You searched for: Buffers

Supplier: ENZO LIFE SCIENCES

Neutralising buffer for low pH solutions and for use with cyclic nucleotide and isoprostane kits.

Supplier: Rockland Immunochemicals

20X SSPE buffer (3,0 M sodium chloride, 0,2 M sodium hydrogen phosphate, 0,02 M EDTA, pH 7,4) - DEPC treated.

Supplier: Biotium

Fish gelatin is commonly used in blocking solutions to minimise background noise in immunodetection procedures such as Western Blotting and immunostaining. Fish gelatin can be used to block non-specific binding sites on positively charged nylon or PVDF membranes or for blocking and dilution of antibodies during immunostaining procedures. Unlike BSA or milk, fish gelatin does not contain IgG or serum proteins that could cross-react with mammalian antibodies.

Supplier: Rockland Immunochemicals

This product was aseptically filtered through a Millipore 0,22 µm filter into clean, pre-sterilised containers. The product was tested on trypticase soy agar for 24 hours, 48 hours and 72 hours and was found to be negative for bacteria.

Supplier: ENZO LIFE SCIENCES

Hybridisation wash reagent provides a high quality hybridisation wash solution for in situ hybridisation analyses using DNA probes labeled with biotin, digoxigenin or fluorescein.

Supplier: ENZO LIFE SCIENCES

This Tween®-20 solution (10%) is specially designed to create the Blocking Buffer and Assay Buffer in ImmunoSet products. Each bottle is sufficient for use with 5×96 wells microtiter plates if following the instructions in our ImmunoSet protocol.

Supplier: Cayman Chemical

250 mM Tris-base, pH 7,4, 1,37 M sodium chloride, containing 27 mM KCl, 0,4 mM phenol red.

Supplier: Rockland Immunochemicals

Saline-sodium citrate (SSC) buffer is most commonly used as a buffer for the hybridisation process and washing in experiments where spcies of nucleic or ribonucleic acid are to be matched to complimentary sequences (such as Southern and Nouthern blotting, in situ hybridisation, or DNA microarrays). SSC is used to help control the stringency of hybridisation in these steps.

Supplier: TCI

This product contains 8% SDS, 40% glycerol, 0.02% bromophenol blue and 0.25 M Tris HCl, pH approx. 6.8. Does not contain 2-Mercaptoethanol.

Supplier: ENZO LIFE SCIENCES

50 mM Tris/Cl, pH 8.0, 137 mM NaCl, 2.7 mM KCl, 1 mM MgCl2, 1 mg/ml BSA.

Supplier: Biotium

A buffer set that blocks non-specific antibody binding as well as direct interaction of fluorescent dyes on antibodies with cells or tissue sections.

Supplier: ENZO LIFE SCIENCES

Solid. 100 ml PBS (1X) tablets containing 137 mM sodium chloride, 2.7 mM potassium chloride, and 10 mM phosphate buffer.

Supplier: Rockland Immunochemicals

SDS-PAGE, sodium dodecyl sulfate polyacrylamide gel electrophoresis, describes a technique widely used in biochemistry to separate proteins according to their electrophoretic mobility (a function of the length of a polypeptide chain and its charge) and no other physical feature. SDS is an anionic detergent applied to protein sample to linearise proteins and to impart a negative charge to linearised proteins.

Supplier: HyClone products (Cytiva)

Balanced salt solutions for cell culture applications.

Supplier: VWR Chemicals

The reagent is specially intended for use in testing Ph Eur and monographs. This solution was produced according 4.1.1 of Ph Eur specifications using Ph Eur reagent quality components and water meeting the Ph Eur monograph requirement for purified water.

Supplier: Thermo Fisher Scientific

CAS No.: 38641-82-6

Supplier: HyClone products (Cytiva)

Balanced salt solution used for multiple cell culture applications (powder form).

Supplier: Cayman Chemical

Sodium phosphate stock solution (0,5 mol/L), pH value: 7,0

Supplier: AGILENT

Progress from mammalian cells to qPCR or QRT-PCR experiments without nucleic acid purification.

Supplier: Rockland Immunochemicals

This product is ready-to-use as a working 1X solution and requires no further dilution. This buffer consists of 10 mM Tris HCl, 100 mM NaCl, and 1,0 mM EDTA at a pH of 7,8. Meticulously prepared using ultra pure reagents dissolved in DEPC treated pharmaceutical grade deionised water.

Supplier: Avantor

DILUT-IT is a ready-to-use dissolution media concentrate, which is pre-measured and only need to be diluted with purified water.

Supplier: G-Biosciences

BLOT-QuickBlocker™ is a modified milk protein that is highly soluble and does not inhibit peroxidase detection. The modified milk protein has high blocking efficiency with a clear background.

Supplier: Avantor

J.T. Baker® Cell Lysis Solution. Optimized multi-component, ready-to-use solution for extraction and recovery of AAV particles from HEK293 or SF9 cells. The product offers quantitative recovery through efficient cell lysis and protection of vector particles against shear stress. The solution is formulated with non-ionic detergents which are octyl phenol ethoxylate free.

Supplier: TCI

Form: White granules

Supplier: FUJIFILM WAKO CHEMICALS

These depyrogenated dilution tubes are made of high-quality borosilicate glass depyrogenated at 250 °C.

Supplier: Avantor

J.T.Baker® Cell Lysis Solution Biotech Reagent is a cGMP pre-formulated and ready to use detergent optimized for the extraction and recovery of AAV particles from producer cell lines (HEK293 parental and suspension lines, SF9). J.T.Baker® Cell Lysis Solution is a proprietary blend of nonionic surfactants, free of octyl phenol ethoxylate, which demonstrates comparable performance industry standard detergents while addressing environmental concerns associated with REACH and downstream clearance concerns. Designed for Gene Therapy workflows, this formulation is easy to use and handle as it is ready-to-use, has low viscosity, does not require cold temperature shipping or storage, and is available in sizes to support processes from development to large scale commercial batches. Detail description provided in technical literature.

Supplier: 3M Food Safety

The 3M™ Mini Flip-Top Vial with 9 ml Maximum Recovery Diluent is a leak-resistant plastic cylinder with a flip-open lid that seals securely for safe shipping and handling.

Supplier: FUJIFILM WAKO CHEMICALS

These Gel-clot Reaction Tubes are made of high-quality borosilicate glass, depyrogenated at 250 °C. This results in an endotoxin-free tube that is designed to perform successful LAL gel clot assays with heat blocks and water baths.

Supplier: OMEGA BIO-TEK

The E.Z.N.A.® RNALock Stabilizer Reagent is an innovative reagent which rapidly lyses cells and tissues to inactivate RNases at room temperature. Simply submerge the samples, such as cell cultures, animal tissues, or white blood cells into the RNALock Stabilizer Reagent. The intact cellular RNA will be preserved even at elevated temperature. Samples can be stored in this reagent in excess of 12 months at −20 °C.

Supplier: PanReac AppliChem

Cloning Buffer, TE buffer solution (TRIS-EDTA buffer) pH 8,0