Buffers
Resisting pH changes, buffers protect samples when acids or alkali is added. Powder and liquid forms efficiently track proteins during electrophoresis isolation and separation. The salty buffers can also be used to calibrate and standardize pH meters for accurate readings. Blocking substances stop antibody attachment to membrane free sites that would produce an interfering background. In various concentrations to fit any need, the buffers may come in liquids or dehydrated formats.
Krebs-Ringer solution, bicarbonate-buffered
Supplier: Thermo Fisher Scientific
Contains: 120 mM sodium chloride, 5 mM potassium chloride, 2 mM calcium chloride, 1 mM magnesium chloride, 25 mM sodium bicarbonate, and 5,5 mM D-glucose.
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Phosphate, 0,5M buffer solution, pH 7,5
Supplier: Thermo Fisher Scientific
Phosphate buffer soln, 0,5 M, pH value: 7,5, Liquid
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HEPES, 1,0 M buffer solutions
Supplier: Thermo Fisher Scientific
HEPES buffer solution, 1 mol/L, pH value: 9,0, Liquid
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MES-SDS running buffer (20X)
Supplier: Thermo Fisher Scientific
Running Buffer, MES-SDS running buffer (20X)
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Phosphate-buffered saline (PBS, 10X), pH 7,6
Supplier: Thermo Fisher Scientific
Cell culture buffer, PBS, 10 X
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Buffer, potassium phosphate, 0,2 M solution
Supplier: Thermo Fisher Scientific
Potassium phosphate, 0,2M buffer solution, pH 8,0, Liquid
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MOPS-SDS running buffer (20X)
Supplier: Thermo Fisher Scientific
Running Buffer, MOPS-SDS running buffer (20X)
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TE buffer, pH 7,4, RNAse free
Supplier: Thermo Fisher Scientific
TE buffer is a commonly used buffer solution in procedures involving DNA or RNA. It is used for storing and diluting DNA and RNA. Treatment with TE buffer enhances the staining intensity of antibodies in the immunohistochemical detection of certain proteins.
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TRIS-buffered saline (TBS, 10X), with 1% Triton® X-100
Supplier: Thermo Fisher Scientific
Washing buffer, TRIS-buffered saline (TBS, 10X), with 1% Triton® X-100
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TRIS-buffered saline (TBS, 10X) pH 7,4
Supplier: Thermo Fisher Scientific
Washing buffer, TRIS-buffered saline (TBS, 10X)
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Kinase buffer II (5X)
Supplier: Thermo Fisher Scientific
Kinase buffer II (5X)
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Buffers, glycylglycine, 0,2 M solutions
Supplier: Thermo Fisher Scientific
Glycylglycine, buffer solution, 0,2 M, pH value: 8,5, Colourless, Odourless, Liquid
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TE buffer, pH 8,1, RNAse free
Supplier: Thermo Fisher Scientific
Trizma is used in the formulation of buffer solutions in the pH range between 7,5 and 8,5. Tris buffer solutions are widely used in cell and molecular biology for processes such as protein and nucleic acid extraction and purification.
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Sodium bicarbonate, Corning®
Supplier: Corning
Sodium bicarbonate is used as part of a buffering system commonly used to maintain physiological pH 7,2 to 7,4 in a culture environment
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Alkaline running buffer solution
Supplier: Thermo Fisher Scientific
Running Buffer, Alkaline running buffer solution
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Supplier: VWR Chemicals
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