Hyb solution and TNT Buffer are used in wide range of applications. Church & Gilbert’s Hybridization Buffer is prepared from molecular biology grade SDS (sodium dodecyl sulfate), monosodium phosphate (NaHPO₄), EDTA (ethylenediaminetetracetic acid) and bovine serum albumin (BSA) Fraction V using DNase, RNase and Protease-free water. It consists of 1mM EDTA, 1% BSA, 0,5M sodium phosphate monobasic, and 7% SDS (at a pH of 7,2).
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Cesium nitrate, ionization buffer solution, Specpure®
Supplier: Thermo Fisher Scientific
Cesium Nitrate Buffer solution
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Buffer, SSC solution 20X concentrate (saline-sodium citrate)
Supplier: G-Biosciences
Hybridisation Buffer, SSC buffer solution 20X concentrate (saline-sodium citrate)
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Blocking buffers, FirstChoice™
Supplier: G-Biosciences
FirstChoice™ Blocking Buffer is ideal as a first choice for optimization of new assays and systems, or when determining the optimal blocking buffer for elimination of non specific binding sites in ELISA, blotting, immunohistochemistry and other applications. Buffers are conveniently supplied in widely used TBS (Tris-buffered saline at pH 7,5) and PBS (phosphate-buffered saline at pH 7,5) buffers as well as in separate formulations containing Tween® 20 for improving blocking efficiencies.
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VWR®RapidBlock™, Blocking solution, 10x
Supplier: VWR Chemicals
RapidBlock™ solution reduces blocking time to five minutes for Western and dot blotting procedures on PVDF and nitrocellulose membranes. This protein-free formulation minimises cross-reactivity and non specific antibody binding, generating blots with low background and enhanced signal-to-noise ratio.
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Buffer, triethylammonium acetate, 1 M solution, pH 7,0
Supplier: PanReac AppliChem
Used for the purification of oligonucleotides.
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Buffer, HEPES solution (1 mol/L)
Supplier: Cayman Chemical
HEPES buffer solution (1 mol/l)
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Guanidine hydrochloride buffer solution, 6 mol/L, pH 8,7
Supplier: TCI
Guanidine Hydrochloride Buffer Solution, 6 mol/L, pH value: 8,7, TCI
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Lithium nitrate, ionisation buffer solution, Specpure®
Supplier: Thermo Fisher Scientific
Lithium Nitrate Buffer Solution
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Fixation buffer for microscopy and flow cytometry
Supplier: Biotium
Ready-to-use formaldehyde-based fixation buffer for immunofluorescence staining for microscopy or flow cytometry.
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Buffer, EDTA buffer solution pH 8,0 (0,5 mol/L)
Supplier: Rockland Immunochemicals
EDTA is widely used for scavenging metal ion in biochemistry and molecular biology. Ion depletion is commonly used to deactivate metal-dependent enzymes, either as an assay for their reactivity or to suppress damage to DNA or proteins.
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Protein Transfer buffers
Supplier: G-Biosciences
Efficient™ Western Transfer Buffer (20X) achieves increased protein transfer efficiency. This ready-to-use transfer buffer is prepared for optimal conductivity and efficient protein transfer, without generating excessive heat or transfer distortion.
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Protein blocking buffers, Superior™
Supplier: G-Biosciences
Blocking buffer is an enhanced blocking agent available in multiple formats. Superior™ Blocking Buffer contains a proprietary antigenically non-determinant protein for blocking Western blot membranes, blocking and storage of ELISA plates, and blocking prior to immunohistochemistry staining.
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Cathepsin S Assay Buffer
Supplier: ENZO LIFE SCIENCES
50mM potassium phosphate buffer, pH 6,5, 50 mM NaCl, 2 mM EDTA, 0,01% Triton X-100®, 0,5 mM DTT.
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Mini flip-top vial with 9 ml Butterfield's buffer
Supplier: 3M Food Safety
The 3M™ Mini Flip-Top Vial with 9 ml Butterfield's Buffer is a leak-resistant plastic cylinder with a flip-open lid that seals securely for safe shipping and handling.
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Caspase cell lysis buffer
Supplier: ENZO LIFE SCIENCES
Caspase cell lysis buffer, Enzo Life Sciences
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6X GelRed prestain loading buffer with tracking dye
Supplier: Biotium
6X GelRed prestain loading buffers are gel loading buffers containing density agents, tracking dyes, and GelRed dye. GelRed is a sensitive, stable and environmentally safe fluorescent nucleic acid dyes designed to replace the highly toxic ethidium bromide (EtBr).