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Microscopy and staining reagents

Avantor offers a broad line of reagent droppers, diagnostic kits, and antigens and antisera, for aid in identifying a variety of microscopic organisms. The visualization of bacteria by means of staining procedures is an important tool for any microbiologist to have at their disposal, and these carefully standardized and reproducible stain reagents help to ensure accurate and easily interpretable results. Stains are also available to provide early detection of positive blood cultures by fluorescence procedures.

VWR® Q Path®, Medium-acting decalcifiers

VWR® Q Path®, Medium-acting decalcifiers

Supplier: VWR Chemicals

This medium-acting decalcifier contains a mixture of hydrochloric acids.

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VWR® Q path® Safesolv, Solvent Substitute

VWR® Q path® Safesolv, Solvent Substitute

Supplier: VWR Chemicals

Q Path® Safesolv is a non hazardous replacement for xylene or toluene. It is compatible with the Q Path® Safemount mounting medium reference 00647520. It is used in place of xylene, toluene, methylecyclohexane or other solvents in the course of the dehydration stages of histological specimens and during the stages of staining and lamella mounting.

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VWR® Q Path® Labofix Cytology Fixative

VWR® Q Path® Labofix Cytology Fixative

Supplier: VWR Chemicals

Q Path Labofix is a spray cytology fixative able to fix and store the cellular elements from gynaecological and non gynaecological smears after spreading them out on a glass slide.

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VWR® Q Path® Stick on (Cell adhesive) for Microscopy

VWR® Q Path® Stick on (Cell adhesive) for Microscopy

Supplier: VWR Chemicals

Glue for improving adherance of histologicals sections to slides. Alternative to albumin-glycerol.

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VWR® Q Path® Easyfix, Cytology Fixative

VWR® Q Path® Easyfix, Cytology Fixative

Supplier: VWR Chemicals

Cytology fixative specially designed for the conservation and transport of cytology specimens.

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Fluorescence antifade mounting media and CoverGrip™ coverslip sealant, EverBrite™

Fluorescence antifade mounting media and CoverGrip™ coverslip sealant, EverBrite™

Supplier: Biotium

EverBrite™ mounting media are anti-fade mountants for preserving fluorescence during microscopy. EverBrite™ mounting media prevent rapid photobleaching of fluorescein, rhodamine, and cyanine based dyes and are optimally formulated for use with Biotum’s CF™ dye conjugates. Unlike Vectashield® EverBrite™ mounting media is compatible with Cy® dyes such as Cy®2, Cy®3 and Cy®5. EverBrite™ mounting medium is a wet-set medium while EverBrite™ hardset mounting medium cures overnight to form a hard permanent seal with the coverslip, eliminating the need to seal coverslip edges with nail polish or other sealants. Both formulations are available with DAPI for blue fluorescent nuclear counterstaining without an additional staining step.

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VWR® Q Path® Freeze Gel (Glue) Mounting Medium for Cryotomy

VWR® Q Path® Freeze Gel (Glue) Mounting Medium for Cryotomy

Supplier: VWR Chemicals

High viscosity mounting media for cryotomy. Less viscous than O.C.T.

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Tissue-clearing reagent, CUBIC-X2 for animals

Supplier: TCI

Specifications
Appearance: Colorless to Light yellow to Light orange clear liquid
Functionality test: to pass test (Remaining fluorescence strength)

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Tissue-clearing reagent, TOMEI for plants

Supplier: TCI

Specifications
Appearance: Colorless to Light yellow clear liquid
Functionality test (transparency) (display): to pass test

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Embedding and mounting media

Embedding and mounting media

Supplier: Merck

The defined hardness and uniformity of the material to be cut are vital requirements in creating consistent quality in cutting. These requirements can be met using a range of suitable substances, such as paraffins, resins, gelatine and celloidin.

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VWR® Q Path® O.C.T. Compound Mounting Medium for Cryotomy

VWR® Q Path® O.C.T. Compound Mounting Medium for Cryotomy

Supplier: VWR Chemicals

High viscosity mounting medium for cryotomy.

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VWR® Q Path® Isomount 2000, Mounting Media

VWR® Q Path® Isomount 2000, Mounting Media

Supplier: VWR Chemicals

Mounting media based on isopropyl alcohol. Compatible with most solvent replacing agents. Suitable for manual and automatic mounting of slides. PET bottle of 500 ml.

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Water-free mounting medium, Neo-Mount®

Water-free mounting medium, Neo-Mount®

Supplier: Merck

Water-free mounting media, such as Neo-Mount®, should be used for permanent mounting and long-term storage of samples. Slides processed in pathologyl laboratories need to be stored up to 10 years, whereby the staining results need to be stable and fading-out is not acceptable. Neo-Mount®, anhydrous mounting medium for microscopy is an extremely colour-stable mounting medium for microscopy, which is produced with solvents based on mixtures of aliphatic hydrocarbons. It contains an aromatic-free substitute for xylene, thus, Neo-Mount® needs to be combined with Neo-Clear® exclusively. Xylene must be avoided in the mounting step, as it will cause the slides to become cloudy and streaked. In addition, by placing the dehydrated slides on filter paper for approx. 1 minute prior to mounting, any excess Neo-Clear® should be circumvented, as air bubbles might arise under the cover slip. The same pre-condition should also be met when mounting specimens using cover slip machines; in this area, Neo-Clear® can be most efficiently eliminated by incubating the slides for 1 minute in an empty slide rack.

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Tissue-clearing reagent, CUBIC-X1 for animals

Supplier: TCI

Specifications
Appearance: Colorless to Almost colorless clear liquid
Functionality test: to pass test (Remaining fluorescence strength)

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VWR® Q Path®, Slow Acting Decalcifiers

VWR® Q Path®, Slow Acting Decalcifiers

Supplier: VWR Chemicals

DC1 slow acting decalcifiers contain a mixture of formic acid and formalin.

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PMA enhancer for Gram negative bacteria solution 5X

Supplier: Biotium

PMA Enhancer for Gram Negative Bacteria was designed to improve PMA- and PMAxx™-mediated discrimination between live and dead gram-negative bacteria.

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CytoLiner™ Fixed Cell Membrane Stains

Supplier: Biotium

CytoLiner™ Fixed cell membrane dyes are novel lipophilic fluorescent dyes developed specifically for selective plasma membrane staining in fixed cells for microscopy. The dyes are engineered for robust and consistent staining of formaldehyde-fixed cells, and are suitable for downstream immunofluorescence staining protocols.

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Pierce™ Silver Stain Rescue Reagent

Supplier: Thermo Fisher Scientific

Pierce silver stain rescue reagent enables removal of undesirable background from unevenly and overly developed silver-stained polyacrylamide electrophoresis gels.

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Immersion oil

Immersion oil

Supplier: PanReac AppliChem

This fluorescence-free immersion oil for microscopy is highly UV-permeable and free of PCB's.

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Davidson solution

Davidson solution

Supplier: PanReac AppliChem

Davidson solution is a rapid fixative.

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Bouin liquor

Bouin liquor

Supplier: PanReac AppliChem

Fixative for preserving soft and delicate structures, used as a mordant in various trichrome procedures.

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FluoroQuest™ Mounting Medium with DAPI (50 ml)

FluoroQuest™ Mounting Medium with DAPI (50 ml)

Supplier: AAT Bioquest

FluoroQuest™ with DAPI is an aqueous mounting medium for preserving fluorescence of tissue and cell smears.

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ReadiUse™ Microscope Mounting Solution (50 ml)

ReadiUse™ Microscope Mounting Solution (50 ml)

Supplier: AAT Bioquest

The mounting medium is the solution in which the specimen is embedded, generally under a cover glass.

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FluoroQuest™ Antifade Mounting Medium

FluoroQuest™ Antifade Mounting Medium

Supplier: AAT Bioquest

FluoroQuest™ Antifade mounting medium is a specialised and stable formula that effectively preserves fluorescence by preventing rapid photobleaching of fluorescent proteins and dyes.

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FluoroQuest™ Fluorescence Signal Enhancing Solution (5 ml)

FluoroQuest™ Fluorescence Signal Enhancing Solution (5 ml)

Supplier: AAT Bioquest

When proteins are conjugated to fluorescent organic dyes, fluorescence emission of the dye molecules is usually decreased, sometimes up to 50 to 70%.

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FluoroQuest™ Anti-Fading Mounting Medium with DAPI (20 ml)

FluoroQuest™ Anti-Fading Mounting Medium with DAPI (20 ml)

Supplier: AAT Bioquest

FluoroQuest™ with DAPI is an aqueous mounting medium for preserving fluorescence of tissue and cell smears.

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FluoroQuest™ PLUS Antifade Mounting Medium

FluoroQuest™ PLUS Antifade Mounting Medium

Supplier: AAT Bioquest

FluoroQuest™ PLUS Antifade mounting medium, the latest breakthrough in non-setting media technology, provides unparalleled protection against fading throughout the visible spectrum, including far-red wavelengths.

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FluoroQuest™ Anti-Fading Kit I *Optimized for Slide Imaging* (1 Kit)

FluoroQuest™ Anti-Fading Kit I *Optimized for Slide Imaging* (1 Kit)

Supplier: AAT Bioquest

When exposed to excitation light, fluorescence intensity of dyes decreases due to their photooxidation or other photoreactions.

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FluoroQuest™ Anti-Fading Kit II *Optimized for Plate Imaging* (1 Kits)

FluoroQuest™ Anti-Fading Kit II *Optimized for Plate Imaging* (1 Kits)

Supplier: AAT Bioquest

When exposed to excitation light, fluorescence intensity of dyes decreases due to their photooxidation or other photoreactions.

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FluoroQuest™ TSA/PSA Antifade Mounting Medium *Optimised for Tyramide and Styramide Imaging*

FluoroQuest™ TSA/PSA Antifade Mounting Medium *Optimised for Tyramide and Styramide Imaging*

Supplier: AAT Bioquest

FluoroQuest™ TSA/PSA Antifade mounting medium is the only mounting medium that is optimised for tyramide (TSA) and styramide (PSA)-based fluorescence imaging applications.

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