You searched for: qPCR/RT-qPCR Enzymes and Kits
One-Step SYBR® Green qRT-PCR, qScript™
Supplier: Quantabio
qScript™ One-Step SYBR® Green qRT-PCR Kits reliably deliver maximum qRT-PCR efficiency, sensitivity and specificity in with a simplified protocol. These kits offer reliable and unbiased amplification of all RNA targets regardless of abundance. The proprietary supermix maximises activities of both reverse transcriptase and AccuStart™ Taq DNA polymerase without compromising specificity of amplification. qScript™ One-Step SYBR® Green qRT-PCR kits are available and optimised for all real-time PCR instrument platforms including those requiring normalisation with ROX reference dye.
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AMPIGENE® qPCR 1-Step Probe Kit Lo-ROX
Supplier: ENZO LIFE SCIENCES
Optimised and efficient cDNA synthesis and probe-based qPCR in a single tube.
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Brilliant III Ultra-Fast SYBR® green ROX QPCR master mix
Supplier: AGILENT
High performance, ultra-sensitive, SYBR® Green QPCR master mix reagent with ROX concentration for reliable quantification across a wide range of targets and templates.
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Fast probe master mixes
Supplier: Biotium
A qPCR master mix containing Cheetah™ Taq hotstart DNA polymerase, suitable for probe-based real-time PCR applications.
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MVP human total RNA
Supplier: AGILENT
MVP RNA is application-ready RNA available in small economical pack sizes.
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AMPIGENE® qPCR 1-Step Green Kit Hi-ROX
Supplier: ENZO LIFE SCIENCES
Optimised and efficient cDNA synthesis and probe-based qPCR in a single tube.
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PerfeCTa® QPCR mixes, SYBR® green super mixes and fast mixes
Supplier: Quantabio
PerfeCTa® SYBR® Green SuperMixes and FastMixes™ enable efficient, sensitive and precise quantitative PCR with proprietary buffers and SYBR® Green stabilisers that maximise fluorescent signal, PCR efficiency, and reduce primer dimers. These SuperMixes and FastMixes™ have been optimised for all Real-Time PCR instrument platforms, including those requiring normalisation with ROX reference dye or fluorescein.
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One-Step Fast qRT-PCR kits, qScript™
Supplier: Quantabio
These kits offer a convenient and highly sensitive solution for Real-Time reverse transcriptase PCR (qRT-PCR) of RNA templates using hybridisation probe detection chemistries, such as TaqMan® 5'-hydrolysis probes.
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AMPIGENE® qPCR 1-Step Probe Kit No-ROX
Supplier: ENZO LIFE SCIENCES
Optimised and efficient cDNA synthesis and probe-based qPCR in a single tube.
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Taq extender PCR additive
Supplier: AGILENT
Improves reliability and yield of conventional Taq-based PCR amplifications.
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Brilliant III Ultra-fast probe ROX qPCR master mix
Supplier: AGILENT
High performance, ultra-sensitive, probe QPCR master mix reagent with high or low ROX concentration for reliable quantification across a wide range of targets and templates.
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Viability PCR starter kits
Supplier: Biotium
Kits designed to make it simple to start performing viability PCR with the choice of viability dye (PMA or PMAxx™).
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qScript™ XLT One-Step RT-qPCR ToughMix®
Supplier: Quantabio
qScript™ XLT One-Step RT-qPCR ToughMix® is a ready to use, highly sensitive master mix for reverse transcription quantitative PCR (RT-qPCR) of RNA templates.
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AMPIGENE® qPCR 1-Step Probe Kit Hi-ROX
Supplier: ENZO LIFE SCIENCES
Optimised and efficient cDNA synthesis and probe-based qPCR in a single tube.
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qPCR SuperMix, PerfeCTa® MultiPlex
Supplier: Quantabio
This SuperMix is a 2X concentrated, ready to use reaction cocktail for Real-Time qPCR that contains all components, except primers, probes, and templates. The system transcends multiplex limitations of conventional PCR master mixes, enabling unbiased amplification of up to five target sequences in a single tube. Suppression of low copy amplicons by high copy reference targets in the amplification is a common problem in multiplex PCR. This can skew, or mask the apparent representation and quantification of low copy target sequences. PerfeCTa® MultiPlex qPCR SuperMix delivers dynamic range and sensitivity to multiplexed qPCR that is comparable to that for singleplex qPCR probe assays without the need for limiting or variable primer concentrations.
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PerfeCTa® qPCR ToughMix®
Supplier: Quantabio
PerfeCTa® qPCR ToughMix® is a 2X concentrated ready to use reaction cocktail for PCR amplification of DNA templates that overcomes many known inhibitors of PCR often present in crude samples extracted from environmental specimens, plant tissues or animal tissues. It is a versatile and robust Real-Time qPCR reagent that provides maximum sensitivity and PCR efficiency with a variety of fluorogenic probe chemistries, including TaqMan® hydrolysis probes. PerfeCTa® qPCR ToughMix® contains all required reaction components, except primers, probe(s) and DNA template. The light blue colour of the AccuVue™ tracer dye simplifies reaction assembly in white, or clear plates and helps to minimise pipetting or mixing errors. It does not interfere with qPCR performance or affect the stability of the product.
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Brilliant III Probe MM, Agilent Technologies
Supplier: AGILENT
Brilliant III QPCR and QRT-PCR master mixes increase overall sensitivity and speed through the novel, quick-activating hot start and the engineered Taq-mutant.
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Brilliant II probe MM
Supplier: AGILENT
Brilliant II QPCR and QRT-PCR reagents were developed for improved sensitivity of detection, ensuring reproducible quantification even at low target concentrations.
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Brilliant II SYBR® Master mix
Supplier: AGILENT
Brilliant II SYBR® QPCR and QRT-PCR reagents were developed for improved sensitivity of detection, ensuring reproducible quantification even at low target concentrations.
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Luminaris color Probe qPCR master mixes
Supplier: Thermo Fisher Scientific
Luminaris Color Probe qPCR Master Mixes are universal ready-to-use solutions optimised for qPCR and two-step RT-qPCR. The master mixes include hot start Taq DNA polymerase, uracil-DNA glycosylase (UDG) and dNTPs in an optimised PCR buffer. Only the template, primers and probe need to be added.
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Maxima™ Probe qPCR Master Mix (2X)
Supplier: Thermo Fisher Scientific
The Maxima™ Probe qPCR Master Mix (2X) is a ready to use solution optimised for quantitative real time PCR and two-step real time RT-PCR on most real time PCR machines. The master mix includes Maxima™ hot start Taq DNA polymerase and dNTPs in an optimised PCR buffer. dUTP is included in the mix for optional carryover contamination control using uracil-DNA glycosylase (UDG). The use of Maxima™ probe qPCR master mix (2X) in real time PCR ensures reproducible, sensitive and specific quantification of genomic, plasmid, viral and cDNA templates.
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ROX Dye qPCR Calibration Solution *10000X*
Supplier: AAT BIOQUEST
ROX dye qPCR Calibration Solution supplied as a 10000X concentrate is compatible with a wide variety of qPCR instruments, including the ABI7500 Fast, QuantStudio™, and ViiA™ 7 systems.
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DyNAmo™ ColorFlash SYBR® green QPCR kits
Supplier: FINNZYMES REAGENTS
DyNAmo™ qPCR Kits offer excellent performance in detection and quantitation of DNA and RNA sequences from various sources. The DyNAmo™ qPCR kit family features optimised kits for fast and conventional qPCR. Kits utilising either SYBR® Green or probe chemistries for various platforms are available. All DyNAmo™ qPCR kits are provided as convenient 2X master mixes - only template and primers need to be added.
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Maxima™ SYBR® green QPCR master mix (2X), with ROX
Supplier: Thermo Fisher Scientific
The Maxima™ SYBR® Green qPCR Master Mix (2X) is a ready to use solution optimised for quantitative real time PCR and two-step real time RT-PCR on most real time PCR machines. The master mix includes Maxima™ hot start Taq DNA polymerase, SYBR® Green dye I and dNTPs in an optimised PCR buffer. ROX solution is provided in a separate vial for use with machines that require ROX as a passive reference dye. Maxima™ hot start Taq DNA polymerase in combination with the optimised buffer ensures PCR specificity and sensitivity. The SYBR® Green I intercalating dye allows for DNA detection and analysis without using sequence specific probes. dUTP is included in the mix for optional carryover contamination control using uracil-DNA glycosylase (UDG). The use of Maxima™ SYBR® Green qPCR Master Mix (2X) in real time PCR ensures reproducible, sensitive and specific quantification of genomic, plasmid, viral and cDNA templates.
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TAQuest™ FAST qPCR Master Mix with Helixyte™ Green *No ROX*
Supplier: AAT BIOQUEST
TAQuest™ FAST qPCR Master mix with Helixyte™ green is a ready-to-use 2X solution optimised for qPCR and two-step RT-qPCR.
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TAQuest™ FAST qPCR Master Mix with Helixyte™ Green *High ROX*
Supplier: AAT BIOQUEST
TAQuest™ FAST qPCR Master mix with Helixyte™ green is a ready-to-use 2X solution optimised for qPCR and two-step RT-qPCR.
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TAMRA Dye qPCR Calibration Solution *10000X*
Supplier: AAT BIOQUEST
TAMRA dye qPCR Calibration Solution supplied as a 10000X concentrate is compatible with a wide variety of qPCR instruments, including the ABI7500 Fast, QuantStudio™, and ViiA™ 7 systems
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Q4ever™ Green
Supplier: AAT BIOQUEST
A real-time polymerase chain reaction (real-time PCR), also known as quantitative polymerase chain reaction (qPCR), is a popular laboratory technique of molecular biology based on the polymerase chain reaction (PCR).
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TAQuest™ FAST qPCR Master Mix for TaqMan Probes *Low ROX*
Supplier: AAT BIOQUEST
TAQuest™ FAST qPCR Master mix for TaqMan probes is a ready-to-use 2X solution optimised for qPCR and two-step RT-qPCR well suited to use for TaqMan gene expression assays.
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TAQuest™ qPCR Master Mix for TaqMan Probes *No ROX*
Supplier: AAT BIOQUEST
TAQuest™ qPCR Master mix for TaqMan probes is a ready-to-use 2X solution optimised for qPCR and two-step RT-qPCR compatible with TaqMan gene expression assays.