Buffers

Resisting pH changes, buffers protect samples when acids or alkali is added. Powder and liquid forms efficiently track proteins during electrophoresis isolation and separation. The salty buffers can also be used to calibrate and standardize pH meters for accurate readings. Blocking substances stop antibody attachment to membrane free sites that would produce an interfering background. In various concentrations to fit any need, the buffers may come in liquids or dehydrated formats.

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Tris-HCl buffer, pH 10, 10×, antigen retriever, Sigma-Aldrich®

Supplier: SIGMA ALDRICH MICROSCOPY

Intended for heat-induced antigen retrieval on formalin-fixed paraffin-embedded (FFPE) tissue sections prior to application of antibodies in immunohistochemistry (IHC). Improves accessibility of antibodies to tissue antigens.

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VWR®, Tris buffer solution, pH 8 (1 mol/L) for biotechnology

Supplier: VWR Chemicals

Tris (hydroxymethyl) aminomethane.

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VWR® Buffer Tablets for Haematology

Supplier: VWR Chemicals

These tablets provide a rapid and convenient method of preparing a buffer solution.

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J.T.Baker® DILUT-IT™, Buffer Solutions, Concentrate

Supplier: Avantor

Volumetric concentrate, for dilution to 500 ml.

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VWR®, MOPS 10X buffer for biotechnology

Supplier: VWR Chemicals

MOPS is a zwitterionic buffer used as a running buffer for denaturing agarose gel electrophoresis of RNA. Having a pH buffering range from 6,5 to 7,9, MOPS works exceptionally well with formaldehyde gels at 20 mM concentration.

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J.T.Baker® Cell Lysis Solution, Biotech Reagent

Supplier: Avantor

J.T.Baker® Cell Lysis Solution Biotech Reagent is a cGMP pre-formulated and ready to use detergent optimized for the extraction and recovery of AAV particles from producer cell lines (HEK293 parental and suspension lines, SF9). J.T.Baker® Cell Lysis Solution is a proprietary blend of nonionic surfactants, free of octyl phenol ethoxylate, which demonstrates comparable performance industry standard detergents while addressing environmental concerns associated with REACH and downstream clearance concerns. Designed for Gene Therapy workflows, this formulation is easy to use and handle as it is ready-to-use, has low viscosity, does not require cold temperature shipping or storage, and is available in sizes to support processes from development to large scale commercial batches. Detail description provided in technical literature.

Sustainable Options Available

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J.T.Baker® DILUT-IT™, Sodium Phosphate, Dissolution Media Concentrate

Supplier: Avantor

DILUT-IT™ dissolution media concentrate, sodium phosphate, 961,5 ml, J.T.Baker® DILUT-IT®, pH value: 6,8

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Buffer solutions, Certipur®, colour coded, Supelco®

Supplier: Merck

Materials for the precise calibration and monitoring of pH meters.

Sustainable Options Available

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TISAB III solution (Total Ionic Strength Adjustment Buffer) for fluoride determinations, Supelco®

Supplier: Merck

TISAB III solution for fluoride determinations

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VWR® Buffer Solutions, Secondary Standards

Supplier: VWR Chemicals

Buffer solutions, secondary standards, pH value: 7,413, ±0,010, Reference temperature: 25 °C, VWR®, Plastic bottle

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VWR®, SSC buffer, 20X, ultra-pure grade

Supplier: VWR Chemicals

SSC buffer is a hybridisation buffer for Northern and Southern blots.

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VWR®, Eluent buffers for LC

Supplier: VWR Chemicals

LC eluent C, LUFA

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VWR® UltraPure, Phosphate buffered saline (PBS), powdered

Supplier: VWR Chemicals

PBS, Ultra Pure Grade is a powdered, magnesium- and calcium-free formulation of PBS suitable for cell culture and for use in flow cytometry applications.