Resisting pH changes, buffers protect samples when acids or alkali is added. Powder and liquid forms efficiently track proteins during electrophoresis isolation and separation. The salty buffers can also be used to calibrate and standardize pH meters for accurate readings. Blocking substances stop antibody attachment to membrane free sites that would produce an interfering background. In various concentrations to fit any need, the buffers may come in liquids or dehydrated formats.
TM Buffer, 1X Tris-Magnesium sulphate, pH 7,5
Supplier: Rockland Immunochemicals
This product is ready-to-use as a working 1X solution and requires no further dilution. This buffer consists of 50 mM Tris HCl, 10 mM magnesium sulphate at pH 7,5 and was prepared using ultra pure reagents dissolved in pharmaceutical grade deionised water.
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Protein blocking buffers, Superior™
Supplier: G-Biosciences
Blocking buffer is an enhanced blocking agent available in multiple formats. Superior™ Blocking Buffer contains a proprietary antigenically non-determinant protein for blocking Western blot membranes, blocking and storage of ELISA plates, and blocking prior to immunohistochemistry staining.
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MMP-1 colorimetric assay buffer
Supplier: ENZO LIFE SCIENCES
MMP-1 colorimetric assay buffer
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Caspase cell lysis buffer
Supplier: ENZO LIFE SCIENCES
Caspase cell lysis buffer, Enzo Life Sciences
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PBS and TBS buffers
Supplier: Rockland Immunochemicals
Phosphate buffered and Tris buffered saline are suitable for multiple applications including biological diluent buffer for antibodies or other biologics. Also may be used as a wash buffer for immunological assays including western blot, immunohistochemistry, immunofluorescence microscopy, and ELISA. Other applications may require detergents or other additional components.
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Buffer, TRIS solution pH 7 (1 mol/L)
Supplier: Cayman Chemical
Cloning Buffer, TRIS buffer solution pH 7 (1 mol/L)
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Buffer, PBS tablets
Supplier: PanReac AppliChem
PBS Tablets for preparation of 200 ml phosphate buffered saline (PBS). Composition of 140 mM sodium chloride, 2,7 mM potassium chloride and 10 mM phosphate.
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ES Buffer
Supplier: FUJIFILM WAKO CHEMICALS
The ES Buffer is an endotoxin-specific buffer that is ideal for untreated LAL.
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Mini flip-top vial with 9 ml buffered peptone water broth, BPPFV9BPW
Supplier: 3M Food Safety
The 3M™ Mini Flip-Top Vial with 9 ml Buffered Peptone Water Broth is a leak-resistant plastic cylinder with a flip-open lid that seals securely for safe shipping and handling.
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Buffer, TBS solution 10X concentrate (TRIS buffered saline) pH 7,4
Supplier: Cayman Chemical
Formulation: Concentrated TBS (250 mM Tris-base; pH 7,4; 1,37 M sodium chloride and 27 mM KCl).
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Buffer, TBS solution 10X concentrate (TRIS buffered saline)
Supplier: G-Biosciences
The TBS solution is primarily used as a wash and blocking buffer diluent for Western blot, ELISA and immunoassays.
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In-situ hybridisation buffer for HPV probes
Supplier: ENZO LIFE SCIENCES
In situ hybridisation buffer for HPV probes (ready-to use) provides a high quality hybridisation medium for In situ hybridisation analyses using either DNA or oligonucleotide probes labeled with biotin-, or fluorescein-modified nucleotides.
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Buffer, PBS (10X, Dulbecco's) powder pack
Supplier: PanReac AppliChem
Powder mixture to prepare PBS buffer (10X) according to Dulbecco.
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HEPES Buffer Solution 1 M, HyClone™
Supplier: HyClone products (Cytiva)
Maintain physiological pH in cell culture, despite changes in carbon dioxide concentration (produced by cellular respiration) with Cytiva HyClone HEPES solution.
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TISAB II Solution, Supelco®
Supplier: Merck
TISAB, in general, is mixed with samples, as it helps in maintaining the ionic strength and pH constant. It is mainly used to decomplex metal-F complexes.
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Cell culture salts, salt solutions and buffers
Supplier: BIOWEST
These irrigating buffers and salt solutions are sterile, physiological balanced solutions intended for use in the maintenance of mammalian cells, where a chemically defined, balanced salt solution provides an environment that will maintain the structural and physiological integrity of cells in vitro. Applications include the intermediate steps for cultivation of cells, like washing, centrifugation, suspending and counting, as well as for many analytical methods or biochemical treatments. Principally these solutions should be used to keep the cells in suspension only for minutes or a few hours maximum. These buffers and solutions are not cell culture media.
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Buffer kit for calibration of fluorescent Ca² indicators
Supplier: Biotium
Kit provides a range of calibration buffers with accurate calcium concentrations and is useful for the calibration of fluorescent Ca² indicators (1,2).
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VWR®, Buffers, ammonia- ammonium chloride and ammonium chloride-ammonium hydroxide USP Test solutions (TS)
Supplier: VWR Chemicals
Ready to use test solutions (TS).
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Caspase assay buffer
Supplier: ENZO LIFE SCIENCES
50 mM HEPES, pH 7,4, 100 mM NaCl, 0,1% CHAPS, 10 mM DTT, 1 mM EDTA, 10% glycerol.
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WB blocking buffer kit, TrueBlack®
Supplier: Biotium
The TrueBlack® WB blocking buffer kit is a ready-to-use buffer system that provides optimal specificity, sensitivity, and background signal suppression for fluorescence-based western blotting (WB).
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TPE buffer, 10X tris-phosphate-EDTA, pH 8
Supplier: Rockland Immunochemicals
Running Buffer, Tris-phosphate-EDTA 10X (TPE) (0,9 M tris-phosphate 0,01 M EDTA pH 8,0)
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Extraction reagents
Supplier: ENZO LIFE SCIENCES
Concentrated lysis buffer for preparation of cell and tissue extracts.
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PBS (endotoxin-free), Enzo Life Sciences
Supplier: ENZO LIFE SCIENCES
Sterile and endotoxin-free PBS for use with TLRgrade™ or endotoxin-free grade reagents.
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Blocking buffers, Protein-Free™
Supplier: G-Biosciences
Protein-Free™ Blocking buffer is a formulation of non-protein agents that eliminate non-specific binding sites in ELISA, blotting, immunohistochemistry and other applications. The absence of protein from the Protein-Free™ Blocking buffer eliminates problems associated with traditional protein based blockers, such as cross-reactivity and interference from glycosylated proteins.
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Buffer HF-Improved
Supplier: Thermo Fisher Scientific
Buffer used for pretreatment of planar silicon devices when plating with nickel plating solution products 44069 and 44070.
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Optimizer Buffer™ for optimal cross-linking and modification
Supplier: G-Biosciences
The conjugation and modification reactions used to cross-link proteins or couple labels to proteins, such as biotin, enzymes, and fluorescent dyes, require certain conditions, including pH and chemical composition for optimal conjugation. Many common buffers routinely used in laboratories have an inhibitory effect on conjugation reactions; for example, Tris buffers inhibit coupling to amines.
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Cell lysis buffer 4, (100 ml), Enzo Life Sciences
Supplier: ENZO LIFE SCIENCES
Cell lysis buffer 4, Enzo Life Sciences
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Non-animal protein blocking Agent, NAP-BLOCKER™
Supplier: G-Biosciences
NAP-BLOCKER™ is an animal-free protein blocking agent recommended for improved assay sensitivity, minimal non specific binding and a high signal-to-background ratio. It is suitable for Western blots, dot blots and assay development.
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GET buffer
Supplier: Rockland Immunochemicals
GET buffer (50 mM Glucose, 10 mM EDTA, 25 mM Tris HCl, pH 8,0) is used in effective plasmid miniprep.