Buffers

Resisting pH changes, buffers protect samples when acids or alkali is added. Powder and liquid forms efficiently track proteins during electrophoresis isolation and separation. The salty buffers can also be used to calibrate and standardize pH meters for accurate readings. Blocking substances stop antibody attachment to membrane free sites that would produce an interfering background. In various concentrations to fit any need, the buffers may come in liquids or dehydrated formats.

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Buffer concentrate, citrate phosphate, Sigma-Aldrich®

Supplier: SIGMA ALDRICH MICROSCOPY

These buffers are used to elute adult hemoglobin by the procedure of Oski and Naiman, a modification of the Kleihauer Betke procedure for the demonstration of fetal hemoglobin.

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VWR®, Tris buffer solution, pH 8 (1 mol/L) for biotechnology

Supplier: VWR Chemicals

Tris (hydroxymethyl) aminomethane.

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VWR® Buffer Tablets for Haematology

Supplier: VWR Chemicals

These tablets provide a rapid and convenient method of preparing a buffer solution.

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VWR®, MOPS 10X buffer for biotechnology

Supplier: VWR Chemicals

MOPS is a zwitterionic buffer used as a running buffer for denaturing agarose gel electrophoresis of RNA. Having a pH buffering range from 6,5 to 7,9, MOPS works exceptionally well with formaldehyde gels at 20 mM concentration.

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J.T.Baker® Cell Lysis Solution, Biotech Reagent

Supplier: Avantor

J.T.Baker® Cell Lysis Solution Biotech Reagent is a cGMP pre-formulated and ready to use detergent optimized for the extraction and recovery of AAV particles from producer cell lines (HEK293 parental and suspension lines, SF9). J.T.Baker® Cell Lysis Solution is a proprietary blend of nonionic surfactants, free of octyl phenol ethoxylate, which demonstrates comparable performance industry standard detergents while addressing environmental concerns associated with REACH and downstream clearance concerns. Designed for Gene Therapy workflows, this formulation is easy to use and handle as it is ready-to-use, has low viscosity, does not require cold temperature shipping or storage, and is available in sizes to support processes from development to large scale commercial batches. Detail description provided in technical literature.

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J.T.Baker® DILUT-IT™, Buffer Solutions, Concentrate

Supplier: Avantor

Volumetric concentrate, for dilution to 500 ml.

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J.T.Baker® DILUT-IT™, Sodium Phosphate, Dissolution Media Concentrate

Supplier: Avantor

DILUT-IT™ dissolution media concentrate, sodium phosphate, 961,5 ml, J.T.Baker® DILUT-IT®, pH value: 6,8

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TISAB solution for Fluoride Determination, Supelco®

Supplier: Merck

TISAB solution for fluoride determination

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Cesium chloride-aluminium nitrate buffer solution acc. to Schuhknecht and Schinkel, Supelco®

Supplier: Merck

Cesium chloride-aluminium nitrate buffer solution acc. to Schuhknecht and Schinkel for the determination of sodium, potassium and lithium by flame spectrometry (enth./cont. 50 g/l CsCl + 250 g/l Al(NO₃)₃ * 9 H₂O)

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Citrate buffer, pH 6.0, 10×, antigen retriever, Sigma-Aldrich®

Supplier: SIGMA ALDRICH MICROSCOPY

Citrate buffer, antigen retriever has been used as a heat-induced antigen retriever on formalin-fixed paraffin-embedded (FFPE) tissue sections prior to application of antibodies or DNA fluorescent in situ hybridization.

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Tris-HCl buffer, pH 10, 10×, antigen retriever, Sigma-Aldrich®

Supplier: SIGMA ALDRICH MICROSCOPY

Intended for heat-induced antigen retrieval on formalin-fixed paraffin-embedded (FFPE) tissue sections prior to application of antibodies in immunohistochemistry (IHC). Improves accessibility of antibodies to tissue antigens.

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EDTA Buffer, pH 8,5, 10X, Antigen Retriever

Supplier: SIGMA ALDRICH MICROSCOPY

Used as a heat-induced antigen retriever on formalin-fixed paraffin-embedded (FFPE) tissue sections prior to application of antibodies.

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Buffer solutions in sachets, Certipur®, Supelco®

Supplier: Merck

For calibration of pH meters.

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Buffer solutions, Certipur®, ready to use, 1 litre bottles, Supelco®

Supplier: Merck

Materials for the precise calibration and monitoring of pH meters.

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TISAB II Solution, Supelco®

Supplier: Merck

TISAB, in general, is mixed with samples, as it helps in maintaining the ionic strength and pH constant. It is mainly used to decomplex metal-F complexes.