You searched for: Proteinrenings
Proteinreningssystem är avsedda för isolering av ett eller flera proteiner från en komplex blandning. Oavsett om du arbetar med celler, vävnader eller hela organismer renar maskinerna med fördefinierade startpunkter och redigerbara metodmallar. De kompakt utformade proteinreningsapparater är enkelt att använda och har realtidskontroll och visualisering. De kan användas i kylrum för rening av affinitetsmärkta proteiner, antikroppar, omärkta eller nativa proteiner, samt provrengöring.
Multimodal chromatography media, Capto™ Core 700
Supplier: Cytiva
Capto™ Core 700 is designed for intermediate purification and polishing of viruses and other large biomolecules (molecular weight >700000).
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Affinity chromatography media, Dextrin Sepharose™ High Performance
Supplier: Cytiva
Dextrin Sepharose™ High Performance is an affinity media for purification of recombinant proteins tagged with Maltose Binding Protein (MBP).
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Gel filtration calibration kits
Supplier: Cytiva
Gel Filtration Calibration Kits are suitable for calibration of high-resolution gel filtration media and columns, such as Superdex, Superose and Sephacryl.
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Gelatin-Sepharose® 4B
Supplier: Cytiva
Gelatin Sepharose® 4B affinity media with immobilised gelatin is designed for the purification or depletion of fibronectin as gelatin binds to fibronectin with high specificity. Gelatin Sepharose® 4B is bovine gelatin coupled to Sepharose® 4B by the cyanogen bromide method.
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Affinity chromatography media, Streptavidin Sepharose™ High Performance
Supplier: Cytiva
Streptavidin Sepharose™ High Performance is an affinity chromatography medium designed for fast and reliable, high resolution purification of biotinylated biomolecules. Purified Streptavidin isolated from Streptomyces avidinii is immobilised on Sepharose™ High Performance.
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Accessories for chromatography systems
Supplier: Cytiva
Septum, silicone with PTFE backing
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Capto™ adhere ImpRes multimodal chromatography media, Cytiva
Supplier: Cytiva
Capto™ adhere ImpRes is a strong anion exchange multimodal BioProcess chromatography medium (resin), designed to meet the demands of modern large-scale manufacturers, for high-resolution polishing of monoclonal antibodies (MAbs) and other biomolecules. Capto™ adhere ImpRes is designed to allow effective MAb polishing in the second or third step of a purification scheme downstream of the protein A capture step. Polishing can be performed in either bind and elute (binding) or flowthrough (non binding) modes.
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Gel filtration media, Sephadex™ LH-20
Supplier: Cytiva
Sephadex™ LH-20 is a liquid chromatography media for molecular sizing of natural product such as steroid, terpenoid, lipid and low molecular weight peptides. Sephadex™ LH-20 is pre-pared by hydroxypropylation of Sephadex™ G-25, a bead-formed dextran medium.
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Gel filtration media, Sephadex™ G-10
Supplier: Cytiva
Sephadex™ G-10 is a well established gel filtration medium for desalting and buffer exchange of peptides and small biomolecules >700 molecular weight.
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Sepharose™ CL-4B Gel Filtration Base Matrix
Supplier: Cytiva
Sepharose™ CL-4B is a cross-linked agarose gel filtration base matrix and is frequently used for coupling affinity ligands to the matrix.
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Affinity chromatography media, Chelating Sepharose™ Fast Flow
Supplier: Cytiva
Chelating Sepharose Fast Flow is composed of cross-linked 6% agarose beads modified with iminodiacetic immobilized to the base matrix by stable ether linkages and sufficiently long spacer arms.
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Affinity chromatography media, Ni Sepharose™ 6 Fast Flow
Supplier: Cytiva
Ni Sepharose 6 Fast Flow is a BioProcess medium that combines the advantages of Ni₂₊ for purification of histidine tagged proteins, with the well industrially established properties of the Sepharose Fast Flow platform.
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Affinity chromatography media, MabSelect SuRe™
Supplier: Cytiva
MabSelect SuRe™ is composed of a rigid, high-flow agarose matrix and alkali-tolerant rProtein A ligand. The ligand has been engineered to provide greater stability than conventional protein A-based media in the alkaline conditions used in CIP protocols. This enhanced alkali stability improves process economy and product quality.
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Ion exchange chromatography media, DEAE Sephacel™
Supplier: Cytiva
DEAE Sephacel™ is a weak anion exchanger based on beaded cellulose. The ion exchange group is diethylaminoethyl, which remains charged and maintains consistently high capacity over the entire working range. This media is macroporous and has an exclusion limit of ~10000 kDa for globular proteins. Supplied pre-swollen in 20% ethanol.
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Affinity coupling media, Epoxy-Activated Sepharose™ 6B
Supplier: Cytiva
Epoxy-activated Sepharose™ 6B is a pre-activated medium for immobilisation of various ligands including sugars through coupling of hydroxy, amino or thiol groups on the ligand to Sepharose™ 6B via a 12-atom hydrophilic spacer arm. Medium is formed by reacting Sepharose™ 6B with 1,4-bis (2,3- epoxy-propoxy-) butane. It can be used to couple sugars and other carbohydrates via stable ether linkages to hydroxyl groups.
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CM Sephadex™ C-25 Ion Exchange Chromatography Media
Supplier: Cytiva
Sephadex ion exchangers are produced by introducing functional groups onto the cross-linked dextran matrix.
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Con A Sepharose™ 4B Affinity Chromatography Media, Cytiva
Supplier: Cytiva
Con A Sepharose is Concanavalin A coupled to Sepharose 4B by the cyanogen bromide method.
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Gel filtration media, Superdex™ 30 PG
Supplier: Cytiva
Superdex prep grade is a preparative gel filtration medium with a composite matrix of dextran and agarose
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Affinity chromatography media, MabSelect™
Supplier: Cytiva
MabSelect™ is a protein A affinity resin designed for capturing monoclonal antibodies (mAbs) from large sample volumes.
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Affinity chromatography media, Benzamidine Sepharose™ 4 Fast Flow (High Sub)
Supplier: Cytiva
Benzamidine Sepharose™ 4 Fast Flow (High Sub) is a tool for removal and/or purification of trypsin and trypsin-like serine proteases, as well as zymogens, including urokinase and prekallikrein.
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Hydrophobic interaction chromatography media, Butyl Sepharose™ 4 Fast Flow
Supplier: Cytiva
Butyl Sepharose 4 Fast Flow is part of the Sepharose Fast Flow HIC platform, which has been an industrial standard for HIC chromatography during recent decades.
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Sepharose™ CL-6B Gel Filtration Base Matrix
Supplier: Cytiva
Sepharose™ CL-6B is a cross-linked agarose gel filtration base matrix and is frequently used for coupling affinity ligands to the matrix.
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Plasmid purification media, PlasmidSelect Xtra
Supplier: Cytiva
PlasmidSelect Xtra is a thiophilic aromatic adsorption chromatography medium with a selectivity that allows generic purification of supercoiled covalently closed circular forms of plasmid DNA to be separated from open circular forms.
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Accessories for ÄKTA pure
Supplier: Cytiva
Tubing kit I. Ø. 0,25 mm, for ÄKTA pure 25
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Immobilised metal ion affinity chromatography (IMAC) media, Ni Sepharose™ excel
Supplier: Cytiva
Ni Sepharose™ excel is an IMAC medium pre-charged with nickel ions very strongly bound to a chelating ligand. Samples that usually cause stripping of metal ions can, therefore, be loaded to the medium. Ni Sepharose™ excel is designed primarily for capture and purification of histidine-tagged proteins secreted into cell culture supernatants from eukaryotic cells such as insect cells or CHO cells.
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Accessories and spare parts for ÄKTA start
Supplier: Cytiva
Frac30 bowl assembly
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Affinity chromatography media, Glutathione Sepharose™ 4 Fast Flow
Supplier: Cytiva
Glutathione Sepharose 4 Fast Flow is used for the purification of GST-tagged proteins from bacterial lysates and other S-tranferases or glutathione-dependent proteins.
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Affinity purification media, GammaBind™ Plus Sepharose™
Supplier: Cytiva
GammaBind™ Plus Sepharose™ is GammaBind G, type 3, covalently immobilised to Sepharose™ CL-6B by malimide linkage.
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Ion exchange chromatography media, SOURCE™ 15S
Supplier: Cytiva
SOURCE™ 15S is a synthetic high performance, preparative, chromatography medium, based on a 15 µm monosized, rigid polystyrene/divinyl benzene polymer matrix.
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2D Clean-Up kit
Supplier: Cytiva
The 2-D Clean-Up Kit is designed to prepare samples for 2-D electrophoresis that otherwise produce poor 2-D spot-maps due to high conductivity, high levels of interfering substances, or low protein concentration. The reagents quantitatively precipitate proteins while leaving interfering substances, such as detergents, salts, lipids, phenolics, and nucleic acids, in solution.